Biocontrol of soil diseases and soil profile associated with rhizosphere of rice (Oryza sativa Subsp. Japonica) growing paddy fields in Kansai region,Japan     

Hosne Ara Dilzahan  Masaaki Yoshida  Maya Umeda  Michelle Ann Calubaquib  Nolissa Delmo Organo  Andre Freire Cruz 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(5):444-455

ABSTRACT

This research focused on in vitro and in vivo biocontrol of two major rice diseases, Bacterial Leaf Blight caused by Xanthomonus oryzae pv oryzae (Xoo) and Sheath Blight caused by Rhizoctonia solani. Furthermore, the soil of paddy fields in Kansai region, Japan was analysed for the presence of these pathogens, and their microbial communities were evaluated according to the location and management systems. For biocontrol, the rhizospheric bacteria were isolated and molecularly identified. The results indicated that DAR17225040 and DAR17225017 were 99% similar to Bacillus aryabhattai and Bacillus megaterium. The isolated bacteria were able to suppress Bacterial Leaf Blight and Sheath Blight disease 78% and 86% respectively, under greenhouse conditions. The 16S amplicon sequencing (Illumina MiSeq 250bp) was used to analyse the bacterial community in paddy fields of soil taken from five locations under conventional and organic systems. Xoo and R. solani were detected in sampled soil, however, no clear difference was noted, regarding systems. From amplicon sequencing data, Chloroflexi, Acidobacteria, Proteobacteria, Actinobacteria, Planctomycetes were the dominant phyla of rice soil in Kansai region. The phylum Kazan-3B-28 was found more in organic system than conventional. The bacterial community was more affected by location and soil type than by management systems.  相似文献   

Effects of nanofertilizer and nano-plant hormone on soil chemical properties and microbial community in two different soil types     

John Lester Viscara PIDE  Nolissa Delmo ORGANO  Andre Freire CRUZ  Lilia M. FERNANDO  Lucille C. VILLEGAS  Evelyn F. DELFIN  Michelle Ann Magat CALUBAQUIB  Roselle Estera MADAYAG  Erlinda S. PATERNO 《土壤圈》2023,33(5):765-775

Application of nanotechnology in agriculture has been expanded to improve crop production. The impact of nanomaterials (NMs) on factors that influence the survival and function of beneficial microorganisms is a less studied aspect that needs to be better understood. Only a few studies have assessed the effects of NMs on beneficial soil microorganisms. This study was conducted to assess the effects of nanofertilizer FertiGroe^?N (FG-N) and nano-plant hormone HormoGroe^?auxin (...  相似文献