Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers          下载免费PDF全文

Kapil K. Tiwari  Anshuman Singh  Sasmita Pattnaik  Maninder Sandhu  Sukhdeep Kaur  Sourabh Jain  Sushma Tiwari  Shweta Mehrotra  Mahender Anumalla  Rashmita Samal  Jyoti Bhardwaj  Neha Dubey  Vikrant Sahu  Gayle A. Kharshing  Patu K. Zeliang  Kadiri Sreenivasan  Pankaj Kumar  Swarup K. Parida  Sevanthi V. A. Mithra  Vandana Rai  Wricha Tyagi  Pawan K. Agrawal  Atmakuri R. Rao  Arunava Pattanayak  Girish Chandel  Ashok K. Singh  Ishwari S. Bisht  Kangila V. Bhat  Gundimeda J. N. Rao  Jitendra P. Khurana  Nagendra K. Singh  Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171

Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.  相似文献   

Hyperkalemic periodic paralysis and the adult muscle sodium channel alpha-subunit gene   总被引：22，自引：0，他引：22  

B Fontaine  T S Khurana  E P Hoffman  G A Bruns  J L Haines  J A Trofatter  M P Hanson  J Rich  H McFarlane  D M Yasek 《Science (New York, N.Y.)》1990,250(4983):1000-1002

Hyperkalemic periodic paralysis (HYPP) is an autosomal dominant disorder characterized by episodes of muscle weakness due to depolarization of the muscle cell membrane associated with elevated serum potassium. Electrophysiological studies have implicated the adult muscle sodium channel. Here, portions of the adult muscle sodium channel alpha-subunit gene were cloned and mapped near the human growth hormone locus (GH1) on chromosome 17. In a large pedigree displaying HYPP with myotonia, these two loci showed tight linkage to the genetic defect with no recombinants detected. Thus, it is likely that the sodium channel alpha-subunit gene contains the HYPP mutation.  相似文献   

Studies on blood coagulation of goats in India. II. Prothrombin time and prothrombin activity     

S C Adaval  J S Khurana  D P Mukerji 《The Indian veterinary journal》1966,43(2):101-105

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Salmonella serotypes in selected classes of food animal carcasses and raw ground products, January 1998 through December 2000     

Rigney CP  Salamone BP  Anandaraman N  Rose BE  Umholtz RL  Ferris KE  Parham DR  James W 《Journal of the American Veterinary Medical Association》2004,224(4):524-530

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Identification of a dynamic atmosphere at Enceladus with the Cassini magnetometer     

Dougherty MK  Khurana KK  Neubauer FM  Russell CT  Saur J  Leisner JS  Burton ME 《Science (New York, N.Y.)》2006,311(5766):1406-1409

The Cassini magnetometer has detected the interaction of the magnetospheric plasma of Saturn with an atmospheric plume at the icy moon Enceladus. This unanticipated finding, made on a distant flyby, was subsequently confirmed during two follow-on flybys, one very close to Enceladus. The magnetometer data are consistent with local outgassing activity via a plume from the surface of the moon near its south pole, as confirmed by other Cassini instruments.  相似文献   

A systematic survey of loss-of-function variants in human protein-coding genes     

MacArthur DG  Balasubramanian S  Frankish A  Huang N  Morris J  Walter K  Jostins L  Habegger L  Pickrell JK  Montgomery SB  Albers CA  Zhang ZD  Conrad DF  Lunter G  Zheng H  Ayub Q  DePristo MA  Banks E  Hu M  Handsaker RE  Rosenfeld JA  Fromer M  Jin M  Mu XJ  Khurana E  Ye K  Kay M  Saunders GI  Suner MM  Hunt T  Barnes IH  Amid C  Carvalho-Silva DR  Bignell AH  Snow C  Yngvadottir B  Bumpstead S  Cooper DN  Xue Y  Romero IG; Genomes Project Consortium  Wang J  Li Y  Gibbs RA  McCarroll SA  Dermitzakis ET  Pritchard JK  Barrett JC 《Science (New York, N.Y.)》2012,335(6070):823-828

Genome-sequencing studies indicate that all humans carry many genetic variants predicted to cause loss of function (LoF) of protein-coding genes, suggesting unexpected redundancy in the human genome. Here we apply stringent filters to 2951 putative LoF variants obtained from 185 human genomes to determine their true prevalence and properties. We estimate that human genomes typically contain ~100 genuine LoF variants with ~20 genes completely inactivated. We identify rare and likely deleterious LoF alleles, including 26 known and 21 predicted severe disease-causing variants, as well as common LoF variants in nonessential genes. We describe functional and evolutionary differences between LoF-tolerant and recessive disease genes and a method for using these differences to prioritize candidate genes found in clinical sequencing studies.  相似文献   

Effect of dispersing agents on synthesis of nano titanium oxide and its application for antimicrobial property     

N. Khurana  R. V. Adivarekar 《Fibers and Polymers》2013,14(7):1094-1100

With the growing demand for comfortable, clean and hygienic textile goods, an urgent need for production of antimicrobial textiles has arisen. In this work, synthesis of nano titanium oxide has been done by sol gel method and the effect of dispersing agents with varying ionic character during synthesis on the particle size of the synthesised nano particles in terms of crystallinity and particle size was studied. It was observed that cationic as well as non-ionic dispersing agents led to reduction in size of the titania particles produced whereas anionic dispersing agent led to increase in the particle size. This method provides a simple method for reduction in size of titanium dioxide particles with a minor modification of a standard process. The synthesized nano titanium dioxide particles were successfully applied onto cotton to obtain Antimicrobial activity. The Durability of the antimicrobial activity was upto 10 washes with the help of Binder. The effect of the application of nano titanium oxide on the physical properties of the substrate, such as tensile strength, bending length, and crease recovery angle has been analysed.  相似文献   

Socio-Economic and Scientific Impact Created by Whitefly-Transmitted,Plant-Virus Disease Resistant Tomato Varieties in Southern India          下载免费PDF全文

John Colvin  N Nagaraju  Carlos Moreno-Leguizamon  R M Govindappa  T B Manjunatha Reddy  SA Padmaja  Neena Joshi  Peter M Hanson  Susan E Seal  V Muniyappa 《农业科学学报》2012,11(2):337-345

Research carried out to assess the impact of open-pollinated Tomato leaf curl virus (ToLCV)-resistant tomatoes and hybrids on the livelihoods of resource-poor farmers in Southern India is described and discussed. Three high-yielding ToLCV-resistant tomato varieties were developed initially using conventional breeding and screening techniques involving inoculation by ToLCV-viruliferous whitefly, Bemisia tabaci. In 2003 and 2004, respectively, these varieties were released officially by the Karnataka State Seed Committee and the Indian Ministry of Agriculture through notification in the Gazette of India. From 2003 to 2005, eleven seed companies bought breeder seed of the ToLCV-resistant varieties and used them to begin breeding F₁ hybrids from them. Socio-economic studies carried out to assess the benefits obtained from growing the ToLCV-resistant varieties found that farmers could gain up to 10 times the profit by growing the ToLCV-resistant varieties compared to the pre-existing ToLCV-susceptible varieties. Adoption of ToLCV-resistant tomatoes was also associated with reduced pesticide use. Extra income from tomato sales was prioritised by farmers to pay for children's education, better nutrition and medicines. In a joint effort with the commercial seed sector in India, a promotional field day was organised in 2007. As well as the three ToLCV-resistant varieties, 62 ToLCV-resistant hybrid tomatoes were exhibited during a farmer-field day by 17 commercial seed companies and several public institutes. Tomatoes with ToLCV-resistance are now grown widely in South India and seeds of the three open-pollinated varieties have been distributed to more than 12 countries. In 2007, a conservative estimate of the financial-benefit to cost of the research ratio was already more than 837:1.  相似文献   

Removal of Dyes Using Graphene-Based Composites: a Review     

Ishani Khurana  Amit Saxena  Bharti  Jitender M. Khurana  Pramod Kumar Rai 《Water, air, and soil pollution》2017,228(5):180

Water contamination has reached an alarming state due to industrialization and urbanization and has become a worldwide issue. Dyes contaminate water and are addressed extensively by researchers. Various technologies and materials have been developed for the treatment of contaminated water. Among them, adsorption has attracted great attention due to its ease and cost-effective nature. In recent years, graphene-based composites have shown great potential for the removal of contaminants from water. The literature reveals the usefulness of composites of graphene with metal oxides, carbon derivatives, metal hybrids and polymers for the removal of organic dyes from contaminated water. In this review, efforts have been made to compile the studies on the removal of cationic and anionic dyes from water using graphene-based composites.  相似文献   

Physiological response of cauliflower in relation to manganese-boron interaction     

C. Chatterjee  P. Sinha  N. Nautiyal  N. Khurana  S. C. Agarwala 《The Journal of Horticultural Science and Biotechnology》2013,88(5):591-596

Summary

Cauliflower (Brassica oleracea L. var. botrytis) was grown in refined sand at low (0.0011 mg l^?1), normal (0.55 mg l^?1) and excess (5.5 mg l^?1) Mn, each at three levels of B, deficient (0.0033 mg l^?1), normal (0.33 mg H) and excess (3.3 mg l^?1). In Mn deficient cauliflowers a deficiency of B accentuated visible symptoms of Mn deficiency and aggravated the Mn deficiency effects i.e. a decrease in dry matter, leaf Mn, sugars, starch, chlorophyll, Hill reaction and specific activity of aldolase and an increase in the concentrations of proline and inorganic phosphorus. In cauliflowers exhibiting B toxicity symptoms under conditions of excess B, excess Mn increased leaf Mn, sugars, nucleic acids, protein P, nucleic acid P, acid-labile P and leaf B. Excess Mn decreased the concentrations of DNA, protein nitrogen, chlorophyll and activities of peroxidase, aldolase and leaf B, leaf Mn in boron deficient cauliflowers.  相似文献