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This study compared the amino acid (AA) profile of five residues (original forage, borate-phosphate buffer residue (BPR), neutral detergent fiber residue with (NDF+) and without (NDF-) sodium sulfite, and acid detergent fiber residue (ADF). Fourteen grasses and legumes from tropical and temperate regions were used in this study. The use of sodium sulfite did not affect the NDF concentration, but the NDF insoluble protein was lower (P < 0.05) in the NDF+ than in the NDF- (3.9 vs 4.5% DM, respectively). For all of the amino acids tested, the amino acid content, expressed as a percentage of CP, was lower in the ADF residue than in the original forage. There were no differences in the amino acid concentrations of the NDF- and NDF+ extracts (P > 0.05). Only in the case of methionine was there a difference in the amount of amino acid when the original forage was compared with the BPR (1.84 vs 1.45 % CP). When the AA profile of each residue was corrected for the AA content of the ADF, no difference was observed between the AA profile of the original forage and of the BPR (P > 0.05). Similar to the result without correction for the amino acids in ADF, the AA profiles of the NDF+ and NDF- fractions were similar (P > 0.05). From this result, we infer that the sodium sulfite had similar effects on all AA in the NDF residue that we tested. There were differences in amino acid concentrations in the original forage and the NDF residues for several amino acids (Met, Cys, Lys, Thr, Arg, Ile, Leu, and Phe) (P < 0.05). When the amino acid values of the original forage and the BPR were used with animal data in the Cornell Net Carbohydrate and Protein System model, few differences in animal predicted performance were evident. These findings suggest that the AA profile of the original forage can be used to predict the AA profile of the undegraded intake protein instead of using the borate-phosphate buffer residue for amino acid analyses. This would simplify obtaining feed amino acid values for use in the Cornell Net Carbohydrate and Protein System.  相似文献   
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Lignin residues are available in large amounts as kraft lignin from chemical pulping processes. This lignin is mainly incinerated in recovery boilers. The recovery boilers are often the bottle-necks in the overall pulping process when pulp production increases are desired. Through cross-flow nano-filtration of the black liquor from kraft pulping, a low-molecular weight lignin fraction can be removed thus decreasing the organic load on the recovery boilers. The low-molecular weight lignin fraction furthermore exhibit different characteristics compared to other commercial kraft lignins and represents a new raw material source in novel applications.The low-molecular weight lignin was used together with a vegetable oil to produce a new hydrophobic lignin derivative similar to suberin. The lignin and the lignin derivative was analysed with FT-IR, UV–vis and SEC. The ability of the product to make paper surfaces hydrophobic was also evaluated.The results demonstrate the possibility to make a suberin-like lignin derivative that is potentially of interest in paper-coating applications due to its capability to interact well with wood fibres and make paper hydrophobic.  相似文献   
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Induction of discolored wood in Scots pine (Pinus sylvestris L.) trees by treatment with ethylene, carbon dioxide, nitrogen (hypoxia) or wounding from early April to late September was investigated. All treatments induced formation of discolored wood upward and downward from the drill hole. The amount of discolored wood formed above the drill hole depended on the treatment in the following order: ethylene > carbon dioxide = nitrogen > wounding; and below the drill hole in the order: ethylene > carbon dioxide = nitrogen = wounding. Based on chemical analyses (HPLC/UV, GS/MS, LC/MS and 1H-NMR), discolored wood induced by wounding or treatment with ethylene or carbon dioxide showed compositional similarities to natural heartwood, whereas discolored wood induced by nitrogen treatment showed fewer similarities to natural heartwood. The results suggest that ethylene is an important factor controlling heartwood formation, although wounding and internal concentrations of carbon dioxide may also play a role.  相似文献   
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Abstract – Fish, which are generally visual foragers, experiences reduced reaction distance in visually degraded environments, which has consequences for encounter rates with prey. Small prey is detected at shorter distances than larger prey, and piscivores are therefore predicted to be more strongly affected by visual degradation. In experiments, roach (Rutilus rutilus) were fed two plankton prey types and pike (Esox lucius) were fed Daphnia and larval roach, in clear water, algal turbid water and water coloured brown by dissolved organic matter (DOM). Planktivorous foraging in roach was not affected by visual degradation, while pike foraging on both Daphnia and larval roach was. Pike showed increased reaction distance to Daphnia in visually degraded water, while it was severely reduced with roach as prey even if the visual range was not reduced below pike reaction distances in clear water. Pike foraging on Daphnia was not affected, but when foraging on roach, the reduced search efficiency was counteracted by increased attack rates. However, there was no increase in movement and no difference between turbid and DOM treatments. Effects on piscivores will likely become more pronounced at later life stages as prey size and the reliance on long‐distance detection increases at the same time as changing climatic conditions may further deteriorate the visual conditions in future.  相似文献   
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Ceramide triggers budding of exosome vesicles into multivesicular endosomes   总被引:5,自引:0,他引:5  
Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.  相似文献   
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Globally, aquaculture systems with water recirculation experience increasing problems with microbial taste and odor compounds (TOCs) such as geosmin and 2-methylisoborneol (MIB). This study investigated the content of geosmin and MIB in water and the flesh of 200 rainbow trouts from eight recirculated aquaculture systems in Denmark. TOC content in the fish flesh was measured by a dynamic headspace extraction method and was evaluated by a sensory panel. The results showed significant correlations between TOC content in water and fish and between chemical analysis and sensory perception. When geosmin exceeded 20 ng/L in the water, 96% of the fish had an intense muddy flavor, but below 10 ng geosmin/L, 18% of the fish (only 3% in special depuration ponds) had an intense muddy flavor. The results indicate that TOC levels <10 ng/L will ensure that a negligible portion of the fish obtains an unpalatable taste and flavor due to TOCs.  相似文献   
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In Sweden, 90% of ammonia (NH3) emissions to the atmosphere originate from agriculture, predominantly from animal manure handling. It is well known that incorporation of manure into soil can reduce NH3 emissions after spreading. However, there is a risk of increased nitrous oxide (N2O) and methane (CH4) emissions caused by bacterial activity and limited oxygen availability under these conditions. A full‐scale injector was developed and evaluated in a field experiment on grassland. Cattle slurry was either injected in closed slots 5 cm below ground or band spread on the soil surface above the crop canopy at a rate of 25 t ha?1. In a control treatment, no slurry was applied. During a 5‐day period after application, NH3 emissions were measured using an equilibrium concentration method. Gas samples for estimating CH4 and N2O emissions were also collected during 7 weeks following slurry application. Injection in closed slots resulted in no detectable NH3 emissions. After band spreading, however, NH3 emissions corresponded to nearly 40% of the total ammoniacal nitrogen in the applied slurry. The injection of slurry gave rise to a broad peak of N2O emissions during the first 3 weeks after application. In total, for the measuring period, N2O emissions corresponded to 0.75 kg N ha?1. Band spreading resulted in only a very small N2O release of about 0.2 kg N ha?1 during the same period. Except for the first sampling occasion, the soil was predominantly a sink for CH4 in all the treatments. The use of the injector without slurry application reduced grass yield during unfavourable growing conditions. In conclusion, shallow injection in closed slots seems to be a promising technique to reduce negative environmental impacts from NH3 emissions with a limited release of N2O and CH4.  相似文献   
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