Structure and Expression In planta of Botrytis cinerea Ubiquitin Genes     

Theo W. Prins  Lia Wagemakers  Jan A.L. van Kan 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(7):693-698

To identify genes of the necrotrophic pathogenic fungus Botrytis cinerea that are expressed during infection of tomato leaves, a differential screening of a genomic library with radioactively labelled cDNA was performed. This resulted in the identification of a B. cinerea gene, denominated Bcubi4, which encodes a precursor protein consisting of four identical head-to-tail repeats of a 76aa ubiquitin unit. Subsequently a gene denominated Bcubi1CEP79, encoding a single ubiquitin unit joined to a Carboxyl Extension Protein of 79 amino acids, was isolated. The expression of the two ubiquitin genes was studied during pathogenesis of B. cinerea on tomato. Bcubi1CEP79, but not Bcubi4, mRNA was transiently induced at 16h after inoculation. The increased expression of the Bcubi1CEP79 gene at this stage of pathogenesis might be required for enhanced ribosomal biogenesis.  相似文献   

‘Candidatus Phytoplasma australiense’ is the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases     

Lia W. Liefting  Anna C. Padovan  Karen S. Gibb  Ross E. Beever  Mark T. Andersen  Richard D. Newcomb  David L. Beck  Richard L.S. Forster 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):619-623

Sequence comparisons and phylogenetic analysis of the 16S rRNA genes and the 16S/23S spacer regions of the phytoplasmas associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases revealed minimal nucleotide differences between them resulting in the formation of a monophyletic group. Therefore, along with Australian grapevine yellows, the phytoplasmas associated with Phormium yellow leaf and papaya dieback should also be considered as Candidatus Phytoplasma australiense.  相似文献   

An easy,accurate and efficient procedure to create forest fire risk maps using the SEXTANTE plugin Modeler     

Lia Duarte  Ana Cláudia Teododo 《林业研究》2016,27(6):1361-1372

To prevent, detect, and protect against forest fires, forest personnel need to define rules for determining forest fire risk. In Portugal, all municipalities must annually produce forest fire risk(FFR) maps. To produce more reliable FFR maps more easily, we developed an open source model using the Modeler plugin of SEXTANTE in the program QGIS version 2.0 Dufour. The model provides all the maps involved in the FFR model(susceptibility map, hazard map, vulnerability map, economic value map,and potential loss map) and was produced according to Portuguese Forest Authority's(AFN, Autoridade Florestal Nacional) rules for determining the FFR. This model was tested for the Portuguese municipality Santa Maria da Feira, where 40 % of the total municipality area falls in the category ‘‘very high' or ‘‘high' fire risk. The ‘‘very high'fire risk area is mainly classified as broad-leaved forest and has the steepest slopes(15 %). The distance of burned areas to roads was also analyzed; the proportion of burned areas increased with increasing distance to the main roads.In addition, 92.6 % of the ‘‘high' and ‘‘very high' risk zones were located in areas with lower elevation. These results confirmed that forest fire is strongly influenced not only by environmental factors but also by anthropogenic factors. The procedure implemented here was compared with our open source application already available in QGIS and also to the same procedure implemented in GIS proprietary software. Although the results were obviously the same, the model developed here presents several advantages over the other two approaches. Besides being faster,it is easy to change the model parameters according to user needs(i.e., to the rules of different countries), and can be modified and adapted to other variables and other areas to create risk maps for different natural phenomena(e.g.,floods, earthquakes, landslides). The model is easy to use and to create risk and hazard maps rapidly in a free, open source environment that does not require any programming knowledge.  相似文献   

Effects of melatonin implants on reproduction and growth of turbot broodstock     

J.M.R. Alvariño  P.G. Rebollar  M. Olmedo  B. Alvarez-Blázquez  E. Ubilla  J.B. Peleteiro 《Aquaculture International》2001,9(6):477-487

The effect of 18 mg. melatonin (MLT) implants on thereproductive performance of turbot (Scophthalmus maximusL.) broodstock was determined in 20 females and 12 males. Implantedanimals showed supra-physiological plasma MLT concentrations during threemonthsafter implantation, being at that time about one hundred times higher thandiurnal MLT concentrations. Direct effects of MLT implants were observed ongonadal maturation of turbot females three months after implantation (P <0.01), although the faster ovarian response to increasing photoperiod was notconsistent and no differences were detected at the final reproductiveperformance. MLT implants had a positive effect on turbot male reproductivefeatures, since gonadal recrudescence started 45 days earlier (P < 0.05)showing a trend towards higher spermatozoa concentration. Further research isadvisable to ascertain the MLT dose required for and time of implantation inturbot broodstock.  相似文献   

The effect of column purification on cDNA indirect labelling for microarrays     

M Lia Molas  John Z Kiss 《Plant methods》2007,3(1):1-8

Background

The success of the microarray reproducibility is dependent upon the performance of standardized procedures. Since the introduction of microarray technology for the analysis of global gene expression, reproducibility of results among different laboratories has been a major problem. Two of the main contributors to this variability are the use of different microarray platforms and different laboratory practices. In this paper, we address the latter question in terms of how variation in one of the steps of a labelling procedure affects the cDNA product prior to microarray hybridization.

Results

We used a standard procedure to label cDNA for microarray hybridization and employed different types of column chromatography for cDNA purification. After purifying labelled cDNA, we used the Agilent 2100 Bioanalyzer and agarose gel electrophoresis to assess the quality of the labelled cDNA before its hybridization onto a microarray platform. There were major differences in the cDNA profile (i.e. cDNA fragment lengths and abundance) as a result of using four different columns for purification. In addition, different columns have different efficiencies to remove rRNA contamination. This study indicates that the appropriate column to use in this type of protocol has to be experimentally determined. Finally, we present new evidence establishing the importance of testing the method of purification used during an indirect labelling procedure. Our results confirm the importance of assessing the quality of the sample in the labelling procedure prior to hybridization onto a microarray platform.

Conclusion

Standardization of column purification systems to be used in labelling procedures will improve the reproducibility of microarray results among different laboratories. In addition, implementation of a quality control check point of the labelled samples prior to microarray hybridization will prevent hybridizing a poor quality sample to expensive micorarrays.  相似文献   

Interference elimination of PCB and its ramification in determining trace PBBs/PBDEs by GC/MS     

HUA Li  ZHANG Hong-quan  ZHANG Lei  LIU Tao  HUANG Yuan-qiao 《保鲜与加工》2011,(7):137-146

The normal method to determine the trace brominated flame retardants such as polybrominated biphenyls (PBBs) and polybrominated diphenylethers (PBDEs) contained in the electronic and electrical products is gas chromatography/mass spectrometry (GC/MS). However, the method is based on the determinations of the persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs), and polychlorinated naphthalene (PCNs) etc. The interferences of those compounds are inevitable. Therefore, in order to overcome this problem, two methods e.g. 1) The combination use of gas chromatography negative ion chemical ionization mass spectrometry (GC-NICI-MS) and gas chromatography-electron impact-mass spectrometry (GC-EI-MS) is employed, 2) a matrix solid-phase dispersion (MSPD) method with YMC ODS-C18 as carrier is developed. The result show that, by the former method, the brominated isomers or ramifications are distinguished remarkably from other halogen compounds because that anion fragment retention peaks of ［Br］-, ［HBr2］- and molecule chain fragment retention peaks of ［M+2］+,［M+4］+,［M+6］+,［M+8］+ are observed simultaneously, and thus the selectivity to determine bromine-containing retardant flames is greatly improved. Using the latter method, the gas chromatographic peaks of multiple polychlorinated biphenyls and polybrominated diphenylethers can be efficiently separated. Thus provides a project to solve interferences of POPs in brominated flame retardants’ determinations. The standard addition experimental results of 10 kinds of BDEs/PCBs belonging to 8 sorts of electronic and electrical equipment show this method has a high precision and reliability due to 60%～98% recovery and <9.5% relative error, which meet the needs regulated by the IEC Commission. It provides a technical support for electronic and eletrical industries in China to further comply with RoHS directive.  相似文献   

杉木不稔种子流行的数量化预测模型   总被引：2，自引：4，他引：2  

林思祖  林如兰 《福建林学院学报》1990,10(1):72-78

本文于尤溪县经营林场杉木种子园随机调查了303个球果的不稔种子流行率,选用12个探索性的因素作为项目(自变量),根据数量化理论Ⅰ原理,分别建立两个不稔种子(涩籽、瘪籽)流行的预测模型。复相关系数F检验表明观察F值大于1％F值,这两个模型皆可用于预报涩、瘪籽流行率。偏相关t检验表明遗传、立地、病害等对杉木不稔种子流行皆有明显的影响。  相似文献   

Vision and development in Trichoderma atroviride          下载免费PDF全文

Casas S　 Cortés C　 Ríos M　 Rosales T　 Bibbins M　 Olmedo V　 Herrera-Estrella A 《浙江大学学报(农业与生命科学版)》2004,30(4):390-390

Phototropism, the induction of carotenogenesis and reproductive structures, and resetting of the circadian rhythm are controlled by blue light. Trichoderma is used as a photomorphogenetic model due to its ability to conidiate upon exposure to light. In total darkness, T. atroviride grows indefinitely as a mycelium provided that nutrients are not limiting. However, nutrient deprivation and light trigger the conidiation process. A pulse of blue light given to a radially growing colony induc…  相似文献   

改革助推兵团走上生态与民生林业可持续发展之路——新疆生产建设兵团63团、71团林业改革调研报告     

张蕾  苗启华  齐联  陈谦  刘家顺  赖煜  刘霞 《绿色中国(A版)》2013,(10):3

通过对新疆生产建设兵团63团、71团林业改革的调研,分析了兵团林业改革面临的新形势和历史背景,总结了其主要做法和成效,并得出了3个方面的启示,即:生态林业民生林业共同发展是林业改革成功与否的标准,改革是重新调整生产要素的重要举措,改革应该是全方位系统性的。  相似文献   

Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp     

Boot R  Van de Berg L  Reubsaet FA  Vlemminx MJ 《Veterinary microbiology》2008,128(3-4):395-399

Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.  相似文献