Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis     

Thomas H Hansen  Kristian H Laursen  Daniel P Persson  Pai Pedas  Søren Husted  Jan K Schjoerring 《Plant methods》2009,5(1):12-11

Background  

Quantitative multi-elemental analysis by inductively coupled plasma (ICP) spectrometry depends on a complete digestion of solid samples. However, fast and thorough sample digestion is a challenging analytical task which constitutes a bottleneck in modern multi-elemental analysis. Additional obstacles may be that sample quantities are limited and elemental concentrations low. In such cases, digestion in small volumes with minimum dilution and contamination is required in order to obtain high accuracy data.  相似文献   

Eco‐efficiency as a strategy for optimizing the sustainability of pest management     

Roger D Magarey  Sarah SH Klammer  Thomas M Chappell  Christina M Trexler  Godshen R Pallipparambil  Ernie F Hain 《Pest management science》2019,75(12):3129-3134

Agricultural industrialization and the subsequent reliance on pesticides has resulted in numerous unintended consequences, such as impacts upon the environment and by extension human health. Eco‐efficiency is a strategy for sustainably increasing production, while simultaneously decreasing these externalities on ecological systems. Eco‐efficiency is defined as the ratio of production to environmental impacts. It has been widely adopted to improve chemical production, but we investigate the challenges of applying eco‐efficiency to pesticide use. Eco‐efficiency strategies include technological innovation, investment in research and development, improvement of business processes, and accounting for market forces. These components are often part of integrated pest management (IPM) systems that include alternatives to pesticides, but its implementation is often thwarted by commercial realities and technical challenges. We propose the creation and adoption of an eco‐efficiency index for pesticide use so that the broad benefits of eco‐efficient strategies such as IPM can be more readily quantified. We propose an index based upon the ratio of crop yield to a risk quotient (RQ) calculated from pesticide toxicity. Eco‐efficiency is an operational basis for optimizing pest management for sustainability. It naturally favors adoption of IPM and should be considered by regulators, researchers, and practitioners involved in pest management. © 2019 Society of Chemical Industry  相似文献   

Plants as de-worming agents of livestock in the Nordic countries: historical perspective, popular beliefs and prospects for the future.     

PJ Waller  G Bernes  SM Thamsborg  A Sukura  SH Richter  K Ingebrigtsen  J H?glund 《Acta veterinaria Scandinavica》2001,42(1):31-44

Preparations derived from plants were the original therapeutic interventions used by man to control diseases (including parasites), both within humans and livestock. Development of herbal products depended upon local botanical flora with the result that different remedies tended to develop in different parts of the world. Nevertheless, in some instances, the same or related plants were used over wide geographic regions, which also was the result of communication and/or the importation of plant material of high repute. Thus, the Nordic countries have an ancient, rich and diverse history of plant derived anthelmintic medications for human and animal use. Although some of the more commonly used herbal de-wormers were derived from imported plants, or their products, many are from endemic plants or those that thrive in the Scandinavian environment. With the advent of the modern chemotherapeutic era, and the discovery, development and marketing of a seemingly unlimited variety of highly efficacious, safe synthetic chemicals with very wide spectra of activities, herbal remedies virtually disappeared from the consciousness--at least in the Western world. This attitude is now rapidly changing. There is a widespread resurgence in natural product medication, driven by major threats posed by multi-resistant pest, or disease, organisms and the diminishing public perceptions that synthetic chemicals are the panacea to health and disease control. This review attempts to provide a comprehensive account of the depth of historical Nordic information available on herbal de-wormers, with emphasis on livestock and to provide some insights on potentially rewarding areas of "re-discovery" and scientific evaluation in this field.  相似文献   

Characterization of monoclonal antibodies against avian reovirus S1133 protein sigmaA synthesized in Escherichia coli     

Pai WC  Shien JH  Liu HJ  Lee LH 《Veterinary microbiology》2003,91(4):309-323

Monoclonal antibodies (MAbs) were prepared against avian reovirus S1133 protein sigmaA (esigmaA) synthesized in Escherichia coli. MAbs were characterized and used to develop a diagnostic test. Ten MAbs were selected for competitive binding assay following coupling with horseradish peroxidase. The results indicated that these MAbs delineated two epitopes I and II of esigmaA. An immuno-dot binding assay was used to detect the effect of denaturation on antibody recognition of the epitopes. All MAbs bound to esigmaA in its native form. After denaturation by boiling in SDS and 2-mercaptoethanol, the binding of MAbs recognizing epitope I was fully abolished. However, the reactivity of MAbs recognizing epitope II was not affected. MAbs 31 and 32, recognizing epitopes I and II, respectively, were selected for the cross-reactivity to heterologous reovirus strains. The results suggest that the two epitopes are highly conserved among these virus strains. A MAb capture enzyme-linked immunosorbent assay (ELISA) procedure was developed using MAbs 32 and 31 to detect reovirus protein sigmaA in samples from tendon tissues of infected bird and chicken embryo fibroblast (CEF) cell cultures. Avian reovirus sigmaA antigens in tendon specimens were detected from the inoculated birds as early as 2 days post-inoculation (PI), approximated a peak at 7 days PI, and maintained this until 16 days PI, then decreased gradually. A clear difference in absorbance values between the tendon samples of the avian reovirus- and mock-infected birds is obtained. Positive results were also obtained from avian reovirus-infected CEF and from the tendon tissues of naturally infected broilers. These results indicated that the MAb capture ELISA is a useful methods for the detection of avian reovirus from chickens suspected to have avian reovirus infections.  相似文献   

大兴安岭林区森林抚育工程系统输出特性和转化特性的研究     

杨雪峰  樊冬温  李应珍  李湃 《森林工程》2015,(1):34-37

为提高大兴安岭林区森林抚育工程作业质量和工程成效,采用系统工程的分析方法,根据大兴安岭林区生态环境、功能和林木的结构特点,对大兴安岭林区最主要的三种抚育类型(透光抚育、生长伐抚育、生态疏伐抚育)的直接输出特性、长效输出特性和转化特性进行了详细分析。在此基础上,利用运行控制和统计学理论,通过调整输出特性偏移量、输出特性标准偏差和输出特性公差要求,提高工程能力,进而优化输出特性,最终实现提高工程作业质量和工程成效的目的。对输出特性和转化特性的分析研究,为大兴安岭林区森林抚育工程技术要求标准的产出提供基础和支撑。  相似文献   

Ultrasonographic biometry of the normal eye of the Persian cat     

A Mirshahi  SH Shafigh  M Azizzadeh 《Australian veterinary journal》2014,92(7):246-249

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烟草生产全程机械化作业研究     

余铭华  林绍武  施恩  郝东  徐恒光  李静超  李名荣 《湖南农业科学》2014,(20)

为了探索烟叶生产过程中各机械的性能及工作效率,2013年在福建省建阳市莒口镇河坝新村开展了烟叶生产全程机械化作业试点研究。结果表明,起垄环节,碎土率方面邵武华友履带式起垄机表现最好(89.9%),沟底积土方面松溪永顺表现最好;覆膜环节,各测试机型表现中等,在效率方面优于人工覆膜;移栽环节,漏栽率上江苏久泰移栽机高于江苏井关移栽机;培土环节,漳州南冠培土机操作简便,掉头灵活,单人操作,单行双侧作业,深受烟农喜爱;拔杆环节,龙岩诚德拔杆机单人操作,操作简便,动力较足,拔杆顺畅,拔杆带土较少,效果较好,适应性较强。  相似文献   

中国良种推广补贴政策实践及启示     

蔡派 《世界农业》2007,(6):26-28

中国加入WTO以来,中国良种补贴政策开始逐步实施。补贴的品种、范围和规模不断扩大,其成效也越来越明显。笔者认为:良种补贴已成为中国农业补贴的重要内容,是一项重要的支农惠民政策。良种补贴符合农业科技发展的实际,有效地推动了科技贡献率的提高,已成为一项提升粮食综合生产能力的重大发展政策。  相似文献   

Fiber Types Distribution in the Digastric Muscle of Tufted Capuchin Monkey (Cebus apella)     

J. C. Andreo  V. D. Pai    J. A. C. Navarro  J. A. DE  Oliveira 《Anatomia, histologia, embryologia》1994,23(3):226-231

Fiber types distribution in the diagastric muscle of tufted capuchin monkey was studied by means of NADH-TR, myosin-ATPase, after alkaline and acid preincubations and SDH histochemical reactions. Three different types of fibers were found presenting an equal distribution. The percentage and types of fibers were as follow: 18.2 % SO (Slow Oxydative), 38.4 % FOG (Fast Oxydative Glycolytic) and 43.4 % FG (Fast Glycolytic). FG fibers revealed the largest area. The relatively high concentration of fast twitch (81.2 %) seems to indicate this muscle is involved with the acceleration and fast speed of jaw movements. Aerobic metabolism represented by SO + FOG fibers (56.6 %) suggestes that this muscle possesses an additional role than that related to the lowering of the jaw.  相似文献   

Microsporum gypseum dermatophytosis in a rabbit   总被引：1，自引：0，他引：1  

S Weisbroth SH SCHER 《Journal of the American Veterinary Medical Association》1971,159(5):629-634

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