Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer     

Nafiseh Raji  Tayebeh Majidi Zadeh  Pegah Babheidarian  Massoud Houshmand 《Iranian Biomedical Journal》2022,26(1):70

Background: Colorectal cancer is the third most common cancer worldwide. MSI is a molecular marker of a deficient MMR system and happens in almost 15% of CRCs. Because of a wide frequency of MSI⁺ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detection technique is a fluorescent PCR-based method in which fragments are analyzed by CE. This technique is very time-consuming, difficult, and expensive. We sought to develop and evaluate a proper method with high accuracy, specificity, and sensitivity to screen the MSI⁺ CRC. A HRM analysis procedure is relying on the analysis of the melting curve attributes. Low cost, feasibility, high specificity, and sensitivity are outstanding attributes of HRM analysis. Results: Five mononucleotide microsatellite markers, including BAT-25, BAT-26, NR-21, NR-24, and NR-27, in 25 archival CRC tumor tissue samples were compared with normal tissue adjacent using HRM method. The specificity and sensitivity of BAT-25 with HRM method were 100% compared to CE, while other markers had lower sensitivity. However, when all the markers were considered together, the sensitivity and specificity became 100%. The number of MSI⁺ samples was 56%, which shows a higher ratio than previous Iranian studies. The highest MSI was related to BAT-26 (52%). Conclusion: The HRM method is much simpler and more cost-effective than current MSI techniques, and its sensitivity and accuracy are comparable. Therefore, it can serve as an alternative method in cases where CE is unavailable. Key Words: Biomarkers, Microsatellite instability, Colorectal cancer  相似文献   

Role of mitotic inhibitors and genotype on chromosome doubling of <Emphasis Type="Italic">Rosa</Emphasis>     

Pegah Khosravi  Maryam Jafarkhani Kermani  Ghorban Ali Nematzadeh  Mohammad Reza Bihamta  Kazutomo Yokoya 《Euphytica》2008,160(2):267-275

Dinitroanilines represent a class of compounds that are widely used in herbicide formulations as they depolymerise plant microtubles, causing chromosome doubling. The potential of microtubule depolymerising herbicides trifluralin, oryzalin, and amiprophosmethyl (APM) for in vitro chromosome doubling of Rosa was studied. Five concentrations (0, 3, 6, 12 and 24 μM) and three exposure periods (12, 24 and 48 h) for each of the compounds were compared. Oryzalin, trifluralin and APM were not significantly different in their ability to induce chromosome doubling of R. hybrida cv Iceberg. At concentration of 6 μM and exposure period of 24 h, chromosome doubling of R. hybrida cv Iceberg was not significantly different with each of the polyplodising agents. At higher concentration (24 μM) and longer exposure period (48 h), 66.7% and 62.5% chromosome doubling was achieved with APM and trifluralin, respectively. However, the application of 6 μM oryzalin to R. persica (2n = 2x), R. hybrida cv Iceberg (2n = 3x) and R. hybrida cv Akito (2n = 4x), resulted in 60.0%, 6.3% and 0% chromosome doubling, respectively, which suggest that chromosome doubling is genotype dependent and plants with lower ploidy level have a higher propensity for chromosome doubling. Flow cytometry results at 18 and 24 weeks after herbicide treatment, indicated that the best time to test the treated plants was after 24 weeks.  相似文献   

Sugar beet yield response to different levels of saline irrigation water and leaching in an arid region     

Mohammad Feizi  Pegah Noorshargh 《Journal of plant nutrition》2018,41(5):654-663

The main objective of this work was to evaluate the effects of saline irrigation water and leaching on the sugar beet yield components. In a field experiment in Rudasht region (Isfahan, Iran), three irrigation water salinity levels (1.6, 8.1, and 12.3 dS m^?1) and with/without leaching were applied. The experimental units comprised of a completely randomized block design, with split plot in four replications. The results indicated that the white sugar yield and alkalinity decreased by increasing the water salinity. Salts leaching significantly increased the root yield, white sugar yield, and white sugar concentration. With higher levels of water salinity molasses sugar, leaf weight, and the concentrations of Na, K, and α- amino-N in sugar beet significantly increased. Consequently, it appears that the use of drainage water in combination with fresh water could be recommended as a strategic management way to grow sugar beet in the investigated arid region.  相似文献   

Response of maize (Zea mays L.) to potassium nano-silica application under drought stress     

Pegah Aqaei  Marjan Diyanat  Javad Razmi  Paul C. Struik 《Journal of plant nutrition》2020,43(9):1205-1216

Abstract

To investigate the influence of potassium nano-silica (PNS) on maize plant under drought stress including non-stress (NS), moderate drought stress (MDS) and severe drought stress (SDS), a factorial experiment was conducted with completely randomized blocks with three replications. Drought stress decreased the concentrations in the shoot of phosphor (P), calcium (Ca), iron (Fe), zinc (Zn), manganese (Mn) and silica (Si) and nitrogen (N), P, Ca, Fe, Zn, copper (Cu), Mn and Si concentrations of seed. There was an increase in the concentration in the N seed and shoot potassium (K) concentration under drought stress. It was observed that applying PNS increased nutrient absorption. The highest concentration of N in the seed was obtained at 100?ppm PNS. The highest concentrations of seed K and N, Cu, Mn and Si in the shoot were found when 200?ppm of PNS was applied. Applying PNS had no significant effect on the concentrations of P, Ca, sodium (Na) and Cu in the seed, and of Ca and Na in the shoot. These findings demonstrate that the application of PNS can limit the negative effects of drought stress and improve plant’s resistance against drought stress.  相似文献   

Pyogenic bacterial infections in humans with MyD88 deficiency     

von Bernuth H  Picard C  Jin Z  Pankla R  Xiao H  Ku CL  Chrabieh M  Mustapha IB  Ghandil P  Camcioglu Y  Vasconcelos J  Sirvent N  Guedes M  Vitor AB  Herrero-Mata MJ  Aróstegui JI  Rodrigo C  Alsina L  Ruiz-Ortiz E  Juan M  Fortuny C  Yagüe J  Antón J  Pascal M  Chang HH  Janniere L  Rose Y  Garty BZ  Chapel H  Issekutz A  Maródi L  Rodriguez-Gallego C  Banchereau J  Abel L  Li X  Chaussabel D  Puel A  Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696

MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections.  相似文献   

Utilization of Whole Exome Sequencing in Non-Syndromic Premature Ovarian Failure: Ficolin-3 Gene Mutation in an Iranian Family     

Soophia Mehrjooy  Roshan Nikbakht  Javad Mohammadi Asl  Ataallah Ghadiri  Pegah Ghandil 《Iranian Biomedical Journal》2021,25(6):441

Background:Premature ovarian failure is a heterogeneous disorder, leading to early menopause. Several genes have been identified as the cause of non-syndromic POF. Our aim was to explore the genetic defects in Iranian patients with POF. Methods:We studied a family with three females exhibiting non-syndromic POF. WES was performed for one of the affected individuals after ruling out the presence of CGG repeat expansion at FMR1 gene in the family. Sanger sequencing was used to confirm the candidate sequence variants in the proband, and screening of the detected mutation was performed for the other affected and unaffected members of the family. Results:A homozygous frameshift mutation, c.349delC, was identified in FCN3 gene in the proband and two other patients. The parents and two healthy brothers were heterozygous for the mutation, and an unaffected sister was homozygous for wild type. Conclusion:This is the first report of a mutation in FCN3 gene in a family with POF. Our findings can lead to the enhancement of genetic databases of patients with POF, specifically for families with high-risk background. Key Words: Ficolin-3, Premature ovarian failure, Whole exome sequencing  相似文献