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Two extremely late heading mutants were induced by ion beam irradiation in rice cultivar ‘Taichung 65’: KGM26 and KGM27. The F2 populations from the cross between the two mutants and Taichung 65 showed clear 3 early: 1 late segregation, suggesting control of late heading by a recessive gene. The genes identified in KGM26 and KGM27 were respectively designated as FLT1 and FLT2. The two genes were mapped using the crosses between the two mutants and an Indica cultivar ‘Kasalath’. FLT1 was located on the distal end of the short arm of chromosome 8. FLT2 was located around the centromere of chromosome 9. FLT1 might share the same locus as EHD3 because their chromosomal location is overlapping. FLT2 is inferred to be a new gene because no gene with a comparable effect to that of this gene was mapped near the centromere of chromosome 9. In crosses with Kasalath, homozygotes of late heading mutant genes showed a large variation of days to heading, suggesting that other genes affected late heading mutant genes.  相似文献   
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The effects of NaCl stress on plant growth, gas-exchange, activity of superoxide dismutase (SOD), rate of lipid peroxidation, and accumulation of Na+ ion and sugar were investigated in leaves and fruits of pepper plants (Capsicum annuum L.). Especially, the gene expression of l-galactono-1,4-lactone dehydrogenase (GalLDH), which is the last enzyme of ascorbic acid (AsA) biosynthesis, and the relationships between AsA level and Na+ concentration in plant tissue were investigated with increasing salinity. Plants were treated with three treatments: the control (0 mM NaCl) and two salinity levels (50 and 100 mM NaCl) for 21 days under greenhouse conditions. Plant growth was markedly restricted due to the reduction of photosynthetic rate and the increase of Na+ accumulation in leaves with the increasing intensity of NaCl stress. Salinity had more effect on fruit growth comparing to leaf growth, suggesting that fruits could be more sensitive to salinity than leaves. In comparison with the control, salt stress significantly increased lipid peroxidation (as measured as malondialdehyde content) but decreased SOD activity in both fruits and leaves although the effect was larger in fruits; and the rate of the decrease in SOD activity was greater than that of the increase in lipid peroxidation. The AsA concentration transiently increased first 7 days but it slightly decreased from the initial level in the end of treatment day 21. The change in GalLDH gene expression was similar to AsA concentration. The accumulation of Na+, the reduction of AsA level at severe salinity stress were greater in fruits than in leaves; and AsA level had a negative relationship with Na+ concentration in both leaves and fruits. These results suggest that the difference in salt sensitivity between fruits and leaves in pepper plants can be related to the difference in inhibition of AsA synthesis, which in turn is probably due to the toxicity of extreme accumulation of Na+.  相似文献   
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Hybrid weakness is a reproductive barrier. In rice, the hybrid weakness caused by two complementary genes––HWC1 and HWC2––has been surveyed extensively. However, their gene products and the molecular mechanism that causes hybrid weakness have remained unknown. We first performed fine mapping of HWC2, narrowing down the area of interest to 19 kb. We thereby identified five candidate genes. Second, we performed haplotype analysis around the HWC2 locus of 33 cultivars. With 15 DNA markers examined, all the 13 Hwc2-1 carriers share the same haplotype for consecutive 14 DNA markers. As for hwc2-2 carriers, five out of 20 have the haplotypes relatively similar to those of Hwc2-1 carriers. However, the other haplotypes differ remarkably from them. These results are useful to identify the HWC2 gene and to study rice varietal differentiation.  相似文献   
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We studied the age and growth of the lizardfish Saurida elongata using ground thin sections of otoliths (sagittae) from specimens collected in the Tsushima/Korea Strait between May 1999 and June 2001. A total of 695 individuals with fork lengths (FL) ranging from 189 to 478 mm were examined. The frequency of translucent zone occurrence at the outer margin of the otoliths indicated that translucent zones formed once a year between November and February. Most of the males examined were estimated to be 2–7 years old and the females, 3–9 years old. The maximum estimated age of a male specimen was 10 years and that of a female, 11 years. The estimated von Bertalanffy growth curves were FL t  = 451{1 − exp[−0.172(t + 2.50)]} and FL t  = 515{1 − exp[−0.151(t + 2.47)]} for males and females, respectively. At all ages, the FL at a specific age for females was greater than that for males, suggesting that females of this species grow faster than males.  相似文献   
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用甘薯品种农林17号(中紫)的叶柄分离原生质体,对其在添加各种浓度的萘乙酸和激动素的改良细胞薄层培养基中进行悬浮培养。培养1~2 d 后,原生质体再生细胞壁,培养2~3 d 后,再生细胞发生第一次分裂,持续的细胞分裂形成小愈伤组织。悬浮培养的结果表明萘乙酸和激动素的适宜浓度分别为2.0~5.0mg/L 和1.0~5.0mg/L。此后,将小愈伤组织转移到MS 固体培养基上培养,小愈伤组织迅速生长。本试验达到根的分化.  相似文献   
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