Evaluation the effect of subchronic feeding of transgenic cotton line (CKC1) on the faecal microbiota of albino rabbits     

Ibrahim Bala Salisu  Ahmad Ali Shahid  Amina Yaqoob  Ambreen Gul  Abdul Qayyum Rao 《Journal of animal physiology and animal nutrition》2021,105(2):354-363

Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host-microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty-eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real-time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log₁₀ copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long-term study.  相似文献   

Effects of Stocking Density and Monosex Culture of Freshwater Prawn Macrobrachium rosenbergii on Growth and Production in Concrete Tanks in Saudi Arabia     

Abdul Qayyum  Siddiqui  Yousef S.  Al-Hafedh  Ahmed H.  Al-Harbi Seikh A.  Ali 《Journal of the World Aquaculture Society》1997,28(1):106-112

Abstract This study was conducted to evaluate the effects of stocking density and monosex culture on growth, survival, yield and feed conversion ratio of freshwater prawn Macrobrachium rosenbergii in concrete tanks. Juvenile prawns with an average weight of 1.8 g were stocked into triplicate tanks at densities of 5, 10, 15 and 20 prawns/m², grown for 168 d and fed a 34% tilapia diet. Stocking density had significant effect on prawns. Final mean body weight decreased with the increasing density, being highest at 5 prawns/m² (29.6 9). and lowest at 20 prawns/m² (17.4 g). Total yield increased from 135 g/m² (1,350 kg/ha) at a density of 5 prawns/m² to 261 g/m² (2,610 kg/ha) at density 15/m^z to 245 g/m² (2,450 kg/ha) at 20/m^z. Feed conversion ratios were high and ranged from 3.7 (5 prawns/m²) to 5.6 (20 prawns/m²).
In monosex culture of freshwater prawns stocked in triplicate tanks at a density of 5 prawns/m² for 112 d, the all-male population had the best growth performance and feed conversion ratio, followed by the mixed-sex and all-female populations. The all-male population had 99% marketable prawns (>20 g) with an average yield of 159 g/m² (1,590 kg/ha); the mixed-sex population had 90% marketable prawns and the yield was 135 g/m^z (1,350 kg/ha); and the all-female population had 75% marketable prawns with an average yield of 108 g/m² (1,080 kgha).  相似文献   

Inhibition of tumorigenesis in ApcMin/+ mice by a combination of (-)-epigallocatechin-3-gallate and fish oil     

Bose M  Hao X  Ju J  Husain A  Park S  Lambert JD  Yang CS 《Journal of agricultural and food chemistry》2007,55(19):7695-7700

The effect of a combination of (-)-epigallocatechin-3-gallate (EGCG) with fish oil on intestinal tumorigenesis in Apc (Min/+) mice fed a high-fat diet was investigated in the present study. The combined treatment of EGCG and fish oil for 9 weeks reduced the tumor number by 53% as compared to controls while neither agent alone had a significant effect. Apoptosis was significantly increased in all treatment groups. beta-Catenin nuclear positivity in adenomas from the combination group was lower than control mice, implicating the modulation of Wnt signaling by the combination. Fish oil and the combination significantly reduced prostaglandin E 2 (PGE 2) levels in small intestinal tumors as compared to controls, suggesting modulation of aberrant arachidonic acid metabolism by fish oil. Akt phosphorylation in adenomas was significantly reduced in all treatment groups, which may have contributed to the observed increase in apoptosis. The results indicate that a combination of low doses of EGCG and fish oil can inhibit tumor multiplicity in Apc (Min/+) mice.  相似文献   

Decolorization of Textile Effluent by Soluble Fenugreek (Trigonella foenum-graecum L) Seeds Peroxidase     

Qayyum Husain  Zoheb Karim  Zeeshan Zahoor Banday 《Water, air, and soil pollution》2010,212(1-4):319-328

Peroxidase from fenugreek (Trigonella foenum-graecum) seeds was highly effective in the decolorization of textile effluent. Effluent was recalcitrant to the action of fenugreek seeds peroxidase (FSP). However, in order to effectively decolorize effluent by peroxidase, the role of six redox mediators has been investigated. The maximum decolorization of textile effluent was observed in the presence of 1.0 mM 1-hydroxybenzotrizole, 0.7 mM H₂O₂, and 0.4 U ml^?1 of FSP in the buffer of pH 5.0 at 40°C in 2.5 h. The decolorization of textile effluent in a batch process by peroxidase was 85% in 5 h, whereas the complete decolorization of textile effluent by membrane-entrapped FSP was observed within 11 h of its operation. The absorption spectra of treated effluent exhibited a marked diminution in the absorbance at different wavelengths compared to untreated effluent.  相似文献   

Crystal structure analysis of deamino-oxytocin: conformational flexibility and receptor binding   总被引：1，自引：0，他引：1  

S P Wood  I J Tickle  A M Treharne  J E Pitts  Y Mascarenhas  J Y Li  J Husain  S Cooper  T L Blundell  V J Hruby 《Science (New York, N.Y.)》1986,232(4750):633-636

Two crystal structures of deamino-oxytocin have been determined at better than 1.1A resolution from isomorphous replacement and anomalous scattering x-ray measurements. In each of two crystal forms there are two closely related conformers with disulfide bridges of different chirality, which may be important in receptor recognition and activation.  相似文献   

Age of a lunar anorthosite     

Husain L  Schaeffer OA  Sutter JF 《Science (New York, N.Y.)》1972,175(4020):428-430

The crystallization age of an Apollo 15 anorthosite rock, 15415,9, returned from the lunar highlands has been measured to be (4.09 +/- 0.19) x 10(9) years. The primitive lunar crust must have been formed in the first 300 to 400 x 10(6) years. The results give some credence to the hypothesis that the primitive lunar surface was molten and large-scale fractional crystallization occurred in the early history of the moon.  相似文献   

Dynamic shifts of limited working memory resources in human vision     

Bays PM  Husain M 《Science (New York, N.Y.)》2008,321(5890):851-854

Our ability to remember what we have seen is very limited. Most current views characterize this limit as a fixed number of items-only four objects-that can be held in visual working memory. We show that visual memory capacity is not fixed by the number of objects, but rather is a limited resource that is shared out dynamically between all items in the visual scene. This resource can be shifted flexibly between objects, with allocation biased by selective attention and toward targets of upcoming eye movements. The proportion of resources allocated to each item determines the precision with which it is remembered, a relation that we show is governed by a simple power law, allowing quantitative estimates of resource distribution in a scene.  相似文献   

Comment on "Detecting awareness in the vegetative state"     

Nachev P  Husain M 《Science (New York, N.Y.)》2007,315(5816):1221; author reply 1221

Owen et al. (Brevia, 8 September 2006, p. 1402) claimed that a patient's brain activity revealed that she was consciously responding to commands despite being in a vegetative state. However, several alternative explanations were not eliminated. Specifically, the activity could reflect unconscious reactions to the last word in the command, not conscious decisions to respond. A refined experimental design could clarify these issues.  相似文献   

Growth,survival, and heavy metal (Cd and Ni) uptake of spinach (Spinacia oleracea) and fenugreek (Trigonella corniculata) in a biochar‐amended sewage‐irrigated contaminated soil          下载免费PDF全文

Uzma Younis  Muhammad Farooq Qayyum  M. Hasnain Raza Shah  Subhan Danish  Ahmad Naeem Shahzad  Saeed Ahmad Malik  Seema Mahmood 《植物养料与土壤学杂志》2015,178(2):209-217

Irrigation of arable land with contaminated sewage waters leads to the accumulation of trace metals in soils with subsequent phyto‐/zootoxic consequences. In this study, biochar derived from cotton sticks was used to amend an agricultural silt‐loam soil that had been previously irrigated with trace metal contaminated sewage waters. Metal accumulation and toxicity to spinach (Spinacia oleracea) and fenugreek (Trigonella corniculata) was investigated by measuring concentrations of Cd and Ni in plant tissues and various photosynthetic and biochemical activities of plants. Positive impacts of biochar on both spinach and fenugreek were observed in terms of biomass production that increased from 29% to 36% in case of spinach, while for fenugreek this increase was 32% to 36%. In the control treatment there was an increase in malondialdihyde, soluble sugar, and ascorbic acid contents, indicating heavy metal stress. Biochar applications increased soluble proteins and amino acids in plants and reduced the uptake of Cd from 5.42 mg kg^?1 at control to 3.45 mg kg^?1 at 5% biochar amended soil and Ni (13.8 mg kg^?1 to 7.3 mg kg^?1 at 5% biochar) by the spinach plants. In fenugreek, the Cd was reduced from 7.72 mg kg^?1 to 3.88 mg kg^?1 and reduction in Ni was from 15.45 mg kg^?1 to 9.46 mg kg^?1 at 5% biochar treated soil, reducing the possibility of transfer up the food chain. This study demonstrates that the use of biochar made from cotton‐sticks, as an amendment to arable soils that have received contaminated irrigation water, could improve plant growth and decrease Cd and Ni uptake to crops, alleviating some of the negative impacts of using sewage waters on arable land.  相似文献   

Proton magnetic resonance spectroscopic method for determination of phenylbutazone and oxyphenbutazone in tablets     

S Husain  M Kifayatullah  R N Rao 《Journal of the Association of Official Analytical Chemists》1988,71(3):525-527

A simple, specific, and rapid 1H nuclear magnetic resonance spectroscopic method for the assay of phenylbutazone and oxyphenbutazone is described. Spectra are recorded in CDCI3 containing 1,3-dichloro-5-nitrobenzene as an internal standard. The aromatic proton resonances for the standard, at delta 7.7 and 8.2, are well separated from those of phenylbutazone and oxyphenbutazone, which are in the region of delta 6.5-7.3 ppm. Average percent recoveries of phenylbutazone and oxyphenbutazone were 98.9 and 98.6 with standard deviations of 0.6 and 0.7, respectively. Commercial formulations were analyzed and the results obtained by the proposed method closely agreed with those found by the British Pharmacopoeia method.  相似文献