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Nitric acid (HNO3) vapor is a significant component of air pollution. Dry deposition of HNO3 is thought to be a major contributor to terrestrial loading of anthropogenically-derived nitrogen (N), but many questions remain regarding the physico-chemical process of deposition and the biological responses to accumulation of dry-deposited HNO3 on surfaces. To examine these processes experimentally, a continuously stirred tank reactor (CSTR) fumigation system has been constructed. This system enables simultaneous fumigation at several concentrations in working volumes 1.3 m dia by 1.3 m ht, allowing for simultaneous fumigation of many experimental units. Evaluation of the system indicates that it is appropriate for long-term exposures of several months duration and capable of mimicking patterns of diurnal atmospheric HNO3 concentrations representative of areas with different levels of pollution.  相似文献   
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Symptoms resembling off-target plant growth regulator (PGR) herbicide injury are frequently found in soybean fields, but the causal agent is often difficult to identify. The expression of GH3, an auxin-regulated soybean gene, was quantified from soybean leaves injured by PGR herbicides using real-time RT-PCR. Expression of GH3 was analyzed to ascertain its suitability for use in a diagnostic assay to determine whether PGR herbicides are the cause of injury. GH3 was highly induced by dicamba within 3 days after treatment (DAT) and remained high at 7 DAT, but induction was much lower at 17 DAT. GH3 was also highly induced at 7 DAT by dicamba + diflufenzopyr, and to a lesser extent by the other PGR herbicides clopyralid and 2,4-D. The non-PGR herbicides glyphosate, imazethapyr, and fomesafen did not significantly induce GH3 expression above a low constitutive level. These results indicate that a diagnostic assay for PGR herbicide injury based on overexpression of auxin-responsive genes is feasible, and that GH3 is a potential candidate from which a diagnostic assay could be developed. However, time course analysis of GH3 expression indicates the assay would be effective for a limited time after exposure to the herbicide.  相似文献   
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Waterhemp (Amaranthus tuberculatus) is a common and troublesome weed in cropping systems throughout the United States. With the potential for future periods of low rainfall or drought, the need for improved weed control under drought stress is necessary. Drought stress typically reduces herbicide efficacy by reducing the foliar uptake of herbicides and their translocation. The objectives of this research were to determine the efficacy of 2,4‐dichlorophenoxyacetic acid (2,4‐D) and glyphosate, applied alone or when tank‐mixed, on waterhemp under varying levels of drought stress, the effect of the timing of drought stress in relation to herbicide application and the absorption and translocation of each herbicide in drought‐stressed waterhemp. At reduced herbicide rates, 2,4‐D had a greater level of control of waterhemp under drought stress, compared to glyphosate. The level of herbicide efficacy was lower when the amount of water that was applied to the plants was reduced. The level of waterhemp control was greatest when drought stress occurred before the herbicide application and when the plants were watered to saturation after the application, compared to when drought stress occurred after the herbicide application or restricted watering levels occurred throughout the entire study. Glyphosate absorption and translocation were reduced in the drought‐stressed plants, but 2,4‐D absorption and translocation were not altered. The absence of a reduction in 2,4‐D translocation in the drought‐stressed weeds has not been previously reported. Applying herbicides prior to a rainfall event could increase the weed control level, even if the weed is stressed. Determining how and why 2,4‐D absorption and translocation levels, compared to those of glyphosate, are unaffected by drought stress in waterhemp can aid in improving the control of drought‐stressed weeds with other postemergence herbicides.  相似文献   
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BACKGROUND: A population of waterhemp in a seed maize production field in central Illinois, United States, was not adequately controlled after post‐emergence applications of herbicides that inhibit 4‐hydroxyphenylpyruvate dioxygenase (HPPD). RESULTS: Progeny from the field population survived following treatment with mesotrione, tembotrione or topramezone applied to the foliage either alone or in combination with atrazine in greenhouse experiments. Dose–response experiments indicated that the level of resistance to the HPPD inhibitor mesotrione is at least tenfold relative to sensitive biotypes. CONCLUSION: These studies confirm that waterhemp has evolved resistance to HPPD‐inhibiting herbicides. Copyright © 2011 Society of Chemical Industry  相似文献   
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Adjuvants are traditionally thought to exert their main effect on the cuticle or spray droplet to enhance foliar-applied herbicide penetration. However, considerable evidence exists indicating that the plasma membrane is a barrier to intracellular penetration of herbicides and a site of action for adjuvants. Surfactants may penetrate through the cuticle and into the region of the plasma membrane. Insertion into the membrane causes a general “ loosening” to provide greater penetration by highly polar herbicides such as glyphosate. Weak acid herbicides typically have a lipophilic moiety and, therefore, can move more easily through the membrane but the rate and accumulation is dependent on pH conditions across the membrane. Ammonium salts have been shown to affect the pH of the apoplast in a manner which allows faster penetration and greater accumulation of weak acid herbicides. Examination and understanding of the plasma membrane as a barrier to herbicide penetration will aid in defining the mechanisms of adjuvant action and improve the efficiency of agrochemical use.  相似文献   
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