Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells     

Giuliano Pisoni  Patrizia D’Amelio  Francesca Sassi  Giovanni Manarolla  Licia Scaccabarozzi  Clara Locatelli  Nausicaa Mazzocchi  Mirko Baruscotti  Dario DiFrancesco  Paolo Moroni 《Veterinary journal (London, England : 1997)》2011,189(3):361-363

Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.  相似文献   

Heavy Metal Concentration Survey in Soils and Plants of the Les Malines Mining District (Southern France): Implications for Soil Restoration     

Jose Escarr��  Claude Lef��bvre  Stephan Raboyeau  Anabelle Dossantos  Wolf Gruber  Jean Claude Cleyet Marel  Helene Fr��rot  Nausicaa Noret  Stephanie Mahieu  Christian Collin  Folkert van Oort 《Water, air, and soil pollution》2011,216(1-4):485-504

Mining activities generate spoils and effluents with extremely high metal concentrations of heavy metals that might have adverse effects on ecosystems and human health. Therefore, information on soil and plant metal concentrations is needed to assess the severity of the pollution and develop a strategy for soil reclamation such as phytoremediation. Here, we studied soils and vegetation in three heavily contaminated sites with potential toxic metals and metalloids (Zn, Pb, Cd, As, TI) in the mining district of Les Malines in the Languedoc region (southern France). Extremely high concentrations were found at different places such as the Les Aviniéres tailing basins (up to 160,000 mg kg^?C1 Zn, 90,000 mg kg^?C1 Pb, 9,700 mg kg^?C1 of As and 245 mg kg^?C1 of Tl) near a former furnace. Metal contamination extended several kilometres away from the mine sites probably because of the transport of toxic mining residues by wind and water. Spontaneous vegetation growing on the three mine sites was highly diversified and included 116 plant species. The vegetation cover consisted of species also found in non-contaminated soils, some of which have been shown to be metal-tolerant ecotypes (Festuca arvernensis, Koeleria vallesiana and Armeria arenaria) and several Zn, Cd and Tl hyperaccumulators such as Anthyllis vulneraria, Thlaspi caerulescens, Iberis intermedia and Silene latifolia. This latter species was highlighted as a new thallium hyperaccumulator, accumulating nearly 1,500 mg kg^?C1. These species represent a patrimonial interest for their potential use for the phytoremediation of toxic metal-polluted areas.  相似文献