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1.
Maryam Ahmadi Narges Damavandi Mohammad Reza Akbari Fatemeh Davami 《Iranian Biomedical Journal》2016,20(2):68-76
Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells; therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons. 相似文献
2.
Moslemizade N Rafiei A Yazdani F Hosseini-khah Z Yusefnezhad K 《Pakistan journal of biological sciences: PJBS》2011,14(2):106-111
Preeclampsia is a disease regarding with altered vascular reactivity leading to hypertension of the mother and metabolic alterations in the fetus. This study aimed to assess nitric oxide and bleeding time following administration of magnesium sulfate to preeclamtic patients compared to normotensive pregnant women. A total of 112 subjects (56 preeclamtic patients and 56 normotensive pregnant controls) were enrolled in this case-control study. Cases and controls were matched for age, BMI, gestational age, parity and gravidity. Total concentration of nitrite and nitrate (NOx) was measured before and during magnesium sulfate (MgSO4) treatment using a modified Griess-based method. RESULTS: Systolic and diastolic blood pressures were significantly decreased during MgSO4 treatment in preeclamtic patients (p < 0.0001). NOx levels were significantly increased in preeclamtic women after MgSO4 administration (33.7 +/- 18.5 vs. 50.2 +/- 21.6, p < 0.0001) but it was not seen in normotensive parturients (52.4 +/- 28.9 vs. 57.3 +/- 21.7, p = 0.362). The bleeding time was scarcely increased following magnesium sulfate treatment in preeclamptic patients compared to normotensive pregnant women but it was not significant (p = 0.18). In addition, there was only a significantly reverse correlation between NOx levels and systolic or diastolic blood pressure in preeclamtic parturients after MgSO4 treatment (r = -0384; p = 0.003 and r = -0.29; p = 0.03, respectively). This study demonstrates that administrating MgSO4 to preeclamtic patients induced significant changes in NOx production which had a major role in modulating vasculature changes in preeclamsia. 相似文献
3.
Mehrab Faraji Saeid Karimi Dehkordi Abdol Karim Zamiani Moghadam Behnam Ahmadipour Fariborz Khajali 《Journal of animal physiology and animal nutrition》2019,103(1):162-169
High levels of guanidinoacetate acid (GAA) deteriorate growth response in broiler chickens. We propose using coenzyme Q10, an antioxidant, and taurine (TAU), a methyl donor, to cope with the situation when high level of GAA included in diet. GAA was supplemented at 0 (control), 0.75, 1.5 and 2.25 g/kg in isoenergetic and isonitrogenous diets and fed to broilers (Cobb 500) from 1 to 40 days post‐hatch. Three additional diets were prepared by adding CoQ10 (40 mg/kg), TAU (40 mg/kg) or their combination (both CoQ10 and TAU at 40 mg/kg) to the 2.25 g/kg GAA group. The experimental design used was a completely randomized design. While weight gain (p = 0.038) and feed conversion ratio (p = 0.024) improved when GAA added at 1.5 g/kg, higher supplementation (2.25 g/kg) deteriorated these responses. These responses, however, were significantly restored by using CoQ10, TAU or their combination. Abdominal fat deposition was significantly decreased when TAU added to broiler diets by virtue of upregulating peroxisome proliferator‐activated receptor alpha. Supplementing broiler diets with CoQ10 and TAU or their combination significantly decrease ascites mortality. In conclusion, CoQ10 and TAU have shown beneficial effects when high level of GAA included in broiler diets. 相似文献
4.
Mehrab Yadegari 《Communications in Soil Science and Plant Analysis》2017,48(3):307-315
To evaluate the effects of irrigation period and iron (Fe), zinc (Zn) foliar application on agronomic characters in Borago officinalis, Calendula officinalis, Thymus vulgaris and Alyssum desertorum, investigations were performed in a complete randomized block design with three replications in 2014 and 2015. The factors applied were (a) Fe foliar application (0, 200, 400, 600 ppm), (b) Zn foliar application (0, 200, 400, 600 ppm) and (c) irrigation periods (3, 6, 9 days). Results showed that Fe and Zn had a significant effect on many factors including shoot dry matter, height, flower production (marigold, borage and thyme) and seed (alyssum). Interaction of 400 ppm of Zn and Fe with irrigation period every 3 days was the best and produced the best amounts in most of the measured characters. The lowest of the measured characters was 600 ppm of Fe and Zn by irrigation period of 9 days. Results showed that Zn micronutrient was more effective than Fe. 相似文献
5.
Behrouz Golhasan Mehrab Esmaeili Yiwu Fang Joaquín Abolafia Ramin Heydari 《Forest Pathology》2019,49(6)
Ektaphelenchoides maafiae n. sp. was isolated during a survey of nematodes associated with bark samples of an oak tree (Quercus castaneifolia) in Gorgan, Golestan Province, northern Iran. The new species has a body length of 480–546 μm (in females) and 431–480 μm (in males). The cuticle is weakly annulated, with three lateral lines. Lip region offset. The stylet is 13–15 μm long without basal swellings. The excretory pore is located at the level of the metacorpus base to slightly posterior, and hemizonid is at 15–17 μm posterior to the excretory pore. The post‐uterine sac is short, 6–8 μm long. Spicules having rounded condylus, rostrum short, conical with bluntly pointed tip, a cucullus (apophysis) presented on the dorsal distal end. Male tail bearing four (2 + 2) caudal papillae, conical, with sharply pointed terminus. The new species is close to four known species of the genus, including E. hunti, E. ruehmi, E. caspiensis and E. poinari, but differs from them by body size, shape of tail terminus, stylet length, shape and size of spicules, length of post‐vulval uterine sac and number of caudal papillae. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rDNA confirmed its status as a new species. 相似文献
6.
Gaucher's disease is a rare lipid storage disorder, affecting one in 40,000-200,000 people and results from a genetic deficiency of the enzyme glucocerebrosidase (glucosylceramidase). We report a 10-year old Iranian girl with chief complaint of anemia from 8 years ago, managed for iron deficiency anemia. The patient had hepatomegaly associated with huge splenomegaly which was confirmed by sonography. No skeletal disorder was found. Bone marrow aspiration revealed typical Gaucher cells. Low level of beta-glucocerebrosidase enzyme activity confirmed the Gaucher disease. The patient is now under treatment with CEREZIME, a recombinant DNA modified form of glucocerebrosidase with good condition. 相似文献
7.
Ghorbani B Vahdani N Zerehdaran S 《Pakistan journal of biological sciences: PJBS》2008,11(12):1582-1587
To investigate the effects of niacin supplementation in the diet of high producing cows at early lactation, 21 holstein dairy cows were used in this experiment. Animal were assigned in to three groups based on their milk yield and calving date soon after parturition. They were received a basal diet and 0 (group 1), 6 (group 2), 12 (group 3) g of supplementation niacin per day over a 10 weeks experimental period. Milk volume was recorded and milk samples were collected for each cow at two weeks interval for analysis of fat, protein, lactose and SNF (Solid-None Fat). Blood samples were also taken for the measurement of glucose, triglyceride, Beta-hydroxy butyrate and total protein at two weeks intervals. No significant difference were observed between milk yield, milk fat, protein, lactose and SNF content in cows received niacin compared to the control group (p > 0.05). Plasma glucose in groups 2 and 3 compared to the control were higher and this difference were statistically significant (p < 0.05). Blood triglycerides were not significantly affected by niacin supplementation. BHBA were lower in cows received niacin and this difference were significant (p < 0.05). The trend of changes in the amount of blood total protein were identical in all three groups whole the level of this factor was always higher in control group compared to the others groups. Niacin has showed an increase in the level of plasma glucose and a notable decrease in the amount of blood triglyceride, beta-hydroxy butyrate and total protein, which may be due to the effect of this vitamin on the energy metabolism in cows. 相似文献
8.
Effects of Dietary Ascorbic Acid on Growth Performance,Body Composition,and Some Immunological Parameters of Caspian Brown Trout,Salmo trutta caspius 
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下载免费PDF全文 A 9‐wk feeding experiment was conducted to investigate the effect of dietary ascorbic acid (AA) on growth performance and tissue compositions of Caspian brown trout. The fish (9.64 ± 0.62 g) were held in tanks at a density of 40 fish per tank. Five experimental diets were prepared by providing of 9.8, 43.8, 89.4, 188.5, and 384.2 mg AA equivalent/kg diet, respectively. At the end of the trial, an increasing trend was found on growth performance with the increase of AA supplementation. Fish fed diet supplemented with more than 188.5 mg/kg AA also had significantly higher bone collagen than fish fed diets containing 9.8 mg/kg AA (P < 0.05). According to the broken line, the dietary AA requirement of Caspian brown trout fingerlings was 177.24 mg AA/kg diet based on the weight gain, 186.97 mg AA/kg diet based on the protein tissue, and 163.95 mg AA/kg diet based on the bone collagen. Serum lysozyme and alternative complement pathway activities were also influenced by graded level of AA supplementation to the diet. The present research clearly indicated that AA is required for normal growth and enhancement of immune response in Caspian brown trout fingerlings. 相似文献
9.
Nayereh Nouri Narges Nouri Omid Aryani Behnam Kamalidehghan Maryam Sedghi Massoud Houshmand 《Iranian Biomedical Journal》2012,16(4):223-225
Background
Ataxia with oculomotor apraxia type 1 (AOA1) shows early onset with autosomal recessive inheritance and is caused by a mutation in the aprataxin (APTX) gene encoding for the APTX protein.Methods
In this study, a 7-year-old girl born of a first-cousin consanguineous marriage was described with early-onset progressive ataxia and AOA, with increased cholesterol concentration and decreased albumin concentration in serum. PCR and direct DNA sequencing was performed after DNA extraction.Results
Sequencing analysis revealed a novel homozygous deletion in c.643 and A>T single nucleotide polymorphism in c.641 in exon 6 of the APTX gene [ENST00000379825].Conclusion
It seems that this region of exon 6 is probably a hot spot; however, no deletions have been reported in exon 6 yet. Key Words: Ataxia oculomotor apraxia 1 (AOA1), aprataxin (APTX), Iranian 相似文献10.
Mehrab Esmaeili Ramin Heydari Mozhgan Ziaie Weimin Ye 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(3):533-542
A new species in the genus Ditylenchus, D. stenurus n. sp. collected from western Iran, is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 772 (663–863) μm, delicate stylet 6 (5–7) μm long, six lines in the lateral field. Median bulb of pharynx well-developed, muscular with crescentic valve. Post-vulval uterine sac well-developed, 35 (30–45) μm long, female tail elongate-conoid, becoming narrow suddenly with finely rounded terminus. The new species comes close in morphology and morphometrics to five known species of the genus, namely D. arachis, D. caudatus, D. clarus, D. myceliophagus, and D. nanus. DNA sequencing data was obtained on the partial 18S, D2/D3 expansion segments of the 28S rRNA gene and internal transcribed spacer (ITS). The phylogenetic relationships of this species with other Ditylenchus spp. using partial 18S–rDNA and D2/D3 indicated that D. stenurus n. sp. clustered together with several species belongs to the D. triformis-group i. e. D. africanus, D. destructor and D. halictus: all sharing a rounded tail terminus and six lines in lateral fields. 相似文献