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The phenotypic performance of a genotype is not necessarily the same under diverse agro-ecological conditions. Two commercial wheat cultivars and 33 wheat landrace varieties were selected for screening drought tolerance and the investigation of genotype × environment interactions (GEI). The study site experiences drought stress at reproductive stages. Fully irrigated along with two drought stress trials at early heading (EHS) and 50% heading stages (HDS) were performed in 2 years. The highest antioxidant and proline contents were accumulated under EHS. Reduction in cell membrane stability in EHS was 90.9% in 2011–2012. The lowest grain yield belonged to EHS (3.2 t ha?1) while the highest (6.8 t ha?1) was observed in the irrigated trial. GEI analysis indicated that the Pinthus’s coefficient was not an efficient index for screening GEI. Positive correlations were found between GEI parameters such as regression deviation, Wricke’s ecovalence and Shukla’s variance. Regression coefficients over trials ranged from 0.65 to 1.42, indicating genotypes had different responses to environmental changes. Regression models, GEI variances and grain yield showed that some varieties (KC4557, KC4862, KC3891, KC4495, and KC4633) had higher stability under drought stress and fully irrigated conditions and can be involved in breeding programs for drought stress tolerance.  相似文献   
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