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Zoldoš Vlatka Vidaković-Cifrek Željka Tomić Mihovil Papeš Dražena 《Water, air, and soil pollution》1997,94(1-2):181-190
Allium test has already been used to determine cytotoxicity of waste drilling fluids. In the present work the cytotoxicity of four pure chemicals (Defoamex, Idthin 400, Magco Thin and Slick Pipe) was investigated. Those chemicals are components of drilling fluids, therefore, they are usually constituents of oil and gas industry waste waters. The tested chemicals were prepared in 1:9 dilution and cytotoxic effects on root-tip meristem ofAllium ascalonicum were analysed after 24-, 48- and 72-hour-treatments. All samples showed cytotoxicity which was proved by cytogenetic parameters such as inhibition of mitotic activity and increase of mitotic abnormalities and chromosomal aberrations in comparison with the control. Almost all mitotic abnormalities induced by four chemicals tested were the result of disturbed spindle mechanisms accompanied with stickiness. Slick Pipe revealed the most prominent mitodepressive effect and induced a high number of abnormalities. It caused a significant decrease of mitotic activity and increase of mitotic abnormalities after all three treatment durations, while chemicals Defoamex and Magco Thin showed a significant decrease of mitotic activity only after 72-hour-treatment. Chemicals Defoamex, Idthin 400 and Magco Thin revealed a significant increase of mitotic abnormalities after 48- and 72-hour-treatments. 相似文献
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The toxicity of four waste water samples from reserve pit upon the root-tip cells of Allium ascalonicum L. was researched by applying modified Allium test. The samples were a mixture of substances and their chemical composition was only partly known. The first task was to find out the optimal duration for root-tips treatment. The treatment lasting 48 h has been accepted because it enables delivery of results only four days after the sample has been received. All undiluted four waste drilling fluid samples showed cytotoxicity as well as toxicity, which was proved by cytogenetic parameters such as high inhibition of mitotic activity or low mitotic index and high percentage of chromosomal and genomic aberrations. The toxicity was significantly reduced by increasing the degree of sample dilutions. The results of this investigation demonstrated that the modified Allium test could be easily used for detection of waste drilling fluids toxicity degree. 相似文献
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