Differentiation of influenza virus antigens using enzyme immunoassay]     

H Herrmann 《Archiv fuer experimentelle veterinaermedizin》1978,32(4):489-494

Reported is a microprocess of enzyme immune assay in which slipformed air pockets of polyvinylchloride (PVC), as used in the pharmaceutical industry, are used as carriers of antigens or antibody. Two methods, the anti-globulin and the double-antibody methods, are based on antibody which had been coupled with alkaline phosphatase. Tests in which various sub-types of influenza virus were used have shown the double-antibody method to be a sensitive technique which can be successfully used in the differentiation of envelope antigens.  相似文献   

Applied anatomy of the distal "vinculum tendinis" in the fetlock tendon sheath of the hindlimb in cattle]     

H Waibl  J Herrmann  J Rehage  P Lorenzi  G Constantinescu 《DTW. Deutsche tier?rztliche Wochenschrift》2001,108(6):261-263

A relatively thick (diameter approximately 2 mm), ropelike (length ca. 20 mm) and elastic "Vinculum tendinis" connects--within the fetlock tendon sheath--the dorsal side of the deep digital flexor tendon with the dorsal part of the Manica flexoria (the communicating band of the Musculus interosseous medius to the superficial digital flexor tendon). The extensive fetlock tendon sheath can be involved in diseases such as aseptic and septic inflammations. Spreading of these inflammations makes in some of these cases the partial resection of the tendon of the deep digital flexor muscle and the cutting of these Vincula necessary. The results of this contribution, collected from 60 hindlimbs of adult bovines show variations in number, length, diameter and extent and the inner structure with blood vessels and nerves.  相似文献   

Pathology, pathogenesis and etiology of osteoarthropathies in the swine     

H J Herrmann 《Archiv fuer experimentelle veterinaermedizin》1972,26(4):617-644

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Differentiation of matrix proteins of influenza A viruses using enzyme immunoassay]     

C Mohr  L D?hner  B Herrmann  H Herrmann 《Archiv fuer experimentelle veterinaermedizin》1978,32(4):495-500

Matrix protein is known as a type-specific structural protein of influenza viruses. An attempt has been made to find out whether or not strain-specific components could be detected from matrix protein, in addition to its type-specific antigen determinants. The technique of enzyme immune assay was chosen as the optional method to differentiate between matrix proteins of various influenza-A viruses. Antigen titration was undertaken of several matrix proteins, using two specific anti-matrix-protein sera in each case. Information regarding serological relationships between the tested matrix proteins of various influenza-A viruses was obtained from a quotient between the titres of one antigen, on the one hand, and the two anti-matrix-protein sera used in titration, on the other. Two matrix protein sub-types were established in the context of the influenza-A viruses tested. Sub-type M1 was attributable to older strains (A/PR/8 and A/FM/1), whereas the matrix protein of sub-type M2 was found to be present in more recent strains (A/Hongkong and A/Port Chalmers).  相似文献   

On the carcinomatous differentiation of dermatitis solaris chronica in cattle     

H J Herrmann  G Prietz 《Monatshefte für Veterin?rmedizin》1966,21(21):826-832

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Determining of nitrogen absorption and nitrogen secretion in different sections of the pig's intestine by digesta exchange between 15N labelled and unlabelled animals     

K Krawielitzki  T Zebrowska  R Schadereit  J Kowalczyk  U Hennig  J Wünsche  U Herrmann 《Archives of Animal Nutrition》1990,40(1-2):25-37

In an experiment with 3 pigs (initial live weight 30 kg, each fitted with 2 re-entrant fistulas in duodenum and ileum, one labelled with 15N), the duodenal and ileum digesta was exchanged. The N and 15N contents were estimated in faeces, urine, duodenal and ileum digesta of all experimental animals as well as in special organs and in the contents of different tract sections. The 15N excess (15N') of N compounds secreted into the gut lumen was determined using the 15N' in pancreas, gut mucosa and TCA-soluble blood serum. From measuring the digesta passage through the 3 sections of the digestive tract: 1. mouth ... duodenum, 2. duodenum ... ileum, 3. ileum ... after (Krawielitzki et al., 1989) the absorption and secretion rates of nitrogen were calculated. Secretion into the 1st section amounted to 5.3 g N/d (= 15% of intake) and the absorption to approximately 1% of intake. In the 2nd section the corresponding dates were 8.9 resp. 38.6 g N/d (= 25 resp. 110% of N intake), and in the 3rd one 1.9 resp. 8.4 g N/d (= 5.6 resp. 24% of N intake). Total absorption amounted to 134% of N intake and the over all re-absorption of endogenous N compounds secreted into the gut lumen to about 90%. During the passage the amount of endogenous N (g/d) decreased from 5.3 at the duodenum to 3.8 at the ileum to 1.6 in the faeces, but the relative portion increased (13 resp. 35 resp. 39%). An incorporation into body proteins occurred only from N compounds absorbed in the 1st and in the 2nd section. N (or 15N) absorbed in the large intestine was almost quantitatively excreted by urine. The method of digesta exchange between cannulated labelled and unlabelled pigs seems to be a suitable method to estimate absorption and secretion of exogenous and endogenous N portions in various sections of the digestive tract.  相似文献   

Die Eichelmastnutzung einst und jetzt     

Henrich Herrmann 《Forstwissenschaftliches Centralblatt》1915,37(2):51-60

Ohne Zusammenfassung  相似文献   

Determination of the transit rates in different sections of the pig's intestine     

K Krawielitzki  U Hennig  T Zebrowska  J Kowalczyk  U Herrmann 《Archives of Animal Nutrition》1990,40(1-2):17-23

An experiment was carried out using pigs weighing approximately 30 kg. The animals were fitted with two re-entrant cannulas in duodenum and ileum. During a 5 day period the passage of digesta through duodenum and ileum as well as the excretion by urine and faeces was estimated, taking an aliquot of 5% for N analysis. Transit of digesta amounted to 12.4 ... 13.2. kg/d in the duodenum and 2.7 ... 3.6 kg/d in the ileum. The appertaining N passage rates were 36.8 ... 42.4 resp. 8.7 ... 11.2 g N/d, corresponding to 108 ... 120% and 27 ... 32% of the N intake. The transit rate of duodenal digesta was highest immediately after feeding (1.4 ... 1.5 kg/h), decreased thereafter strongly, reaching a second lower maximum of 0.85 ... 1.0 kg/h 2 ... 3 h after feeding and then going down to 0.3 ... 0.4 kg/h just before the next feeding. The daily mean value was about 500 g/h. Endogenous N content of duodenal digesta varied between 10% after feeding and 50% 6 ... 12 h after feeding, with an average of 18.1%. In contrast the endogenous N content of ileal digesta was relatively constant amounting about 42% during the whole day. These findings correspond with those found by other authors using other rations and other live weights of the pigs. They refer to a clear diurnal rhythm of digesta transit and to the enormous dynamics of absorption and secretion processes in the intestinal tract of pigs during digestion.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Theoretical study of the effect of round straps on the selectivity in a diamond mesh cod-end     

Bent Herrmann  Daniel Priour  Ludvig A. Krag   《Fisheries Research》2006,80(2-3):148-157

FEMNET, a numerical tool based on the finite element method, was applied to estimate the shapes of various diamond-mesh cod-end designs during fishing. The only design differences rest in the use of round straps of different lengths, positions and numbers. The cod-end shape estimates were then entered in the selectivity simulation tool PRESEMO to simulate the selectivity processes of the various cod-end designs under the same varying fishing conditions. This enabled us to demonstrate how one or two round straps along the cod-end axis may change the selectivity of the cod-end compared with a reference cod-end, without round straps. We predict that in cod-end designs, which comply with the EU legislation, the 50% retention length (L50) may be reduced by up to 1.5 cm (5%) for haddock.  相似文献   

Biomass from landscape management of grassland used for biogas production: effects of harvest date and silage additives on feedstock quality and methane yield     

C. Herrmann  A. Prochnow  M. Heiermann  C. Idler 《Grass and Forage Science》2014,69(4):549-566

Biogas production from grassland biomass harvested during landscape management may help to maintain species‐rich grassland biotopes, but extensive management and late harvests often result in low‐quality biomass. Biogas production from the vegetation of Alopecuretum pratensis, Molinietum caeruleae and Caricetum gracilis, three typical grassland biotopes in north German nature reserves, was investigated in relation to harvest date. In addition, the A. pratensis vegetation was investigated for ensiling and the application of bacterial silage additives. Results indicate that biogas production might be a reasonable utilization pathway for grassland biomass from landscape management if the first cut occurs up to late summer. Methane yields of grassland biomass decreased substantially with later harvest, from up to 309 l_N kg^?1 organic dry matter (ODM) in May to below 60 l_N kg^?1 ODM in February, in correlation with increasing crude fibre contents. Caricetum gracilis vegetation was the least suitable feedstock for biogas production. It showed a rapid decline in methane yields with later harvest and 25% lower methane yields compared with other types of grassland vegetation. Application of silage additives is recommended for adequate preservation of grassland biomass from landscape management by ensiling. Addition of homofermentative lactic acid bacteria improved acidification during ensiling if sufficient fermentable sugar was available. The use of inoculant and molasses enhanced methane yields by 3–55%. Additional carbohydrate source is necessary to ensure proper ensilage when grasses are harvested after late autumn.  相似文献