Biofilm bacteria: formation and comparative susceptibility to antibiotics   总被引：17，自引：0，他引：17       下载免费PDF全文

Merle E. Olson  Howard Ceri  Douglas W. Morck  Andre G. Buret    Ronald R. Read 《Canadian journal of veterinary research》2002,66(2):86-92

The Calgary Biofilm Device (CBD) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin G, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative bacteria were determined. Bacterial biofilms were readily formed on the CBD under selected conditions. The biofilms consisted of microcolonies encased in extracellular polysaccharide material. Biofilms composed of Arcanobacterium (Actinomyces) pyogenes, Staphylococcus aureus, Staphylococcus hyicus, Streptococcus agalactiae, Corynebacterium renale, or Corynebacterium pseudotuberculosis were not killed by the antibiotics tested but as planktonic bacteria they were sensitive at low concentrations. Biofilm and planktonic Streptococcus dysgalactiae and Streptococcus suis were sensitive to penicillin, ceftiofur, cloxacillin, ampicillin, and oxytetracycline. Planktonic Escherichia coli were sensitive to enrofloxacin, gentamicin, oxytetracycline and trimethoprim/ sulfadoxine. Enrofloxacin and gentamicin were the most effective antibiotics against E. coli growing as a biofilm. Salmonella spp. and Pseudomonas aeruginosa isolates growing as planktonic populations were sensitive to enrofloxacin, gentamicin, ampicillin, oxytetracycline, and trimethoprim/sulfadoxine, but as a biofilm, these bacteria were only sensitive to enrofloxacin. Planktonic and biofilm Pasteurella multocida and Mannheimia haemolytica had similar antibiotic sensitivity profiles and were sensitive to most of the antibiotics tested. The CBD provides a valuable new technology that can be used to select antibiotics that are able to kill bacteria growing as biofilms.  相似文献   

Diagnosis and outcome following tenoscopic surgery of the digital flexor tendon sheath in German sports and pleasure horses     

Andrea N. Cender  Kathrin Mählmann  Anna Ehrle  Roswita Merle  Laura Pieper  Christoph J. Lischer 《Equine veterinary journal》2023,55(1):48-58

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Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome     

Zientara S  Sailleau C  Dauphin G  Roquier C  Rémond EM  Lebreton F  Hammoumi S  Dubois E  Agier C  Merle G  Bréard E 《The Veterinary record》2002,150(19):598-601

In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.  相似文献   

Giardiasis and cryptosporidiosis in ruminants.     

Ryan M O'Handley  Merle E Olson 《Veterinary Clinics of North America: Food Animal Practice》2006,22(3):623-643

Although they differ considerably with respect to their biology, both Giardia duodenalis and Cryptosporidium parvum are common in ruminants, whereas Cryptosporidium andersoni is not. G. duodenalis infections are acquired during the first few months of life, tend to be chronic, and may be a production-limiting disease of ruminants. C. parvum infections remain an important cause of diarrhea in neonatal ruminants. Abomasal cryptosporidiosis, caused by C. andersoni, is an emerging disease of cattle that may affect both beef and dairy herds. This article reviews the life cycles, production impacts, treatments, controls, and zoonotic potentials of these important ruminant parasites.  相似文献   

Ammonium‐driven nitrification plays a key role in increasing Mn availability in calcareous soils     

Michael Dreyer  Merle Wichmann  Marie Rischen  Bruno M. Görlach  Annika Ehmke  Britta Pitann  Karl H. Mühling 《植物养料与土壤学杂志》2020,183(3):389-396

Background : Manganese deficiency often becomes a yield limiting factor, particularly on calcareous soils, even though the total soil manganese content is usually sufficient. Although it is known that acidifying N fertilizers can improve Mn availability, the reason of this effect is still unknown. Aim : Our aim was to investigate the effect of stabilized ammonium fertilizers as a tool to distinguish between physiological‐ and nitrification‐induced acidification. Method : Two pot experiments with Triticum aestivum L. and one soil incubation experiment using different nitrogen forms (CN = calcium nitrate, AN = ammonium nitrate, AS = ammonium sulfate, ATS = ammonium thiosulfate) with and without addition of nitrification inhibitors (DCD, Nitrapyrin, Piadin, DMPP) were conducted to examine the effect on Mn availability in the soil and Mn uptake by the plants at different development stages (EC 31 und 39). Results : With increasing fertilizer ${\mathrm{NH}}_4^{+}$ content a higher Mn concentration was detected: CN: 32 µg Mn g^?1 DM, AN: 39 µg Mn g^?1 DW, AS: 55 µg Mn g^?1 DM, ATS: 109 µg Mn g^?1 DM. The addition of a nitrification inhibitor resulted in a significantly lower rhizosphere pH compared to the non‐stabilized fertilizer. Surprisingly, the use of different nitrification inhibitors led to unchanged (CN, AN) or lower Mn concentrations of wheat. Especially in the ${\mathrm{NH}}_4^{+}$ treatments (AS and ATS), this negative effect was very evident (AS+DCD: 42 µg Mn g^?1 DM; ATS+DCD: 55 µg Mn g^?1 DM). Conclusions : Mn availability was enhanced by ongoing nitrification process rather than physiological acidification. Compared to other N forms, ammonium thiosulfate led to the highest Mn availability in bulk soil.  相似文献   

The ups and downs of global research centers     

Matthiessen CW  Schwarz AW  Find S 《Science (New York, N.Y.)》2002,297(5586):1476-1477

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Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release   总被引：1，自引：0，他引：1  

Kittel RJ  Wichmann C  Rasse TM  Fouquet W  Schmidt M  Schmid A  Wagh DA  Pawlu C  Kellner RR  Willig KI  Hell SW  Buchner E  Heckmann M  Sigrist SJ 《Science (New York, N.Y.)》2006,312(5776):1051-1054

The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.  相似文献   

Retrospective analysis after endoscopic urethral injections of glutaraldehyde‐cross‐linked‐collagen or dextranomer/hyaluronic acid copolymer in bitches with urinary incontinence     

K. Lüttmann  R. Merle  R. Nickel 《The Journal of small animal practice》2019,60(2):96-101

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Blepharitis in dogs: a clinical evaluation in 102 dogs     

Christiane Weingart  Barbara Kohn  Martina Siekierski  Roswitha Merle  Monika Linek 《Veterinary dermatology》2019,30(3):222-e69

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Influence of feeding and other factors on adrenocorticotropin concentration and thyrotropin-releasing hormone stimulation test in horses and ponies     

Karolina Drozdzewska  Judith Winter  Ann Kristin Barton  Roswitha Merle  Heidrun Gehlen 《Equine veterinary journal》2024,56(2):342-351

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