High night-time temperature during flowering and pod filling affects flower opening,yield and seed fatty acid composition in canola     

Meghnath Pokharel  Anuj Chiluwal  Michael Stamm  Doohong Min  Davina Rhodes  S.V. Krishna Jagadish 《Journal of Agronomy and Crop Science》2020,206(5):579-596

Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future.  相似文献   

Prevalence of Salmonella antibodies among goats slaughtered for chevon in Bareilly (Northern India)     

Chandra M  Singh BR  Shankar H  Agarwal M  Agarwal RK  Sharma G  Babu N 《Preventive veterinary medicine》2007,80(1):1-8

We screened serum samples of 1024 goats slaughtered for chevon in Bareilly in Northern India for Salmonella antibodies with indirect ELISA, MAT-H (microagglutination test using flagellar antigens e, n, x and 1, 5) and MAT-O (microagglutination test using somatic antigens 4, 12 and 3, 10, 15). Salmonella antibodies were detected in 48, 8 and 40%, goats using Salmonella-cytotoxi-I ELISA, MAT 'H' and MAT 'O', respectively. After adjusting for test accuracy, the seroprevalence were highest for Salmonella-cytotoxi-I ELISA (46%) followed by agglutinins against 'O' 3, 10, 15 (15%) and negligible for other agglutinins. With all 5 tests, prevalence of Salmonella antibodies was significantly higher in females than in males. No significant difference was evident in prevalence of Salmonella antibodies to different antigens in different age groups of male goats except for e, n, x agglutinins that were significantly more prevalent in young adult (<6-18 months) males than in adult (>18 months of age) or young (< or =6 months of age) goats. On the other hand, in females, prevalence of Salmonella-cytotoxin-I antibodies and e, n, x agglutinins differed significantly among three age groups, being the most prevalent in adult goats. As expected, the results of different tests had little or no correlation because the different tests targeted antibodies to different antigens.  相似文献   

Classification of Dutch and German avian reoviruses by sequencing the sigma C protein   总被引：1，自引：0，他引：1  

Kant A  Balk F  Born L  van Roozelaar D  Heijmans J  Gielkens A  ter Huurne A 《Veterinary research》2003,34(2):203-212

We have amplified, cloned and sequenced (part of) the open reading frame of the S1 segment encoding the sigma C protein of avian reoviruses isolated from chickens with different disease conditions in Germany and The Netherlands during 1980 up to 2000. These avian reoviruses were analysed phylogenetically and compared with sequences of avian reoviruses in the Genbank database. The avian reoviruses could be grouped in 5 different genotyping clusters and this classification was identical when the sequences were compared of the 5' end, the 3' end or the whole open reading frame of the sigma C protein. Therefore sequencing of either part of the gene encoding the sigma C protein seems to be reliable for classification. We were unable to identify a correlation between sigma C sequences of the avian reoviruses and the disease condition they were isolated from. The sequences found in The Netherlands and in Germany are, like those in Taiwan, more dispersed than the known avian reovirus sigma C sequences in the USA and Australia. We did not establish temporal or geographic differences in the avian reoviruses studied.  相似文献   

Resistance to early blight of tomato with respect to various parameters of disease epidemics   总被引：3，自引：1，他引：2  

Koshlendra Kumar?PandeyEmail author  Padma Kant?Pandey  Gautam?Kalloo  Malay Kumar?Banerjee 《Journal of General Plant Pathology》2003,69(6):364-371

Resistance to early blight in the tomato was assessed by examining various parameters of the progress of the disease. Artificial inoculation and the scoring technique were standardized. Test plants were inoculated with 125cfu/ml of a 12-day-old culture of a pathogenic isolate of Alternaria solani. Screening under artificial conditions was more informative than that under natural epidemic conditions. Tomato cultivars CLN-2071-C, CLN-2070-A, BSS-174, and DTH-7 with resistance expressed as slow blighting against four pathogenic isolates of A. solani, were selected for cultivation in disease-prone areas. Disease intensity increased with the age of plants under the same inoculum load. The area under the disease progress curve (AUDPC) was positively correlated with the percentage disease index and negatively with resistance. Calculation of the apparent infection rate (r) was more informative for natural epidemics than for artificial conditions. The sequential apparent infection rate between observation periods was better correlated with disease progress than was the total apparent infection rate between the first and last observations. A double sigmoidal disease progress curve during the same cropping season was characteristic of some varieties when fungal infection took place during the vegetative phase of crop growth.  相似文献   

Studies on the pathogenesis of chicken infectious anaemia virus infection in six-week-old SPF chickens     

Drén CN  Kant A  Van Roozelaar DJ  Hartog L  Noteborn MH  Koch G 《Acta veterinaria Hungarica》2000,48(4):455-467

The pathogenesis of chicken infectious anaemia virus (CAV) infection was studied in 6-week-old and one-day-old SPF chickens inoculated intramuscularly with graded doses of Cux-1 strain (10(6)-10(2) TCID50/chicken). Viraemia, virus shedding, development of virus neutralizing (VN) antibodies and CAV distribution in the thymus were studied by virus isolation, polymerase chain reaction (PCR), immunocytochemistry (IP) and in situ hybridization until postinfection day (PID) 28. In 6-week-old chickens infected with high doses of CAV, viraemia and VN antibodies could be detected 4 PID and onward without virus shedding or contact transmission to sentinel birds. However, virus shedding and contact transmission were demonstrated in one-day-old infected chickens. In the 6-week-old groups infected with lower doses, VN antibodies developed by PID 14, transient viraemia and virus shedding were detected. The thymus cortex of all 1-day-old inoculated chickens stained with VP3-specific mAb. Cells with positive in situ hybridization signal were fewer and scattered throughout the thymus tissue of the one-day-old inoculated chickens as compared to IP-positive cells. These results suggest that early immune response induced by high doses of CAV in 6-week-old chickens curtails viral replication and prevents virus shedding.  相似文献   

Relationship between light,fruit and leaf mineral content with albinism incidence in strawberry (Fragaria x ananassa Duch.)     

R.R. Sharma  V.B. PatelHare Krishna 《Scientia Horticulturae》2006

A study was conducted in strawberry to establish a relationship between shading, mineral nutrient of leaves and fruits with albinism incidence. Plants grown under shade produced albino fruits in higher proportion than those grown in open fields. Similarly, plants under shade produced smaller sized fruits and have lower fruit yield. Among cultivars, Etna had highest incidence of albinism (49.6%) and Sweet Charlie the lowest (14.4%). Dry matter content (%), concentration of five major nutrients, viz. N, P, K, Ca, Mg, S and N:Ca and K:Ca nutrient ratios did not differ significantly in the leaves of plants producing normal or albino fruits. However, in contrast, the concentration of K was notably higher (1.97 mg g⁻¹ fresh tissue weight) and that of Ca was lower (0.098 mg g⁻¹ fresh tissue weight) in albino fruits than normal ones. Consequently, the ratios of N:Ca (11.34) and K:Ca (20.08) were higher in albino fruits than normal ones. Cultivars also differed widely in respect to dry matter (%), mineral content and nutrient ratios. Thus, it appears that lower light intensity favours the development of albinism in strawberry, and it seems that calcium is not the basic cause of albinism, but increased vigour associated with overuse of N and K might be positively associated with it.  相似文献   

Zaluzanin D: a fungistatic sesquiterpene from Vernonia arborea     

Krishna Kumari GN  Masilamani S  Ganesh MR  Aravind S  Sridhar SR 《Fitoterapia》2003,74(5):479-482

Zaluzanin D, a sesquiterpene lactone has been isolated from the aerial parts of Vernonia arborea as a major constituent (0.04%). Antifungal activity of zaluzanin D has been studied against six plant-pathogenic fungi.  相似文献   

Seed germination and seedling development in the mango (Mangifera indica L.)     

Corbineau F  Kanté M  Côme D 《Tree physiology》1986,1(2):151-160

Mango (Mangifera indica L., cv Ruby) seeds taken from ripe fruit showed no dormancy. They germinated at temperatures between 5 and 40 degrees C, but germination was most rapid near the upper end of this range (25-40 degrees C). The fresh seeds had a high moisture content (85%, dry weight basis) and quickly died on dehydration. The optimal temperature for growth of the seedlings was close to 30 degrees C. High temperatures (40 degrees C) and temperatures below 15 degrees C were lethal. Growth of the stem occurred in successive flushes separated by rest periods. When the leaves of the preceding flush finished growing, the axis lengthened beneath the apical bud.  相似文献   

Utilization of Weather Data in Predicting Bread Loaf Volume by Neural Network Method     

David Karki  Karl D. Glover  Krishna Bondalapati  Padmanaban G. Krishnan 《Cereal Chemistry》2016,93(5):471-477

The unique ability of wheat (Triticum aestivum L.) flour to produce cohesive dough has helped to make it the most widely used cereal crop for bread and other baked food products. Measurement of end‐use qualities, such as loaf volume, is ideally carried out through assessing loaves of bread; however, this is resource intensive. Predictive testing methods are more often utilized to identify wheat genotypes with potentially acceptable loaf volume, although more accurate predictive methods would be beneficial. Our objectives were to study the influence of weather observations on bread loaf volume and flour and dough quality data, to use a neural network (NN) model to predict loaf volume with select input data, and to compare the best multiple regression models identified with their NN counterparts. Weather data collected at 20 days after heading (DAH) showed the highest correlations with bread loaf volume when compared with prior intervals. A NN model containing maximum, minimum, and nighttime temperatures produced the highest coefficient of determination for predicting loaf volume. Our results showed that the NN model explained up to 20% more loaf volume variation than a similar regression model. Weather parameters representing conditions at 20 DAH played a significant role in loaf volume prediction.  相似文献   

Ecological and anthropogenic niches of sal (Shorea robusta Gaertn. f.) forest and prospects for multiple-product forest management - a review     

Gautam  Krishna H.; Devoe  Nora N. 《Forestry》2006,79(1):81-101

Sal (Shorea robusta Gaertn. f.) forests cover over 11 millionha in India, Nepal and Bangladesh, and these forests are conventionallymanaged for timber. Recently, interest in producing multipleproducts from sal forests has increased; accordingly, a silviculturalregime for managing sal forest for multiple products is a centralconcern. Forest managers need a comprehensive scientific understandingof natural stand development processes and anthropogenic factorsaffecting sal forest when designing silvicultural regimes formultiple-product management. We review ecology and productivityplus anthropogenic niches of sal forests. Information on edaphicfactors, phenology and stand development processes (regeneration,growth characteristics, soil nutrient requirement, growth allocation,nutrient cycling, stand structure and successional stages) isimportant for designing scientific forest management of salforest; likewise, knowledge of anthropogenic factors associatedwith use of sal forest is also required for effective implementationof the recently paradigmed management efforts. Sal forest silviculturehas been evolving since the beginning of the twentieth centurymainly concentrating on timber production, though the sal forestshave always been used also for grazing and collection of fodder,fuelwood, litter and many other products. Instead of integratingthese products in sal forest management, governments have attemptedto control these additional uses through enforcing forest legislation.These attempts resulted in the persistent conflicts betweenthe interests of local people and the government, and the deterioratingcondition of sal forests. Community-based forestry in this regionemerged in response to the severe degradation of forest resources,and local people initiated protection practices and demonstratedthe success of sal forest from coppice. The coppice systemsallow managing forests with intermittent products (non-timberforest products, including fodder and litter) while producingtimber in the long term. Accordingly, a policy has been developedto manage coppice sal forest for multiple products. Managingthe sal forest for multiple products is, however, a relativelyrecent development and scientific investigations on variousaspects of multiple-product forest management need to be initiated.Ecological processes indicate good prospects of managing salforest for multiple products. The review indicates that theecological processes and anthropogenic factors form sound basisfor developing multiple-product management.  相似文献