Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus   总被引：2，自引：0，他引：2  

de Arriba ML  Carvajal A  Pozo J  Rubio P 《Veterinary immunology and immunopathology》2002,85(1-2):85-97

Eleven-day-old conventionally reared piglets were inoculated orally with two different doses of the cell-culture adapted strain CV-777 of the porcine epidemic diarrhoea virus (PEDV) or the virulent isolate of the same strain and challenged with the same virulent PEDV 3 weeks later. Pigs inoculated with the two doses of the attenuated virus did not show any typical sign of the disease, and virus shedding was not frequent. In contrast, 31% of pigs exposed to the virulent PEDV developed diarrhoea and virus shedding was demonstrated in 100%. At different postinoculation day (PID) and postchallenge day (PCD) virus-specific antibody-secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (blood and spleen) were assessed by enzyme-linked immunospot (ELISPOT). Only a small response was detected in the groups inoculated with attenuated PEDV, whereas in the group previously exposed to the virulent virus on PID 21 a large number of IgG and IgA ASC was detected. Isotype-specific antibody responses in serum were investigated by ELISA. IgG responses were detected in all groups, although the highest response corresponded to the group inoculated with virulent virus and only this group showed an IgA response. The pigs exposed to virulent PEDV were completely protected against the challenge with a higher dose of the same virulent virus on PID 21 and none of them shed the virus. The pigs inoculated with the attenuated strain were partially protected against the challenge, and 25% of the low dose- and 50% of the high dose-exposed pigs did not shed virus after challenge. All the pigs from a control group, not previously exposed to the virus, excreted the virus in faeces. A strong positive correlation was established between protection and the ASC responses detected in gut associated lymphoid tissues and blood at the challenge day and also between protection and serum isotype-specific antibody titers on that day. In addition, the IgA and IgG ASC responses detected in the blood on PID 21 also correlated with the responses found in the gut associated lymphoid tissues. The ASC and serum antibody responses after the challenge corresponded to a secondary immune response in the groups inoculated with attenuated virus, whereas a primary response was evident in the control group. No increase was seen in any of the parameters studied in the pigs inoculated with virulent PEDV.  相似文献   

Isotype-specific antibody-secreting cells in systemic and mucosal associated lymphoid tissues and antibody responses in serum of conventional pigs inoculated with PEDV     

de Arriba ML  Carvajal A  Pozo J  Rubio P 《Veterinary immunology and immunopathology》2002,84(1-2):1-16

An enzyme-linked immunospot (ELISPOT) has been developed to detect porcine epidemic diarrhea virus (PEDV)-specific antibody secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (spleen and blood) of conventional pigs so as to characterise the mucosal and systemic antibody response generated by the infection with PEDV. A total number of 28 eleven-day-old conventional pigs were orally inoculated with the field isolate of the PEDV strain CV-777. Diarrhea was observed in 32% of the pigs and virus shedding was demonstrated in 100% between postinoculation day (PID) 1 and 8. Serum IgG and IgA antibodies to PEDV were detected by isotype ELISA from PID 12 and 15, respectively, reaching maximum values at PID 32 (IgG) and 21 (IgA). PEDV specific IgM ASC occurred in all the tissues between PID 4 and 7, with the strongest response in the intestinal lamina propria. IgA and IgG ASC responses were evident in the intestinal lymphoid tissues from PID 21, the highest number of specific ASC corresponded to the duodenum lamina propria. In the systemic lymphoid tissues the number of IgG and IgA ASC detected were lower than in the mucosal tissues, however, in the blood, presence of IgA ASC was constantly detected from PID 14 until the end of the experiment. Memory antibody response to the PEDV was also studied by secondary in vitro stimulation of the mononuclear cells (MNC) isolated from mesenteric lymph nodes, spleen and blood. The memory B cell response was prominent at PID 21 and 25 and consisted in IgG and IgA ASC. To our knowledge, this is the first report to research into the presence and distribution of specific ASC in different locations of the systemic and the gut associated lymphoid tissues after a PEDV infection as well as the presence of memory B cells.  相似文献   

Bird-mediated effects of pest control services on crop productivity: a global synthesis     

D&#;az-Siefer  Pablo  Olmos-Moya  Natalia  Font&#;rbel  Francisco E.  Lavandero  Blas  Pozo  Roc&#;o A.  Celis-Diez  Juan L. 《Journal of pest science》2022,95(2):567-576

Journal of Pest Science - Birds provide important ecosystem services in many ecosystems, including important pest control effects on productive systems. The typically low bird diversity observed in...  相似文献   

Parallel testing of plasma iron and fibrinogen concentrations to detect systemic inflammation in hospitalized horses     

Ignacio Corradini DVM  MSc  Lara Armengou DVM  PhD  DECEIM  Judit Viu DVM  MSc  Maria L. Rodríguez‐Pozo DVM  Carla Cesarini DVM  MSc  DACVIM  DECEIM  Eduard Jose‐Cunilleras DVM  PhD  DACVIM 《Journal of Veterinary Emergency and Critical Care》2014,24(4):414-420

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Variability of herbaceous productivity along Nothofagus pumilio forest-open grassland boundaries in northern Chilean Patagonia     

L. Sánchez-Jardón  B. Acosta-Gallo  A. del Pozo  M. A. Casado  C. Ovalle  J. M. de Miguel 《Agroforestry Systems》2014,88(3):397-411

In order to develop a general model of aboveground net primary production (ANPP) of herbaceous communities in grazing systems that combine forested and open grasslands in temperate areas, biomass production and a set of biotic and abiotic variables were measured at four adjacent forest and grassland sites in Chile’s northern Patagonia for two consecutive growing seasons. At each site, one transect of 80 m long (40 m in open grassland and 40 m in forest) × 10 m wide was established. ANPP was significantly higher in open grasslands but no gradual change in biomass production was observed from inside the forest towards the open grassland. In open grasslands ANPP was spatially uniform but highly variable between years of contrasting weather conditions, whereas in forests it was more spatially heterogeneous and less variable over time. ANPP was highly correlated with cattle consumption. Structural equation models developed for the whole system confirm that ANPP was driven mainly by photosynthetically active radiation (PAR) and available nitrogen. However, we found important differences between forests and the adjacent open grasslands. In forests ANPP was enhanced by positive feedbacks between the amount of transmitted PAR through the canopy and soil nutrient input via cattle dung deposition. In open grasslands nitrogen availability appeared to be the main limiting factor but also influenced by weather conditions (dryer or wetter years). The coexistence of forests and grasslands patches, with different susceptibility of ANPP to meteorological and soil nutrient availability, highlights the importance of implementing an integrated silvopastoral system with lenga (Nothofagus pumilio [Poepp. & Endl.] Krasser) in northern Patagonia.  相似文献   

Identification of H3N2 influenza virus isolated from pigs with respiratory problems in Spain     

J M Castro  M del Pozo  I Simarro 《The Veterinary record》1988,122(17):418-419

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The antihistaminic chlorpheniramine inhibitsin vitro growth of several fungi isolated from harvested fruits     

Torés  Juan Antonio  González  María Dolores  Pozo  Eva  Urdiales  José Luis  Medina  Miguel Ángel  Sánchez-Jiménez  Francisca 《Phytoparasitica》1999,27(3):207-213

Chlorpheniramine (CPA) is an antihistaminic that changes the conformation of DNA and inhibits polyamine biosynthesis in mammalian cells. In the present work, we tested the effect of CPA on four genera of fungi species (Altemaria alternata, Botrytis cinerea, Cladosporium cladosporioides and threePenicillium spp.) grownin vitro. Similar growth inhibitions of these genera were produced by 0.5 raM iprodione, CPA and histidinol, but CPA was the most effective. The CPA sensitivities of the twoB. cinerea strains were different. Putrescine did not restore the fungal growth inhibited by CPA.  相似文献   

Ultrastructural analysis of sequential cyprinid herpesvirus 3 morphogenesis in vitro          下载免费PDF全文

S J Monaghan  S M Bergmann  K D Thompson  L Brown  T Herath  J del‐Pozo  A Adams 《Journal of fish diseases》2017,40(8):1041-1054

Cyprinid herpesvirus 3 (CyHV‐3) is an alloherpesvirus, and it is the aetiological agent of koi herpesvirus disease. Although the complex morphogenic stages of the replication cycle of CyHV‐3 were shown to resemble that of other members of the Herpesvirales, detailed analysis of the sequence and timing of these events was not definitively determined. This study describes these features through a time course using cyprinid cell cultures (KF‐1 and CCB) infected with CyHV‐3 (KHV isolate, H361) and analysed by transmission electron microscopy. Rapid viral entry was noted, with high levels of intracellular virus within 1–4 h post‐infection (hpi). Intranuclear capsid assembly, paracrystalline array formation and primary envelopment of capsids occurred within 4 hpi. Between 1 and 3 days post‐infection (dpi), intracytoplasmic secondary envelopment occurred, as well as budding of infectious virions at the plasma membrane. At 5–7 dpi, the cytoplasm contained cytopathic vacuoles, enveloped virions within vesicles, and abundant non‐enveloped capsids; also there was frequent nuclear deformation. Several morphological features are suggestive of inefficient viral assembly, with production of non‐infectious particles, particularly in KF‐1 cells. The timing of this alloherpesvirus morphogenesis is similar to other members of the Herpesvirales, but there may be possible implications of using different cell lines for CyHV‐3 propagation.  相似文献   

Grasslands in ‘Old World’ and ‘New World’ Mediterranean‐climate zones: past trends,current status and future research priorities          下载免费PDF全文

C. Porqueddu  S. Ates  M. Louhaichi  A. P. Kyriazopoulos  G. Moreno  A. del Pozo  C. Ovalle  M. A. Ewing  P. G. H. Nichols 《Grass and Forage Science》2016,71(1):1-35

Despite their ecological, economic and social importance, grasslands in areas with Mediterranean climates continue to receive limited scientific, political and media attention. The main objectives of this review are to compare and contrast dryland grasslands in the ‘Old World’ regions of the Mediterranean basin (southern Europe, western Asia and North Africa) with those of ‘New World’ regions with Mediterranean climates (Australia and Chile) and to identify common research priorities. The common characteristics and differences in climate, soils, native vegetation, importance of the livestock sector and the socio‐economic background for the different Mediterranean environments are examined. Past trends and the current status of temporary and permanent Mediterranean grasslands are also described. Some common issues between these regions are as follows: (i) adaptation to climate change; (ii) increasing persistence and drought survival of both annual and perennial species; (iii) the important role of forage legumes; (iv) maintaining grassland plant diversity; and (v) improved ecosystem services, such as carbon sequestration, control of soil erosion and wildfires, and preservation of both wild and domestic biodiversity. The favourable climate in these regions, which allows year‐round grazing and the growth of legumes, should be exploited to improve the sustainability of grassland‐based, extensive farming systems and the quality of their animal products, while at the same time improving ecosystem services. The decreasing support for grassland research and development programmes requires increased international scientific and technical cooperation among the few institutions operating in the different Mediterranean‐climate areas of the World to provide innovative and sustainable solutions to farmers.  相似文献   

Nuclear Configuration, Spindle Morphology and Cytoskeletal Organization of In Vivo Maturing Horse Oocytes     

MAR Siddiqui  EL Gastal  JC Ju  MO Gastal  MA Beg  OJ Ginther 《Reproduction in domestic animals》2009,44(3):435-440

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