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1.
M. Akhlasur Rahman Isaac Kofi Bimpong J. B. Bizimana Evangeline D. Pascual Marydee Arceta B. P. Mallikarjuna Swamy Faty Diaw M. Sazzadur Rahman R. K. Singh 《Rice》2017,10(1):47
Background
Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.Results
The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.Conclusions
The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.2.
Most often a genetic linkage map is prepared using populations obtained from two highly diverse genotypes.However the markers from such a map may not be useful in a breeding program as these markers may not be polymorphie among the varieties used in breeding.…… 《分子植物育种》2007,5(2):219-220
Most often a genetic linkage map is prepared using populations obtained from two highly diverse genotypes. However, the markers from such a map may not be useful in a breeding program as these markers may not be polymorphic among the varieties used in breeding. For the past nine years, intraspecific maps have been gaining importance and such maps based on Swiss (PaiUard et al., 2003), Japanese (Suenaga et al., 2005), Australian (Chaimcrs et al., 2001) wheat varieties arc available. A map based on Indian wheat varieties however has not been reported. We constructed a genetic linkage map based on a cross between two Indian bread wheat (Triticum aestivum L.) varieties, Sonalika and Kalyansona. One hundred and fifty F2 individuals were analyzed for arbitrarilyprimed polymerase Chain reaction (AP-PCR), random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR), Sequence Tagged Microsatelhte Sites (STMS), Amplified Fragment Length Polymorphism (AFLP) markers, seed storage proteins and known genes. A linkage map was constructed consisting of 236 markers and spanning a distance of 3 639 cM with 1 211.2 cM for A genome, 1 669.2 cM for B genome, 192.4 cM for D genome and 566.2 cM for unassigned groups, 相似文献
3.
Policegoudra RS Kumar MH Aradhya MS 《Journal of agricultural and food chemistry》2007,55(20):8105-8111
Accumulation of bioactive compounds and storage components during developmental stages of mango ginger ( Curcuma amada Roxb.) rhizome was investigated from 60 to 240 days, as a function of physiological maturity. Four distinct developmental phases were defined, namely, vegetative phase (up to 60 days from planting), initiation and development phase (60-150 days), maturation phase (150-180 days), and senescence phase (180 days). Difurocumenonol, a bioactive terpenoid compound and phenolics were identified as biomarkers, to determine the optimum physiological maturity to harvest mango ginger rhizome. Accumulation of phenolics was observed in newly initiated rhizomes (after 60 days from planting). The phenolic content was high in mango ginger pulp compared to its juice. Newly initiated rhizome contained no difurocumenonol, and it was observed after 120 days after planting. Peak accumulation of phenolics, difurocumenonol, and total protein were noticed in 180 day old rhizome. Accordingly, the abundance of these components on 180 days was set as an optimum maturity standard for harvest of mango ginger rhizome, compared with a conventional harvest period that ranges from 200 to 240 days. 相似文献
4.
There are many wild species of pigeonpea which are endemic to Australia. These wild species are cross incompatible with cultivated
species of Indian origin. Cajanus acutifolius is one such species which does not easily cross with cultivated pigeonpea. Interspecific pollinations lead to hybrid seeds
which were semi-shrivelled. Very few seeds germinated to give rise to F1 plants. Backcrossing the hybrid plants to C. cajan,
the male parent, gave rise to aborting seeds which did not germinate in vivo hence BC1 plants are obtained after saving the
aborting embryos in vitro. BC1 plants showed normal meiotic pairing, but had low pollen fertility. The reasons for embryo abortion and low pollen fertility
in spite of normal meiosis could be due to the effect of wild species cytoplasm.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
5.
Arachis glabrata Benth, variety glabrata collector GK 10596 (PI276233; ICG 8176) belonging to section Rhizomatosae has resistance to rust, late leaf spot and viral diseases. A. hypogaea L. cv MK 374 (section Arachis) is susceptible to rust, late leaf spot and to the viral diseases peanut stripe, peanut mottle and peanut bud necrosis. Hybrids
between A. hypogaea cv MK 374 and A. glabrata were produced after inter specific pollinations and embryo culture. The hybrids produced had morphological characters of
both parents plus floral abnormalities not seen in either parent. It was possible to identify the hybrids by esterase isozyme
analysis when still in culture. Cytological research showed variable chromosome association and also homeology between the
genomes of A. hypogaea and A. glabrata. The hybrids inherited resistance to rust, late leaf spot, peanut bud necrosis and peanut stripe diseases from the pollen
parent A. glabrata.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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An interspecific hybrid between Cicer arietinum cv. GL 769 and a wild species C. pinnatifidum was obtained after emasculation, pollination and application of growth regulators. Ovules were cultured and embryos were later dissected to obtain hybrid plants. These plants were albinos and morphologically resembled C. pinnatifidum. Shrivelled seeds were also obtained in 2% of the crosses, which on germination gave rise to albino plants. These plants did not survive beyond 20 days. The hybrid nature of these plants was confirmed by esterase isozyme studies. Hybrid shoots obtained from germinating embryos were cultured on modified ML-6 medium with BAP 2 mg/1, IAA 0.5 mg/1, where they turned green after 3–4 weeks. Transmission electron microscopy (TEM) studies on leaf sections from green hybrid shoots showed an improvement in the chloroplast structure, with better organized grana. 相似文献
8.
Epiphyte mats (contiguous pieces of live and dead epiphytes perched upon branches of trees) are a conspicuous component of tropical cloud forests and harbor diverse meso- and microarthropod communities. We investigated differences in arthropod assemblage structure between the vegetative (green) and humic (brown) portions of epiphyte mats in a lower montane forest in Monteverde, Costa Rica. Because of qualitative differences between the two substrates, we hypothesized that they would support different arthropod communities and that variation in community parameters would be linked to the quantity of brown material present in a mat sample. The green fraction contained twice as many individuals and species per gram dry mass than the brown fraction. Morphospecies composition was very similar between green and brown portions, but the relative abundance of several taxa differed significantly between the substrates. Contrary to our prediction, total arthropod abundance and richness in a sample were not correlated with the proportion of brown material present. In laboratory trials, the most common morphospecies of oribatid mite in this system showed a preference for brown substrates. 相似文献
9.
Nalini Mallikarjuna Deepak R. Jadhav Kanaka Reddy Fatema Husain Kumkum Das 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(1):17-21
Cultivated groundnut is susceptible to late leaf spot (LLS) caused by Phaeoisariopsis personata [(Berk. & M.A. Curtis) Aex] and resistance is low to moderate in the primary gene pool of groundnut. Closely related wild
species in the secondary gene pool are highly resistant to the disease. All the closely related Arachis species are diploid and cultivated groundnut is a tetraploid. Utilization of diploid Arachis species to transfer LLS resistance is time consuming and cumbersome. New sources of Arachis hypogaea (also called synthetic groundnut) were developed at ICRISAT. These are tetraploids and the present investigation has shown
that they are resistant to LLS. 相似文献
10.