In vitro anti‐LPS dose determination of ketorolac tromethamine and in vivo safety of repeated dosing in healthy horses          下载免费PDF全文

A. W. Bianco  G. E. Moore  B. R. Cooper  S. D. Taylor 《Journal of veterinary pharmacology and therapeutics》2018,41(1):98-104

Flunixin meglumine (FM) is a commonly used Nonsteroidal anti‐inflammatory drug (NSAID) in horses, but clinical efficacy is often unsatisfactory. Ketorolac tromethamine (KT) demonstrates superior efficacy compared to other NSAIDs in humans, but its anti‐inflammatory effects have not been investigated in the horse. Safety of repeated dosing of KT has not been evaluated. The first objective was to conduct a dose determination study to verify that a previously described dosage of KT would inhibit Lipopolysaccharide (LPS)‐induced eicosanoid production in vitro, and to compare KT effects of this inhibition to those of FM. Then, a randomized crossover study was performed using nine healthy horses to evaluate plasma concentrations of KT and FM following IV administration. Administered dosages of KT and FM were 0.5 mg/kg and 1.1 mg/kg, respectively. Safety following six repeated doses of KT was assessed. Ketorolac tromethamine and FM suppressed LPS‐induced Thromboxane B₂ (TXB₂) and Prostaglandin E₂ (PGE₂) production in vitro for up to 12 hr. Intravenous administration produced plasma concentrations of KT and FM similar to previous reports. No adverse effects were observed. A KT dosage of 0.5 mg/kg IV inhibited LPS‐induced eicosanoids in vitro, and repeated dosing for up to 3 days appears safe in healthy horses. Investigation of in vivo anti‐inflammatory and analgesic effects of KT is warranted.  相似文献   

Treatment of severe immune-mediated thrombocytopenia with human IV immunoglobulin in 5 dogs     

Bianco D  Armstrong PJ  Washabau RJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(4):694-699

BACKGROUND: Glucocorticoids with or without other immunotherapy are the initial treatment of choice for dogs with severe immune-mediated thrombocytopenia (IMT). The majority of treated dogs will have improvements in platelet counts within 5 to 7 days of starting therapy, but complications from hemorrhage often occur before a response is seen. Human IV immunoglobulin (hIVIG) blocks Fc receptors on mononuclear phagocytic cells in dogs; it is used in people with idiopathic thrombocytopenic purpura. HYPOTHESIS: The purpose of this study was to describe adverse effects and benefit of hIVIG in addition to conventional immunosuppressive therapy in dogs with severe IMT. ANIMALS: Five client-owned dogs with severe primary IMT. METHODS: Case series. The hospital database was searched for dogs with primary IMT treated with hIVIG. RESULTS: No adverse effects were noted during or after hIVIG infusion in any treated dog. Over a 6-month follow-up, all dogs were clinically normal when using conventional immunosuppressive therapy. Human IVIG was administered 3 days after initiation of immunosuppressive therapy in 4 dogs, and, after 2 days, in 1 dog. In all dogs, the mean platelet counts pre- and 24 hours post-hIVIG infusion (0.28-0.76 g/kg) were 2,500/pL and 50,600/microL (62,750/microL for the 4 responders), respectively. One dog failed to respond as promptly to hIVIG (0.34 g/kg), and the platelet count increased to 66,000/microL after 9 days of immunosuppressive therapy. The mean duration of hospitalization post-hIVIG in all 5 dogs was 1.8 days (12 hours for responders), and the mean total length of hospitalization was 4.6 days (3.5 days for responders). Active hemorrhage resolved and no packed red blood cell transfusions were required after hIVIG infusion for responders. CONCLUSIONS AND CLINICAL IMPORTANCE: Human IVIG was well tolerated and appeared to be associated with rapid platelet count recovery and amelioration of clinical signs in most dogs with IMT.  相似文献   

Abstracts of papers presented at the 9th meeting of the international council for the study of viruses and virus diseases of the grapevine     

William B. Hewitt  Marc Fuchs  Bernard Walter  Monique Pinck  Lothaire Pinck  G. Belli  P. A. Bianco  A. Fortusini  Giovanni Granata  Anna Appiano  et al. 《Phytoparasitica》1989,17(1):57-78

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Use of enoxaparin in dogs with primary immune‐mediated hemolytic anemia: 21 cases          下载免费PDF全文

Cynthia M. Panek DVM  Reid K. Nakamura DVM  DACVECC  Domenico Bianco DVM  PhD  DACVIM 《Journal of Veterinary Emergency and Critical Care》2015,25(2):273-277

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Influence of phosphorus fertilization on the response of pinus genotypes to glyphosate subdoses     

de Andrade  Thiago Cavalcante Gomes Ribeiro  Bacha  Allan Lopes  de Camargo  Mariane Bueno  de Carvalho  Leonardo Bianco 《New Forests》2022,53(1):143-160

New Forests - Previous studies have reported that phosphorus transporters are involved in glyphosate uptake by plants, so phosphorus fertilization might act as an attenuator of glyphosate effects...  相似文献   

Transient Fanconi syndrome in Quarter horses     

Cameon M. Ohmes  Elizabeth G. Davis  Laurie A. Beard  Karie A. Vander Werf  Alex W. Bianco  Urs Giger 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(2):147-151

Two Quarter horses with weight loss had glucosuria, euglycemia, and a mild metabolic acidosis suggesting a proximal renal tubular defect. Further testing revealed transient generalized aminoaciduria, lactic aciduria, and glucosuria, indicating Fanconi syndrome. Both horses recovered with supportive therapy. This is the first report of acquired Fanconi syndrome in horses.  相似文献   

A retrospective evaluation of doxorubicin‐based chemotherapy for dogs with right atrial masses and pericardial effusion     

S. Ghaffari  D. C. Pelio  A. J. Lange  J. W. Arndt  J. D. Chretin  S. C. Fiocchi  D. Bianco  R. K. Nakamura 《The Journal of small animal practice》2014,55(5):254-257

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Space–time patterns of co‐variation of biodiversity and primary production in phytoplankton guilds of coastal marine environments     

Maria Rosaria Vadrucci  Fabio Vignes  Annita Fiocca  Alberto Basset  Immacolata Santarpia  Gian Carlo Carrada  Marina Cabrini  Serena Fonda Umani 《水产资源保护:海洋与淡水生态系统》2003,13(6):489-506

• 1. The relevance of biodiversity to ecosystem processes is a major topic in ecology. Here, we analyse the relationship between biodiversity and productivity of the nano‐ and micro‐phytoplankton guilds in coastal marine ecosystems.
• 2. The patterns of variation of species richness, diversity and primary productivity (as ¹⁴C assimilation) were studied in two marine areas: a eutrophic–mesotrophic area beside the River Po delta (northern Adriatic) and an oligotrophic area around the Salento peninsula (southern Adriatic–Ionian). The study was carried out at 23 sites in the northern area and at 45 sites in the southern area. Sites were arranged on expected spatial and temporal gradients of primary productivity variation, according to distance from the coast, optical depths and seasonal period.
• 3. 167 taxa were identified in the northern area and 153 taxa in the southern area. In both areas, the taxonomic composition of the nano‐ and micro‐phytoplankton guilds exhibited greater temporal than spatial variation. The latter was much higher in the southern area than in the northern area (average dissimilarity between stations being 70.7±0.8% and 44.7±4.2% respectively).
• 4. Primary productivity varied in space and time on the gradients considered. Phytoplankton species richness and diversity exhibited significant patterns of variation in space and time; overall, these were inversely related to the primary productivity patterns in the northern area, whereas they were directly related in the southern area.
• 5. The small individual size and the high turnover rate of phytoplankton are likely to underlie the observed relationships, which emphasized a threshold response to nutrient enrichment in agreement with the ‘paradox of enrichment’. Under resource enrichment conditions, the high turnover of producers leads to hierarchical partitioning of the available resources with an increasing dominance of a few species. Therefore, the relationship observed here seems likely to be explained by the complementarity hypothesis.

Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

Theta-bearing and complement-receptor lymphocytes are distinct populations of cells   总被引：27，自引：0，他引：27  

C Bianco  V Nussenzweig 《Science (New York, N.Y.)》1971,173(992):154-156

In the mouse, most (or all) complement-receptor lymphocytes and theta-bearing lymphocytes are part of nonoverlapping populations of cells. This finding validates the use of these membrane markers to characterize populations of lymphocytes. In addition, it is probable that, although receptors for complement and membrane-bound immunoglobulins coexist on the same lymphoid cells, these markers are located at separate sites on the membrane.  相似文献   

High molecular weight glutenin subunits in some durum wheat cultivars investigated by means of mass spectrometric techniques     

Muccilli V  Lo Bianco M  Cunsolo V  Saletti R  Gallo G  Foti S 《Journal of agricultural and food chemistry》2011,59(22):12226-12237

The primary structures of high molecular weight glutenin subunits (HMW-GS) of 5 Triticum durum Desf. cultivars (Simeto, Svevo, Duilio, Bronte, and Sant'Agata), largely cultivated in the south of Italy, and of 13 populations of the old spring Sicilian durum wheat landrace Timilia (Triticum durum Desf.) (accession nos. 1, 2, 3, 4, 7, 8, 9, 13, 14, 15, SG1, SG2, and SG3) were investigated using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and reversed-phase high performance liquid chromatography/nanoelectrospray ionization mass spectrometry (RP-HPLC/nESI-MS/MS). M(r) of the intact proteins determined by MALDI mass spectrometry showed that all the 13 populations of Timilia contained the same two HMW-GS with 75.2 kDa and 86.4 kDa, whereas the other durum wheat cultivars showed the presence of the expected HMW-GS 1By8 and 1Bx7 at 75.1 kDa and 83.1 kDa, respectively. By MALDI mass spectrometry of the tryptic digestion peptides of the isolated HMW-GS of Timilia, the 1Bx and 1By subunits were identified as the NCBInr Acc. No AAQ93629, and AAQ93633, respectively. Sequence verification for HMW-GS 1Bx and 1By both in Simeto and Timilia was obtained by MALDI mass mapping and HPLC/nESI-MSMS of the tryptic peptides. The Bx subunit of Timila presents a sequence similarity of 96% with respect to Simeto, with differences in the insertion of 3 peptides of 5, 9, and 15 amino acids, for a total insertion of 29 amino acids and 25 amino acid substitutions. These differences in the amino acidic sequence account for the determined Δm of 3294 Da between the M(r) of the 1Bx subunits in Timilia and Simeto. Sequence alignment between the two By subunits shows 10 amino acid substitutions and is consistent with the Δm of 148 Da found in the MALDI mass spectra of the intact subunits.  相似文献