多胞质、多核背景下玉米细胞质雄性不育系及其保持系的同工酶分析   总被引：1，自引：0，他引：1  

曹墨菊  荣廷昭 《四川农业大学学报》1998,16(4)

对玉米同核异质和同质异核两套不育系及其相应保持系单核至双核期的花药,采用薄层垂直平板聚丙烯酰胺凝胶不连续电泳及光密度扫描方法进行酯酶和过氧化物酶同工酶分析。结果表明：这两种酶的同工酶在同一核背景下的不胞质之间和同一胞质下的不同核背景之间均表现出不同程度的差异,各类不育系与其相应保持系之间也表现出一定程度的差异。结合试验结果文中还对质核互作雄性不育产生的机理进行了讨论。  相似文献   

犬4种皮肤病的诊治     

霍元  付立伟  周墨菊 《北京农业》2010,(24)

北京市密云县某社区统计饲养宠物犬约375只,其中约200只能够得到精心饲养,定期进行洗浴。调查发现,在定期洗浴的所有犬中,仅有少部分患有轻微皮肤病,个别患病严重;未定期洗浴的犬中,患皮肤病的现象十分普遍,并且相当一部分病情严重。在动物疫病预防控制中心就诊的23个病例中,犬被螨虫感染或真菌感染的概率都很高,分别为10/23、8/23,比例相当。犬同时被2者感染的概率较低,为4/23。其中,雄性患病率13/23;雌性患病率10/23,并未发现犬的皮肤病与性别有关。经过用药治疗,在2周内康复的病例为3例,第3周康复的为7例,4周以后康复病例较多,但其中1例继发感染,死于败血症。  相似文献   

Identification and genetic analysis of two maize CMS-T mutants obtained from out-space-flighted seeds     

Yi  Hongyang  Zhang  Caibo  Li  Chuan  Wang  Jing  Yu  Tao  Liu  Yongming  Cao  Moju 《Genetic Resources and Crop Evolution》2021,68(5):1937-1947

Male sterility is widely utilized for hybrid seed production. In this study, two new found male sterile mutants SauS4 and SauS5 were obtained from space flighted seeds of maize inbred line RP125. Then, genetic analysis, molecular markers identification, and cytological observation were conducted to confirm their male sterile types. For genetic analysis, the above two male sterile mutants were continuously backcrossed with two maize inbred line 18Hong and RP125, and four stable male sterile lines SauS4(18Hong), SauS5(18Hong), SauS4(RP125), SauS5(RP125) were generated by six-generation backcross. Restoring and maintaining relationship analysis showed that both Hui313 and Zifeng1 didn’t rescue the male sterility SauS4(18Hong) and SauS5(18Hong). Using CMS mitochondria-specific primers for PCR detection suggested that only a 440 bp band unique to CMS-T type was amplified in SauS4(18Hong), SauS5(18Hong), SauS4(RP125), and SauS5(RP125). Sequencing results showed that these bands sequences were identical in DNA level which compared with T-urf13. Cytological observations showed that the main abortion stages of SauS4 and SauS5 were at the middle stage of uninucleate microspores under the two nuclear backgrounds of 18Hong and RP125, exhibiting the characteristics of sporophyte sterility. All the above results pointed out the two male sterile mutants SauS4 and SauS5 belonged to the CMS-T type. Interestingly, some mitochondrial genome difference between SauS4(RP125) and SauS5(RP125) were revealed by AFLP analysis.

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高致病性禽流感免疫抗体对比分析     

李阳  周墨菊  张美晶 《北京农业》2011,(Z1):64-66

为加强高致病性禽流感的防控工作,抽检北京市密云县220份鸡血样进行免疫抗体监测,对结果进行对比分析。实验表明,免疫效果良好,抗体水平较高,通过抗体滴度、平均抗体滴度和标准差的对比可以看出规模场户免疫效果比散养户要好。  相似文献   

多胞质,多核背景下玉米细胞质雄性不育系及其保持系的同工？…   总被引：2，自引：1，他引：1  

曹墨菊  荣廷昭 《四川农业大学学报》1998,16(4):407-413

对玉米同核异质和同质异核两套不育系及其相应保持系单核至双核期的花药,采用薄层垂直平板聚丙烯酰胺凝胶不连续电泳及光密度扫描方法进行酯酶和过氧化物酶同工酶分析。结果表明：这两种酶的同工酶在同一胞质下的不同核背景之间均表现出不同程度的差异,各类不育系与其相应保持系之间也表现出一定程度的差异。结合试验结果文中还对质核互作雄性不育生产的机理进行了讨论。  相似文献   

空间条件对玉米自交系S37的诱变效应   总被引：11，自引：1，他引：11  

曹墨菊  荣廷昭 《中国农学通报》2001,17(1):1-3

利用返回式实验卫星搭栽玉米自交系S37的种子，通过遗传交配设计对其后代株系进行农艺性状的配合力分析。结果表明，空间条件可以改变纯系材料后代株系的配合力，并就数量性状的诱发突变及其表现特点进行了分析。  相似文献   

DREB基因双T-DNA植物表达载体的构建及验证   总被引：5，自引：0，他引：5  

韩兆雪  曹墨菊  朱祯  荣廷昭 《分子植物育种》2004,2(1):7-12

W34基因经pGEM-T-EASY载体,克隆到pBDREB载体EcoRI位点,得到在Amp平板上生长的转化子pBW34-DREB质粒;质粒pBW34-DREB、pSPROK、pBlueks分别经Pst I／KpnI、KpnI／EcoRI、PstI／EcoRI双酶切,三片段连接构建成中间表达载体pBWD-Tnos;再用SmaI／EcoRV双酶切pBWD-Tnos。将回收的DREB基因的完整的表达结构元插入到经SmaI酶切的质粒载体pCDMAR-Hyg中,构建成双T-DNA植物表达载体pCDMAR-pWDT-Hyg,大小为13．59kb,经酶切鉴定,证明构建的载体与预期设计的一致。将此双T载体用农杆菌介导法转化玉米胚性愈伤组织,期望得到无选择标记的安全的转基因植物。  相似文献   

Genetic variation for maize root architecture in response to drought stress at the seedling stage     

Rongyao Li  Yijin Zeng  Jie Xu  Qi Wang  Fengkai Wu  Moju Cao  Hai Lan  Yaxi Liu  Yanli Lu 《Breeding Science》2015,65(4):298-307

Although the root system is indispensable for absorption of nutrients and water, it is poorly studied in maize owing to the difficulties of direct measurement of roots. Here, 103 maize lines were used to compare root architectures under well-watered and water-stressed conditions. Significant genetic variation, with medium to high heritability and significant correlations, was observed for root traits. Total root length (TRL) and total root surface area (TSA) had high phenotypical diversity, and TRL was positively correlated with TSA, root volume, and root forks. The first two principal components explained 94.01% and 91.15% of total root variation in well-watered and water-stressed conditions, respectively. Thus, TRL and TSA, major contributors to root variation, can be used as favorable selection criteria at the seedling stage. We found that stiff stalk and non-stiff stalk groups (temperate backgrounds) showed relatively higher mean values for root morphological diversity than the TST group (tropical/subtropical background). Of the tested lines, 7, 42, 45, and 9 were classified as drought sensitive, moderately sensitive, moderately drought tolerant, and highly drought tolerant, respectively. Seven of the 9 extremely drought tolerant lines were from the TST group, suggesting that TST germplasms harbor valuable genetic resources for drought tolerance that could be used in breeding to improve abiotic stress tolerance in maize.  相似文献   

进一步做好布病净化工作     

付立娜  周墨菊  付立伟 《北京农业》2011,(Z1):34-35

针对北京市密云地区肉牛、羊、猪、犬布病的流行情况进行调查,2009-2010年抽样检测14个乡镇的41个养殖(场)户1万1803份样品,除奶牛免疫了布病疫苗未检测外,其余检测结果均为阴性。因为全县对布病的检疫净化工作的高度重视,所以2007-2010这4年里未发生肉牛、羊、犬、猪布病的疫情,进一步做好布病的检疫净化工作仍然是工作的重中之重。  相似文献   

玉米雄性不育系的质、核对农艺性状的影响   总被引：2，自引：2，他引：2  

曹墨菊  荣廷昭 《四川农业大学学报》1997,15(4):440-444

选用一组同核异质、四组同质异核不育系及保持系分别与测验系组成不完全双列杂交。对测交Ｆ１的吐丝期、株高、穗粒重等农艺性状进行了考察分析。结果表明：①同核异质组呈现出不同程度的胞质效应；同质异核组不同核背景之间表现出的差异较为明显。大多数性状的核基因组变异大于胞质基因组变异。②不育系在对农艺性状的影响上存在有质核互作效应。  相似文献