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Fares R Bazzi S Baydoun SE Abdel-Massih RM 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):58-63
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study,
the anti-proliferative activity of plant extracts from olive (Olea europaea L.) leaves was tested on human leukemic cell line (Jurkat). Cytotoxicity of various concentrations of plant extracts was examined
and the IC50 was determined. Olive leaf extracts showed concentration-dependent anti-proliferative effect as determined by the WST-1 proliferation
kit and [3H]-thymidine incorporation method. To study whether cell death was due to apoptosis, cells were stained with Annexin V-FITC
and PI and the expression of important regulatory proteins (Bcl-2, Bax, and p53) involved in apoptosis were examined by Western
blot. The antioxidant activity of olive leaves (SC50 = 0.1 mg dry weight) was studied using the DPPH scavenging method. Present findings suggest that olive leaves extracts exhibit
anti-proliferative effect on leukemic cells by inducing apoptosis. 相似文献
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Jamal Sawwan Rida A. Shibli Ihsan Swaidat Monther Tahat 《Journal of plant nutrition》2013,36(6):759-771
Interactive effects of phosphorus (P) with in vitro‐induced water deficit (using sorbitol and mannitol) were studied on African violet (Saintpaulia ionantha) whole‐plant microculture. Sorbitol and mannitol significantly reduced (more negative) the cell sap osmotic potential. Increased P was very effective in increasing (less negative) the osmotic potential of cell sap under the imposed water deficit treatments. On the other hand, induced water deficit significantly reduced shoot growth (shoot height and dry mass), and root number and length, whereas P mitigated these adverse effects and improved shoot and root growth. Cultures exposed to water deficit at 150mM sorbitol and mannitol experienced some physiological disorders (about 10% shoot tip browning, 15% stem basal‐end browning) and 20% chlorosis. Physiological disorders and chlorosis were totally mitigated with increased P to 1.0 or 2.0 mM. Phosphorus concentration in shoot tissues was decreased with water deficit in the medium and enhanced by elevated P in the medium. Ex vitro survival percentages increased in plantlets that experienced in vitro water deficit with sorbitol and mannitol at 50 to 100 mM and P at 1.0 to 2.0 mM. We conclude that P is a key factor to regulate cell osmotic potential and growth under in vitro induced water deficit. On the other hand, microculture level is a very effective alternative for the study of plant response and tolerance to water deficit and its interaction with nutrient availability in the root zone. 相似文献
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The effects of application method and concentration of gibberellic acid (GA3), paclobutrazol and chlormequat on black iris performance were assessed. Plants (10 cm high, 4 ± 1 leaves) were sprayed with 125, 250, 375 or 500 mg L−1 or drenched with 0.25, 0.5, 1 or 2 mg L−1 GA3. In a second experiment, the plants were sprayed with 100, 250, 500 or 1000 mg L−1 or drenched with 0.25, 0.5, 1 or 2 mg L−1 paclobutrazol. Other plants were sprayed with 250, 500, 1000 or 1500 mg L−1 or drenched with 100, 250, 375 or 550 mg L−1 chlormequat. In each experiment, the control treatment consisted of untreated plants. Results indicated that the tallest plants (37.3 cm) in the GA3 experiment were those sprayed with 250 mg L−1. The most rapid flowering (160 days after planting) occurred when a 375 mg L−1 GA3 spray was used, whereas flowering was delayed to 200 days using 1 mg L−1 GA3 drench. Drenching with 1 mg L−1 GA3 increased height of the flower stalk by 7 cm compared to the control. Though relatively slow to flower, plants drenched with 1 mg L−1 GA3 had long and rigid stalks, which were suitable as cut flowers. Number and characteristics of the sprouts were not affected by GA3. All paclobutrazol sprays resulted in leaf falcation. A 500 or 1000 mg L−1 paclobutrazol spray resulted in severe and undesirable control of plant height, drastic reduction in stalk height and weight, and delayed flowering. Plants drenched with 0.25 or 1 mg L−1 paclobutrazol were suitable as pot plants. Chlormequat reduced plant height only at the highest drench concentration, which also reduced flowering to 70%. No leaf falcation was observed with GA3 or chlormequat. Chemical names: ( ± )-(R*,R*)-beta-((4-chlorophenyl)methyl)-alpha-(1,1,-dimethylethyl)-1H-1,2,4,-triazol-1-ethanol (paclobutrazol); (2-chloroethyl) trimethylammonium chloride (chlormequat). 相似文献
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Safwan M. Shiyab Mohamad A. Shatnawi Rida A. Shibli Nihad G. Al Smeirat Jamal Ayad Muhanad W. Akash 《Journal of plant nutrition》2013,36(4):665-676
□ Growth and nutrient acquisition of tomato (Lycopersicon esculentum L.) cv ‘Amani’ were studied under induced salt stress in Hoagland's solution. The plants were treated for 37 days with salinity induced by incorporating different concentrations [0.0 (control), 50, 100, 150, or 200 mM] of sodium chloride (NaCl) to the nutrient solution. Slight reduction was obtained in growth represented by (shoot length and number, leaf number, and dry weight) when seedlings were directly exposed to NaCl stress from 0.0 to 100 mM. At higher concentrations (150 or 200 mM), growth parameters were adversely affected and seedlings died thereafter. Elevated salinity significantly reduced crude protein and fiber in shoots and roots. Tomato shoot and root contents of potassium (K), iron (Fe), and ash were reduced significantly in response to increased levels of salinity. Tissue contents of sodium (Na) and chloride (Cl) increased with elevated salinity treatments. 相似文献
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Rida Shibli Munir Mohammad Abdullah Abu‐Ein Mohammad Shatnawi 《Journal of plant nutrition》2013,36(9):1209-1215
Growth and micronutrient acquisition of some apple (Malus dometsica Borkh) varieties (Galla, MM 111, MM I06, M 26, and M6 York) were studied under gradually in vitro induced NaCl (from 0, 50 to 100 mM) salinity. Microshoot dry weight tended to increase with increasing salinity level in all varieties except in M 26 and M6 York as some decline was obtained at 100 mM NaCl. Shoot proliferation was decreased significantly in Galla at 100 mM NaCl and at both salinity levels in M 26 compared to the control (0 mM). A significant decrease in shoot zinc (Zn) acquisition with increasing salinity level was obtained in all varieties except MM 111. M6 York and MM 111 decreased their copper (Cu) acquisition significantly at 50 mM NaCl and then increased at 100 mM salinity level. Meanwhile, a significant decrease in Cu acquisition with increasing NaCl concentration was obtained in Galla and M 26. Galla, MM 106, and M 26 showed a significant increase in manganese (Mn) acquisition at 50 mM NaCl followed by a decrease at 100 mM salinity level. On the other hand, Mn acquisition continued to decrease significantly in MM 111 while M6 York increased its Mn acquisition with increasing salinity. M 111 and M6 York showed a significant decrease in iron (Fe) acquisition at 50 mMNaCl followed by an increase at 100mM NaCl. Galla and M 26 continued to decrease Fe acquisition with elevated salinity level, whereas Fe in MM 106 increased with salinity. 相似文献
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Ameer Muhammad Wajahat Jabeen Farhat Asad Muhammad Kaukab Ghazala Bashir Amnah Rasheed Misha Younis Hafsa Munir Naveed Nawaz Javaria Zainab Rida Akram Muhammad 《Fish physiology and biochemistry》2021,47(5):1559-1564
Fish Physiology and Biochemistry - The applications of exogenous hormones in different species for the induction of oocyte production, final oocyte maturation (FOM), and spawning for their... 相似文献
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The growth and production of miniature dwarf tomato selection Lycopersicon esculentum ’Micro‐Tom’ plants grown from seedling to harvest in solution batph culture’ at four different NaCl salinity levels (2.4 [control, no NaCl], 7.6, 12.8, or 18 dS‐m‐1 solution conductivities) was monitored. Incremental reductions in canopy extent and shoot area of ‘Micro‐Tom’ were observed with increasing solution NaCl level. Root growth and shoot height were somewhat less responsive to imposed salinity. Fruit number, fruit size, and leaf tissue osmotic potential decreased as NaCl concentration increased. Fruit yield was highly correlated with total canopy and shoot area, but not with tissue osmotic or total water potential. ‘Micro‐Tom’ plants survived and continued fruit production at higher salinity levels despite reduced canopy growth. Treatment effects on vegetative growth and fruit production became more pronounced later in the growth cycle. 相似文献
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Nabila S. Karam Khalil I. Ereifej Rida A. Shibli Hani AbuKudais Ahmad Alkofahi Yahyah Malkawi 《Journal of plant nutrition》2013,36(4):725-739
In vitro plantlets or microtubers (in vitro produced tubers) of ‘Spunta’ potato (Solanum tuberosum L.) were planted in a 3 soil: 2 peat moss: 1 sand substrate (by volume) amended with municipal solid waste (MS W) compost at 0, 10, 20, or 30 g 4‐1 L pot. Three months later, plant growth and tuber yield were evaluated and concentrations of shoot and tuber tin (Sn), arsenic (As), copper (Cu), zinc (Zn), nickel (Ni), lead (Pb), manganese (Mn), cadmium (Cd), and iron (Fe) were determined. Amending with MSW resulted in significant increases in concentrations of all tested metals in the substrate. Number of proliferated shoots of plants started from rooted plantlets was greatest at 10 g pot‐1 MSW, whereas shoot weight of plants started from microtubers was greatest at 10 and 20 g pot‐1 MSW. Tuber yield of plants started from rooted plantlets or microtubers was greatest at 10 or 30 g pot‐1 MSW, respectively. In all instances, amending with MSW at 30 g pot‐1 resulted in significant increases in concentrations of all tested metals in shoots and tubers. Concentrations of shoot Ni and tuber Zn and Fe for plants started from rooted plantlets and concentrations of shoot Fe and tuber As, Cu and Pb for plants started from microtubers increased consistently with increasing MSW percentage of the substrate. Plants started from rooted plantlets produced shoots with sufficient Zn, Mn, and Ni concentrations regardless of the substrate but with toxic Cu content at 30 g pot‐1 MSW. Plants started from microtubers produced shoots with sufficient Mn and Ni concentrations regardless of the substrate but with low Zn and deficient Cu in unamended substrates. All plants had shoot Fe content higher than the sufficiency range. Although there were significant differences in concentrations of some nutrients among MSW treatments, no symptoms of nutrient toxicity or deficiency were observed. In all instances, tested elements did not accumulate in tubers to levels hazardous to human health. Concentrations of Cd, the most hazardous element, in potato tubers was not high enough to pose a threat to human. Our results indicate that there is a potential use of MSW in satisfying the needs of potato growth with negligible increases in heavy metal concentrations in tubers. 相似文献
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In vitro and hydroponic intercropping systems were compared to examine the biocompatibility responses between ‘Fiona F1’ tomato (Lycopersicon esculentum Mill.) and ‘Spunta’ potato (Solanum tuberosum). The biological response of monocultures (with four plantlets per vessel) of each species (including change in medium electrical conductivity (EC), osmotic potential (OS), and tissue mineral element content) were directly compared to intercropped cultures of the following tomato:potato; 1:1, 2:1, 3:1, 1:2, 1:3, and 2:2 plantlets per vessel. The in vitro intercropping systems were performed using a Murashige and Skoog (MS) shoot proliferation medium (5.0 μM BA and 0.5 μM IBA). The parallel hydroponic intercropping system was performed using a Hoagland's solution. Monoculture of both species grows better in both systems than intercropped species. In the intercropped vessels, tissue mineral content decreased, medium pH became more acidic, OS decreased and EC increased as compared to monocultures in parallel vessels. A lettuce seedling bioassay did not indicate evidence of allelopathic compound accumulation in either hydroponic or in vitro intercropped systems. 相似文献