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An ancient developmental induction: heat-shock proteins induced in sporulation and oogenesis 总被引：54，自引：0，他引：54
Every eukaryotic and prokaryotic organism tested to date synthesizes a small number of heat-shock proteins in response to heat and other forms of stress. A particular pattern of heat-shock gene expression was observed during ascospore development in Saccharomyces: heat-shock proteins hsp26 and hsp84 were strongly induced nor inducible by heat shock. Instead, two proteins related to hsp70 were induced. A strikingly similar pattern of expression occurs during oogenesis in Drosophila, suggesting that it may be one of the earliest developmental pathways to evolve in eukaryotic cells. 相似文献
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P Georgiev H Bostedt S Goericke‐Pesch M Dimitrov P Petkov K Stojanthev V Tsoneva A Wehrend 《Reproduction in domestic animals》2010,45(5):e161-e167
The aim of this study was to test for the efficacy and safety of the use of aglepristone for pregnancy termination on day 45 in cats. Six healthy cats were treated with 10 mg/kg aglepristone sc on day 45 and 46 after mating; six other cats served as untreated controls. The effect of treatment was monitored by general examination, vaginal cytology, ultrasonography and blood sampling for haematology and progesterone determination. Besides, interoestrus interval and next pregnancy including litter size were recorded. The efficacy of treatment was approximately 67% (4/6) with abortion occurring 4–7 days after the first injection and a sanguineous discharge and erythrocytes in vaginal smears for at least 6 days afterwards. The two treated cats that did not abort gave birth to two kittens on day 67 and had a stillbirth of a single kitten on day 71, respectively. As expected enlargement of the mammary glands and lactation were observed in all treated cats. No other treatment‐induced side effects were observed. Progesterone levels at abortion were high (30–140 nmol/l), but were decreased on day 55. Aglepristone treatment did not affect fertility in following cycles. Finally, it can be concluded that late‐term pregnancy termination with aglepristone is possible but due to a success rate of 67% an ultrasonographical examination 7 days after treatment is an inherent necessity to control the effect of treatment. 相似文献
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M. Mikulič Petkovšek F. Štampar R. Veberič 《Physiological and Molecular Plant Pathology》2009,74(1):60-67
The research dealt with phenolics in healthy versus scab infected apple leaves and fruits. The leaf samples were picked in the period from May to September and the fruit samples at technological maturity. Infection with the Venturia inaequalis fungus enhanced the metabolism of phenolics at the infected sites, especially in the boundary tissue. Tissue infected with Venturia inaequalis showed in comparison to the healthy tissue up to 7.6 times more hydroxycinnamic acids, up to 2.6 times more flavan-3-ols and up to 2.9 times higher values of flavanols. The content level of total phenolics in the infected tissue was 1.3–2.4 times higher than in the healthy leaves and fruit. 相似文献
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Gregor Petkovšek 《Journal of Soils and Sediments》2018,18(10):3153-3165
Purpose
This paper presents the development of a turbid density current module (TDCM) for a one-dimensional quasi-steady reservoir sedimentation model suitable for long-term simulations and an example of its application.Materials and methods
The module determines the plunging point of a density current based on the criterion of the densimetric Froude number. Following plunging, simulation continues in a supercritical regime as long as the energy of density flow and bed slope are sufficient to support transport in this mode, or until the dam is reached. A muddy pond is then formed. An adapted version of the theory of Toniolo et al. (2007) is used to compute water and sediment balance in a muddy pond. If the level of muddy pond exceeds the lowest level outlet, sediment is vented from the reservoir.Results and discussion
The model was applied to one of the largest reservoirs in the world, the Nurek reservoir in Tajikistan. Historical data on bed levels and sediment inflow as well as a recent survey from 2015 were used to calibrate and validate the model. The model showed good results both in terms of modelled bed levels and composition of deposited sediment. For comparison purpose, the model was also calibrated without the TDCM module. However, the discrepancy between the observed and modelled data was significantly higher, confirming that the good match in the case of the TDCM module was not achieved solely by calibration, but rather by a more appropriate approach to modelling.Conclusions
These results support the choice of using the model with TDCM for modelling a general pattern of long-term deposition in the Nurek reservoir, in which turbid density currents play an important role.8.
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Aleksandar Dimitrov Svetlana Genieva Petko Petkov Lyubomir Vlaev 《Water, air, and soil pollution》2012,223(8):5087-5095
Black rice husk ash (BRHA) was obtained by means of thermal degradation of raw rice husks (RRH) on a pilot plant fluidized bed reactor. BRHA was characterized using chemical analyses, scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and thermal analysis. The kinetics was studied using batch adsorption technique and on the basis of prior characterization by X-ray diffraction patterns and scanning electron microscopy. The adsorption capacities of diesel fuel at 288, 293 and 298?K onto BRHA were determined. Results showed that the material studied has very high adsorption capacity and low cost and may successfully be used as an effective adsorbent to clean up spills of oil products in water basins. The adsorption of diesel fuel onto BRHA proceeds rapidly to reach adsorption equilibrium in about 10?min. The saturated BRHA can be burnt in incinerators, industrial ovens or steam generators, and through this way ecological and economic benefits are attained. 相似文献
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O Oestrup V Hall SG Petkov XA Wolf S Hyldig P Hyttel 《Reproduction in domestic animals》2009,44(S3):39-49
The increasing focus on the pig as a biomedical model calls for studies which investigate morphological and molecular mechanisms during initial embryonic development in this species. In the pig, the paternal genome is actively demethylated in the zygote, whereas the maternal genome remains methylated. The major genome activation occurs at the four-cell stage, when prominent ribosome-synthesizing nucleoli develop in the blastomeres, allowing for trophectoderm and inner cell mass (ICM) differentiation. Unlike in mice, the pluripotency gene OCT4 is initially expressed in both compartments. The ICM differentiates into epiblast and hypoblast approximately at the time of hatching from the zona pellucida, and subsequently the loss of the Rauber's layer results in an uncovered epiblast establishing the embryonic disc again in contrast to mice. This particular and protracted ICM/epiblast biology may contribute to the lack of success in culturing porcine embryonic stem cells. The embryonic disc subsequently becomes polarized by a posterior thickening, which includes ingression of the first extra-embryonic mesoderm. Thereafter, the primitive streak forms and gastrulation results in formation of the somatic germ layers and germline, i.e. the primordial germ cells. The latter remain pluripotent for a period and may be isolated and cultured as embryonic germ cells in vitro . 相似文献