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L. De Benedetti G. Burchi A. Mercuri N. Pecchioni P. Faccioli T. Schiva 《Plant Breeding》2000,119(5):443-445
Random amplified polymorphic DNA (RAPD) markers were used to verify interspecific hybridization in Alstroemeria. Five putative interspecific hybrids and their parents were analysed by means of four preselected RAPD primers. The putative parentage was confirmed in four hybrids and was excluded in one that showed completely different RAPD patterns from its putative parents and a different phenotype. Our results demonstrated that this molecular technique is a powerful tool for verifying hybridity rapidly if the putative parents are given. This tool will allow screening of small immature seedlings for verification of hybridity and should improve the efficiency of breeding programmes. 相似文献
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de Pinho Manzi M Nóbrega DB Faccioli PY Troncarelli MZ Menozzi BD Langoni H 《Research in veterinary science》2012,93(1):430-434
The aims of the present study were to relate intramammary infection (IMI) occurrence and somatic cell count (SCC) with teat-end condition (TEC) and udder cleanliness (UC). Milk samples from 1931 teats were evaluated according to the presence of IMI and SCC. Scores were applied to teats according to the TEC and to UC. Teats ends with a very rough ring had the largest number of IMI when compared to the other three categories, as well as animals with dirtier udders. The change in a TEC score increased by around 30% the chance of IMI. Also, the chance of the animal developing IMI increased by approximately 47% when the UC score increased. No significant association between both scores and quarter SCC was found. It can be concluded that animals with very rough teat end rings and very dirty udders have a greater predisposition to IMI. 相似文献
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F. G. Frantz L. N. Andrade R. Araújo J. I. Araújo L. H. Faccioli D. N. Sato 《Zoonoses and public health》2010,57(6):382-387
With the evidence showing the protection variability of bacille Calmette‐Guérin, new potential vaccines for tuberculosis have been tested around the world. One of the general concerns in tuberculosis vaccine development is the possibility of priming the host immune system with prior exposure to environmental mycobacteria antigens, which can change the efficacy of subsequent vaccination. As there is a great homology between the species from Mycobacterium genera, the previous contact of experimental animals with environmental mycobacteria could sensitize the mice and, in this way, could influence subsequent vaccine research. The aim of our study was to investigate critical points in an animal facility to search for environmental mycobacteria that eventually could be in direct or indirect contact with the experimental animals. Samples were collected from surfaces of walls, floor, animal cages and shelves and analysed using the Ogawa–Kudoh decontamination method. Samples of drinking water, food and sawdust were collected for analysis by the NALC/NaOH decontamination method. Also, the samples were cultivated directly in broth medium, without any method for decontamination. After decontamination methods, we observed bacterial colony growth in 4.31% of the total of samples analysed. These samples were stained with Ziehl–Neelsen and we did not detect any acid‐fast bacilli, suggesting that the animal facility analysed is free from contamination by environmental mycobacteria and is not a source of mycobacterial antigens. Furthermore, our study showed a new paradigm in tuberculosis vaccine development: concern about the animal facility environment in terms of immune system priming of experimental animals by nascent bacterial contaminants. 相似文献
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DNA-based methods for identification and quantification of small grain cereal mixtures and fingerprinting of varieties 总被引：1，自引：0，他引：1
Valeria Terzi Caterina Morcia Antonio Gorrini A. Michele Stanca Peter R. Shewry Primetta Faccioli 《Journal of Cereal Science》2005,41(3):213-220
The composition of cereal-based foods is a key factor in determining the quality and safety of the final product while the reliable identification of cereal species and cultivars are essential for the handling, marketing and processing of grain and for the protection of plant breeders' rights. Analytical methods have therefore been developed and applied to identify and quantify cereal species in food products and also to fingerprint and identify grain at the genotype and variety levels. DNA-based methods for the detection and quantification of mixtures of small grain cereals are reviewed, together with the recent development of molecular markers for varietal fingerprinting. 相似文献
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Raffaella Tudisco Federico Infascelli Monica Isabella Cutrignelli Fulvia Bovera Caterina Morcia Primetta Faccioli Valeria Terzi 《Livestock Science》2006,105(1-3):12-18
The effect of the digestion process in the gastro-intestinal tract (GIT) of animal models on the fate and integrity of plant DNA has been widely evaluated since DNA availability and integrity is a key factor for hypothetical horizontal gene transfer of recombinant DNA from GM crop-derived feeds to animal and human gut microflora. In this study, plant DNA sequences from high and low copy number genes were monitored in GIT and tissues of buffaloes and rabbits. Using a real-time PCR approach to track plant DNA in animal samples, we demonstrated the persistence of fragmented plant DNA blood and tissues of buffaloes and rabbits raised with conventional feeding. 相似文献
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Aline M. de Alcntara Flvio A. L. da Fonseca Thyssia B. Araújo‐Dairiki Claudemir K. Faccioli Carlos A. Vicentini Luís E. C. da Conceio Ligia U. Gonalves 《Journal of the World Aquaculture Society》2019,50(1):231-241
Early‐life survival of Arapaima gigas is one of the main challenges of its farming. In this study, we described the morphological and histochemical development of the gastrointestinal tract of arapaima larvae. Larvae were collected from a pond when they started to swim to the water surface (initial day) and were housed in indoor tanks. Daily samplings (n = 10) were performed from 0 to the 11th day after the collection (DAC) and then on the 14th, 17th, and 20th DAC. On the initial day, arapaima larvae (0.05 ± 0.01 g; 2.21 ± 0.06 cm) had opened mouth and anus and no yolk sac. In addition, larvae presented well‐developed digestive organs. Gastric glands were fully formed, with positive reactions to alcian blue (AB) pH 1.0 as well as to periodic acid‐Schiff (PAS) in the simple columnar epithelium. There were folds throughout the intestine and brush border, with an AB pH of 2.5 + PAS positive mucins. From 1 to 11 DAC, larvae presented increasing concentrations of gastric glands and thickness of the stomach muscular layer. From 14 to 20 DAC, the intestine presented high‐fold complexity. We suggest that arapaima larvae may be fed exogenous inert diets at a size of around 2 cm. 相似文献
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Summary The potato leafroll virus (PLRV) coat protein (CP) gene was directly cloned from the total RNA extracted from virus-infected
plants. First strand cDNA synthesis was not necessarily specific; it was equally efficient using either random or CP-specific
primers. The viral sequence encoding the coat protein was specifically amplified from the total population of cDNA molecules
by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained
was cloned blunt-end into the pT7T318U plasmid vector, and the authenticity of the cloned gene verified by sequence analysis.
This cloning strategy obviates the need for virus purification. Sequence comparison of the CP gene of the Italian isolate
and those of five other PLRV isolates revealed a close similarity to the three European and the Canadian isolates, and a more
distant relationship with the Australian one. 相似文献
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Valeria Terzi Nicola Pecchioni Primetta Faccioli Ladislav Kučera Antonio Michele Stanca 《Genetic Resources and Crop Evolution》2001,48(5):447-458
Fifteen Hordeum species and subspeciesbelonging to groups with different genomes were studied usingPCR-based markers to establish phylogenetic relationshipswithin the genus. Two hundred and seventeen RAPD and STS markers wereused to calculate genetic distances and construct phylogenetic trees.The phenetic analysis clearly separated the primary gene pool,represented by H. vulgaressp. vulgare, H.vulgare ssp. vulgare convar. vulgare f. agriochriton andH. vulgare ssp.spontaneum, from the secondary gene pool,represented by H. bulbosumand the tertiary gene pool, represented by American wild barleys andH. bogdanii. Data obtainedfrom PCO analysis are in complete agreement with taxonomicclassifications proposed previously, which were comparisons ofnumerous morphological, cytological and reproductivecharacters. 相似文献