Characterization of Ribulose-1,5-bisphosphate carboxylase-oxygenase activase (Rca) genes in durum wheat     

Nigro  Domenica  Giove  Stefania L.  Colasuonno  Pasqualina  de Pinto  Roberta  Marcotuli  Ilaria  Gadaleta  Agata 《Genetic Resources and Crop Evolution》2022,69(6):2191-2202

Genetic Resources and Crop Evolution - Durum wheat (T. turgidum L. var. durum) is one of the most widely cultivated cereal crop in the Mediterranean area. Its production has been triggered by...  相似文献   

TRAP molecular markers as a system for saturation of the genetic map of durum wheat     

Virginia Menzo  Angelica Giancaspro  Stefania Giove  Domenica Nigro  Silvana Zacheo  Pasqualina Colasuonno  Ilaria Marcotuli  Ornella Incerti  Antonio Blanco  Agata Gadaleta 《Euphytica》2013,194(2):151-160

Target region amplification polymorphism (TRAP) is a relatively new PCR-based technique that detects large numbers of loci in a single reaction without extensive pre-PCR processing of samples. The aim of this study was to integrate TRAP markers in an EST-derived SSR linkage map of a RIL mapping population from the cross of the durum wheat cultivars Ciccio and Svevo, for a more general purpose of establishing a high-throughput system for genetic map saturation. Primer combinations producing PCR products with at least 4–5 polymorphic bands were selected and analyzed across the mapping population. The PCR reactions produced a total of 2,881 fragments with an average of 52 peaks per reaction. A total of 142 new TRAP markers were mapped and found to be randomly distributed in the genome. The total length of the map was 2,043.0 cM, with an average chromosome length of 145.9 cM. Homoeologous group one had the highest number of TRAP markers (38 loci) and the longest map length (407.9 cM) for a total of 87 markers, while the homoeologous group five had the lowest TRAP marker number (5 loci) and the shortest map length (232.5 cM). The distribution of markers among the seven homoeologous groups was random. The results indicate that TRAP is highly efficient in genetic mapping, generating a large number of markers scattered across the genome. This closes many existing gaps in marker coverage and may join otherwise separate linkage groups.  相似文献