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Daniela Bertotto Carlo Poltronieri Elena Negrato Duilio Majolini Giuseppe Radaelli Claudia Simontacchi 《Aquaculture Research》2010,41(8):1261-1267
Plasma cortisol is the most commonly used indicator of stress in fish but, as the blood sampling procedure itself can be a source of stress, it would be helpful to measure cortisol using less invasive matrices. It is also necessary to find alternative matrices as stress indicators in dead fish in which blood sampling is impossible. In the present study, we investigated transport stress in three aquaculture species, European sea bass (Dicentrarchus labrax L.), common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum), by cortisol determination (radioimmunoassay) in plasma and other matrices (skin mucus, gut content, lateral muscle and caudal fin). Cortisol significantly increased after transport in all species and matrices, except in the sea bass gut content, where it remained unchanged. The three species responded to transport stress by producing different cortisol levels. In conclusion, the significant correlation found between plasma cortisol and most of the other matrices opens up the possibility of using them to evaluate stress in fish: mucus sampling is a less invasive method than blood sampling, and in addition to muscle and fin sampling, it can be used in postmortem fish. 相似文献
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Carlo Poltronieri Rosaria Laurà Daniela Bertotto Elena Negrato Claudia Simontacchi Maria Cristina Guerrera Giuseppe Radaelli 《Veterinary research communications》2009,33(7):619-629
Farmed fish are usually exposed to routine procedures which have strong effects on stress responses. Rodlet cells may represent
an useful biomarker for studies on the presence of stressors in aquaculture. This work focused on the localization of rodlet
cells by light and electron microscopy in gills of sea bass (Dicentrarchus labrax) subjected to different conditions of overcrowding. In general, a significant increase in number of rodlet cells has been
observed in all animals subjected to overcrowding stress. In gills of control group rare rodlet cells were detected at the
level of both primary and secondary lamellae, whereas in stressed group clusters of rodlet cells have been found in the epithelium
of primary and secondary lamellae indicating that these cells are influenced by stocking density. 相似文献
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Vettorazzi Julio Cesar Fiorio Santa Catarina Renato Poltronieri Tathianne Pastana de Sousa Miranda Daniel Pereira Santana Josefa Grasiela Silva Ramos Helaine Christine Cancela Pereira Messias Gonzaga 《Genetic Resources and Crop Evolution》2021,68(8):3333-3343
Genetic Resources and Crop Evolution - The development of segregating populations in papaya allows the development of new high fruit yield and excellent fruit quality pure line or hybrid cultivars.... 相似文献
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Claudia Simontacchi E. Negrato M. Pazzaglia D. Bertotto C. Poltronieri G. Radaelli 《Aquaculture International》2009,17(1):7-14
In general little is known about hormones and the ontogeny of the stress response in the early developmental stages of chondrostean
fishes and in particular of white sturgeon (Acipenser transmontanus, Richardson 1836). In this study, we measured for the first time cortisol and sex steroids (testosterone and estradiol) in
eggs, larvae, post-larvae, and fry of white sturgeon by radioimmunoassay (RIA), to elucidate some endocrine aspects of its
development. The cortisol, testosterone, and 17β-estradiol of maternal origin found in unfertilized eggs of white sturgeon
probably regulate both growth and development of the embryo. Cortisol decreased after fertilization, whereas testosterone
and 17β-estradiol did not significantly change. During the late stages of embryo development and immediately after hatching,
endogenous production of cortisol and sexual steroids, respectively, occurred. Sex steroids may be physiological inducers
of gonad sex differentiation in sturgeon. All steroids showed an increase 10 days post-hatch (dph), near the transition from
an endogenous to an exogenous energy source. Cortisol maintained the same basal levels even after metamorphosis, whereas testosterone
and 17β-estradiol declined significantly in post-larvae at 35 and 45 days post-hatch. In addition, to evaluate the ontogeny
of a functional hypothalamic-pituitary-interrenal (HPI) axis, larvae and fry were submitted to acute stress. The HPI axis
did not seem to be functional on the first day post-hatch, but became so from the third day post-hatch onward. 相似文献
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Gallina G. Poltronieri C. Merlanti R. De Liguoro M. 《Veterinary research communications》2008,32(1):287-290
Veterinary Research Communications - 相似文献
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Effects of dietary soy isoflavones on estrogenic activity,cortisol level,health and growth in rainbow trout,Oncorhynchus mykiss 
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下载免费PDF全文 Maria Rita Pastore Elena Negrato Carlo Poltronieri Giuseppe Barion Maria Messina Francesca Tulli Cristina Ballarin Lisa Maccatrozzo Giuseppe Radaelli Daniela Bertotto 《Aquaculture Research》2018,49(4):1469-1479
Soy isoflavones (the phytoestrogens genistein, daidzein and glycitein) may act as estrogen receptor agonists or antagonists. The aim of this study was to examine the effect of dietary isoflavones on growth, reproduction and health in rainbow trout (Oncorhynchus mykiss). Rainbow trout fed three experimental diets containing different concentrations of isoflavones (0, 500 and 1,500 ppm). Growth, estrogenic activity, plasma estradiol levels, gonadal development, state of stress and histological changes in selected tissues were evaluated at the end of 70 days. Neither growth performance nor the relative mRNA levels of Insulin Growth Factor I (igf‐I) in the liver were influenced by different levels of dietary isoflavones. Plasma and liver vitellogenin (VTG) protein levels and plasma 17‐β‐estradiol (E2) were unaffected by treatments, although the correlation between plasma levels of E2 and VTG densitometry values was significant (p < .05). The fish gonadosomatic index (GSI) did not significantly differ among the three experimental groups but correlated with plasma VTG densitometry values (p < .05). Plasma, muscle and fin cortisol concentrations fell within the normal welfare range and were not correlated with isoflavone levels. Histologically, the distal intestine showed a normal morphology with well‐differentiated enterocytes and in the liver hepatocytes were also normal. A supranuclear accumulation of lipid droplets in enterocytes and some lipid droplets in hepatocytes were observed in all tested groups, suggesting an impact of basal dietary lipid on transport/metabolism of fat in the fish. Overall, the present results suggest that the doses of isoflavones tested do not compromise rainbow trout reproduction, growth and health. 相似文献
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