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Peek SF Borah S Semrad S McGuirk S Slack JA Patton E Coombs D Lien L Darien BJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):29-31
Background: Accurate determination of plasma endotoxin concentration is critical for ex vivo and in vitro cellular and molecular studies of endotoxemia in horses. However, reports are conflicting with respect to anticoagulant, handling, and sample preparation.
Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses.
Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were spiked with endotoxin (2 EU/mL). Endotoxin-spiked WB samples were centrifuged immediately to generate PRP for measurement. Endotoxin concentration was subsequently measured by Limulus amebocyte assay at 0, 15, 30, 45, and 60 minutes. Assays were performed in triplicate and results were analyzed using Student's t -test, with significance set at P < .05.
Results: Mean endotoxin concentrations in 2 EU/mL-spiked WB were significantly different from those in PPP at all time points tested. Recovery of endotoxin in PRP generated from WB was significantly diminished after just 15 minutes.
Conclusion: PRP generated from WB is significantly more reliable than PPP in determining endotoxin concentration ex vivo. Measurement of endotoxin in PRP generated from WB was significantly diminished after 15 min, identifying a time frame within which to process blood samples for endotoxin analysis. 相似文献
Objective:
Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses.
Methods:
Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were spiked with endotoxin (2 EU/mL). Endotoxin-spiked WB samples were centrifuged immediately to generate PRP for measurement. Endotoxin concentration was subsequently measured by Limulus amebocyte assay at 0, 15, 30, 45, and 60 minutes. Assays were performed in triplicate and results were analyzed using Student's t -test, with significance set at P < .05.
Results:
Results: Mean endotoxin concentrations in 2 EU/mL-spiked WB were significantly different from those in PPP at all time points tested. Recovery of endotoxin in PRP generated from WB was significantly diminished after just 15 minutes.
Conclusion:
Conclusion: PRP generated from WB is significantly more reliable than PPP in determining endotoxin concentration ex vivo. Measurement of endotoxin in PRP generated from WB was significantly diminished after 15 min, identifying a time frame within which to process blood samples for endotoxin analysis. 相似文献
2.
Gaurav Mishra Rossana Marzaioli Krishna Giri Rinkumoni Borah Antara Dutta R.S.C. Jayaraj 《Archives of Agronomy and Soil Science》2017,63(10):1355-1368
The study aimed to evaluate the impact of shifting cultivation on soil quality, in Wokha district of Nagaland, using weighted soil quality index (SQIw). Four forest sites and three sites under shifting cultivation were selected and physical (texture and bulk density) and chemical (pH, electrical conductivity, cation exchange capacity, organic carbon, available N, P, K, exchangeable cations) parameters were analyzed. The SQIw was calculated on a minimum data set selected by principal component analysis, followed by varimax rotation algorithm and through subsequent normalization of the parameters using the linear scoring function. Significant (P < 0.05) variations in SQIw were found across the depth of the soil and land use according to severity of factors associated with land use. Three different classes of soil quality were also identified: high quality (SQIw > 0.70) for two forest soils (FS1 and FS2) and land under shifting cultivation (JH1), low quality (SQIw < 0.50), only to FS3, and intermediate quality (0.50 < SQI < 0.70) in all other soils. The approach used in this study proved to be sensitive to evaluate the soil quality, however further research is required to better understand the sustainability of shifting cultivation by including more physical, biological and chemical indicators. 相似文献
3.
The study of floral diversity in forest and its development are incomplete without taking consideration of plant-soil interactions. So with this view in mind, the present study was conducted in tropical semi-evergreen forests of the Mokukchung district, Nagaland, in eastern Himalaya. The aim of the study was to investigate the phytosociological parameters in relation to soil properties.Seven sites were randomly selected to study the soil properties at up to one meter in depth and a phytosociological study was carried out in nearby areas via the quadrate method. In the studied sites, the richness of tree species varied from 4 to 15 ha~(-1), with Gmelina arborea and Duabanga grandifloras being the common species.The highest total basal area was recorded in 10 mile village(47,998.16 cm~2ha~-1)) followed by Minkong village site(32,704.66 cm~2ha~(-1)). Soil physical and chemical properties—i.e. bulk density(BD), soil p H, organic carbon(OC),available nitrogen(N), available phosphorus(P), and available potassium(K) were analyzed using standard procedures. Significant differences were observed in the soil properties. The basal area of species showed significant positive correlation in terms of available K(0.754) and OM(0.302) content in soil, and the Shannon–Wiener diversity index(H) is also positively correlated with the available N content(0.402). The undisturbed nature of the sites played an important role in maintaining the soil fertility and floral diversity of the sites. Moreover, sites with maximum productivity and soil fertility are considered as potential carbon sequestration areas in the region while sites with the low soil fertility need restoration. 相似文献
4.
Reed James Kusters Koen Barlow Jos Balinga Michael Borah Joli Rumi Carmenta Rachel Chervier Colas Djoudi Houria Gumbo Davison Laumonier Yves Moombe Kaala B. Yuliani Elizabeth L. Sunderland Terry 《Landscape Ecology》2021,36(8):2395-2407
Landscape Ecology - Integrated landscape approaches (ILAs) that aim to balance conservation and development targets are increasingly promoted through science, policy, and the donor community.... 相似文献
5.
Genetic Parameters,Correlation and Selection Response for Submergence Tolerance in Rice 总被引：2，自引：0，他引：2
在每年经常遭受洪涝灾害的农田里,对一套水稻高代品系材料进行了耐淹性选择效果的研究表明,在受洪水影响的真实环境中所进行的选择是有效的;叶片出水后１０ｄ调查的植株成活率与株高呈显著的正相关;植株成活率的遗传力估值较高,而子粒产量的遗传力估值较低,因而在农田洪涝胁迫环境下基于耐淹性（或成活率）的选择较在相同环境下针对子粒产量的选择更有效。 相似文献
6.
Ashok Kumar R.K. Avasthe K. Rameash Brijesh Pandey Tasvina R. Borah Rinchen Denzongpa H. Rahman 《Scientia Horticulturae》2011
Field experiments were conducted during 2005–09 at ICAR Sikkim Centre, Tadong, East Sikkim, India at an altitude of 1400 m amsl to identify the suitable environment for high production of good quality fruits with less diseases for strawberry varieties Ofra and Chandler. Both the varieties were grown under low cost polyhouse, plastic tunnel and open conditions. Maximum number of flower trusses per plant was recorded in Chandler under open condition (13.0) followed by plastic tunnel (12.7). The maximum number of fruits per inflorescence was found in Ofra (7.12) under polyhouse while maximum number of runners per plant was observed in Ofra (12.3) in open conditions. Plastic tunnel with Ofra produced highest fruit weight (26.2 g), fruit length (5.5 cm) and fruit diameter (3.9 cm). Best fruit quality in terms of TSS (6.8%), lower acidity (0.83%) and total sugar (6.3%) was observed in Chandler under plastic tunnel conditions. The highest total fruit yield was recorded with Ofra under plastic tunnel (40.2 t/ha) but the maximum marketable yield was obtained in Chandler under plastic tunnel (35.3 t/ha). Diseases were found to be less prevalent in tunnel as compared to polyhouse and open conditions. 相似文献
7.
P. K. Pathak R. Roychoudhury J. Saharia M. C. Borah D. J. Dutta R. Bhuyan D. Kalita 《Tropical animal health and production》2018,50(5):1025-1032
The present study was formulated to find out the status of important season related thermal stress biomarkers of pure-bred (Hampshire) and crossbred (50% Hampshire × 50% local) pigs under the agro-climatic condition of Assam State, India. The experiment was also aimed to study the role of different level of energy ration (110, 100, and 90% energy of NRC feeding standard for pig) in variation of physiological and biochemical parameters in two genetic groups of pigs in different seasons. The metabolizable energy value were 3260, 2936.5, and 3585.8 kcal/kg in grower ration and 3260.2, 2936.6, and 3587 kcal/kg in finisher ration for normal energy (NE), low energy (LE) and high energy (HE), respectively. Both the genetic group of animals were housed separately under intensive system of management. Each pen was measuring 10′ × 12′ along with an outer enclosure. Six weaned piglets (almost similar body weight of average 10.55 kg) of each group were kept in a separate pen. However, after attainment of 35 kg body weight, the animals of a group were divided in two pens of three animals each. The present experiment indicated that average ambient temperature during summer months (27.33–29.51 °C) was above the comfort zone for pigs (22 °C). The significantly (P?<?0.01) higher relative humidity (RH) (%) was recorded in outdoor environment (87.26–91.10%) and in the morning time (86.60–91.10%). The temperature humidity index (THI) during the study period was found to be indicative of thermal stress to the experimental animals during summer (79.55–82.56). Physiological parameters viz., respiration rate (RR) and rectal temperature (RT) were significantly (P?<?0.01) higher in summer season (43.75–72.12 breaths/min. and 102.29–103.23 °F) and non-significantly higher values were recorded in Hampshire pigs. It was also found that the significantly (P?<?0.01) lower RR as well as RT was recorded in the pigs fed with high energy (HE) ration during summer season. Serum triiodothyronine (T3) and thyroxine (T4) concentrations were significantly (P?<?0.01) lower during summer, while both the genetic groups showed significantly (P?<?0.01) higher concentration of serum cortisol during summer season. It was also observed that thyroid hormone and cortisol concentrations were maintained in groups of pig fed vegetable oil incorporated HE diet during summer. From the present study, it is found that the increasing the energy level of the ration might be helpful to minimize the effects of thermal stress during summer. 相似文献
8.
We recorded gregarious flowering of three bamboo species viz. Bambusa arundinacea, Bambusa tulda and Melocanna baccifera during rainy season from early March to late May, 2008 at lowland forest of Bajali area of Indo-Burma hotspot region. A regular
method of field sampling of various forest types was used to map the bamboo species in Assam, India. Moreover, people’s perception
on bamboo blooming was investigated at 17 sample plots covering 258 respondents inhabiting at the site at an average of 15
in each plots. A floral clump (inflorescence) emerged and developed into a giant panicle comprising of numerous florets. Two
types of seeds viz. bacca and caryopsis were recorded in different species. Viviparous germination was noticed in few bacca
seeds produced by M. baccifera and this has unusual occurrence in bamboo. Seed viability test shows that 75% caryopsis seeds were viable; these seeds had
the potential of germination and can be used for mass regeneration. Most local respondents believe that bamboo flowering can
result in population explosion of rodent, therefore, they think bamboo flowering is positively correlated with famine. This
traditional belief results in massive destruction of bamboo clumps, which significantly impacts on rural economy. 相似文献
9.
An attempt was made to determine plasma concentrations of, 13, 14‐dihydro‐15‐keto‐prostaglandin F2α (PGFM), cortisol and progesterone during periparturient period in yak. Plasma PGFM level showed an increasing trend beginning day 5 prior to parturition (0.48 ± 0.14 ng/ml) and increased steeply thereafter to reach a peak level (17.16 ± 1.31 ng/ml) on the day of parturition. The levels, then, declined sharply on day 1 postpartum to reach 1.20 ± 0.40 ng/ml and thereafter declined gradually over the days to reach 0.28 ± 0.09 ng/ml on day 20 postpartum and this level was maintained with fluctuation within narrow limits thereafter till conclusion of the blood sampling on day 90 post‐calving. The plasma progesterone concentration on days 7 and 6 before parturition was high (2.10 ± 0.10 and 2.12 ± 0.10 ng/ml, respectively). The level then decreased gradually and abrupt fall was observed 1–2 days before parturition and became basal on day of parturition (0.24 ± 0.04 ng/ml). This basal level was maintained till the end of the blood sampling on day 90 postpartum. Plasma cortisol level showed an increasing trend beginning day 2 prior to parturition (2.36 ± 0.65 ng/ml) and increased steeply thereafter to reach a peak level (26.65 ± 5.28 ng/ml) on the day of parturition. The levels, then, declined gradually over the days and touched 2.36 ± 0.25 ng/ml on day 3 postpartum and this level was maintained with fluctuation within narrow limits thereafter till day 7 post‐calving. 相似文献
10.
An attempt was undertaken to investigate the efficacy of Ovsynch protocol for timed artificial insemination (TAI) with or without Norprolac (antiprolactin) treatment during non‐breeding season (winter months) in yaks (n = 25). During non‐breeding season, plasma prolactin profile has been reported high due to cold and nutritional stress. The Norprolac dose of i.m. administration was standardized for prolactin suppression. Three different doses viz. 2.5, 5.0 and 7.5 mg were attempted and the dose of 7.5 mg Norprolac i.m. per animal was found to be suitable for suppression of prolactin levels up to 30 h. Ovsynch treatment with Norprolac induced more number of oestrous symptoms per animal (4.8 vs 2.1), higher LH peak concentration (24.01 vs 16.16 ng/ml), longer duration of LH surge (6.8 vs 5.2 h) and higher conception rate (70 vs 30%) in Ovsynch plus Norprolac treated animals compared with animals treated with Ovsynch alone. Therefore, this study clearly indicates the opportunity for practical application of the Ovsynch plus Norprolac protocol for TAI in yaks during non‐breeding seasons. 相似文献
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