Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey     

Gülşen Sertkaya  Marta Martini  Paolo Ermacora  Rita Musetti  Ruggero Osler 《Phytoparasitica》2005,33(4):380-390

During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases. Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY phytoplasma infecting Japanese plum was previously reported. http://www.phytoparasitica.org posting July 21, 2005.  相似文献   

Sanitary Monitoring Models for Wild Ungulate Stock Farms in Tuscany     

A. Martini  P. Marconi  M. P. Ponzetta  A. Giorgetti  M. Viliani 《Veterinary research communications》2005,29(2):77-82

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Extra-articular absorbable suture stabilization of coxofemoral luxation in dogs     

Martini FM  Simonazzi B  Del Bue M 《Veterinary surgery : VS》2001,30(5):468-475

OBJECTIVE: To evaluate the effectiveness of an extra-articular surgical technique using absorbable suture material for the stabilization of traumatic coxofemoral luxation in dogs. STUDY DESIGN: Prospective, clinical study. ANIMALS: Fourteen client-owned dogs with recent and long-standing traumatic coxofemoral luxation (13 craniodorsal and 1 ventral). METHODS: Coxofemoral luxations were surgically reduced and maintained in place with an extra-articular iliofemoral multifilamentous absorbable suture (3 to 6 strands of 2 USP Polyglactin 910). No external support was employed, and all the dogs were encouraged to use the affected limb postoperatively. The average time of clinical and radiographic follow-up was 11.6 +/- 6.3 months (from 2 to 22 months). RESULTS: During the follow-up period, no reluxations occurred and no complications associated with the surgical technique were identified. The dogs started bearing weight from 1 to 10 days after the surgery (mean, 4.3 +/- 2.9 days) and the period of lameness ranged from 7 to 30 days (20 +/- 8.6 days). At the final clinical examination, the dogs did not demonstrate any lameness or pain during passive flexo-extension movements, and there was no significant limitation of the range of motion. CONCLUSION: Extra-articular stabilization with multifilamentous absorbable sutures is a simple, effective method of treatment for acute and chronic coxofemoral luxation. The absorbable material used is strong enough to maintain articular stability during the period of scar tissue formation even in large-breed dogs. CLINICAL RELEVANCE: Absorbable sutures avoid the possible complications related to the use of nonabsorbable material and seem to be sufficient to maintain articular stability during the capsular healing process.  相似文献   

Leek Proliferation: A New Phytoplasma Disease in the Czech Republic and Italy     

A. Bertaccini  J. Fránová  S. Paltrinieri  M. Martini  M. Navrátil  C. Lugaresi  J. Nebesárová  M. Simkova 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(5):487-493

During the summer 1996, twelve of twenty-eight leek plants located in a garden near eské Budjovice, South Bohemia exhibited symptoms typical of diseases associated with phytoplasmas. In summer 1998 similar symptoms were detected in leek plants in a field used for seed production located in Romagna, North Italy. In both cases the plants were established in the spring of the previous year. Plants showed flower abnormalities: stamen elongation, anther sterility, pistil proliferation, as well as poor, if any, seed production. Phytoplasma-like structures were detected by scanning and transmission electron microscopy in phloem sieve elements in the Czech diseased plants, but not in healthy ones. Nested-PCR amplifications of extracted DNA with phytoplasma-specific oligonucleotide primer pairs confirmed the presence of phytoplasmas in these plants at low concentrations. Restriction fragment length polymorphism analyses of amplified ribosomal sequences allowed the identification of detected phytoplasmas: all the samples from the Czech Republic contained aster yellows related phytoplasmas (16SrI-B) while in the Italian samples aster yellows related phytoplasmas (16SrI-B) together with stolbur related phytoplasmas (16SrXII-A) were identified. This is the first report of detection and identification of a phytoplasma disease of leek in the Czech Republic and Italy.  相似文献   

Wert mathematischer Formulierungen     

E. Martini 《Journal of pest science》1932,8(1):10-11

Ohne Zusammenfassung  相似文献   

Entomologie und Hauptstadt     

E. Martini 《Journal of pest science》1939,15(4):43-46

Ohne Zusammenfassung Der Aufsatz ist meine Materialsammlung für die kurze Würdigung der Verdienste der Stadt Berlin um die Entomologie, mit der ich am 18. August 1938 im Rathaussaal der Stadt Berlin und ihrem Vertreter, Herrn Stadtrat Engel, für den Empfang des 7. Internationalen Entomologenkongresses gedankt habe.  相似文献   

Flow‐cytometric detection of phenotypic aberrancies in canine small clear cell lymphoma          下载免费PDF全文

V. Martini  A. Poggi  F. Riondato  M. E. Gelain  L. Aresu  S. Comazzi 《Veterinary and comparative oncology》2015,13(3):281-287

Histopathology and immunohistochemistry are mandatory to solve the differential between canine low‐grade lymphoma and reactive hyperplasia. However, clinicians and owners often show reluctance toward these invasive tests. However, molecular biology techniques are still not sensitive and specific enough to be regarded as a reliable tool for final diagnosis. In humans, flow cytometry (FC) allows a definitive diagnosis of T‐cell lymphoma based on high prevalence of antigen aberrancies. We describe here the immunophenotype of 26 cases of suspect canine small‐clear cell lymphoma, determined by multi‐colour FC. All cases showed antigen aberrancies and therefore neoplasia was always confirmed. As a consequence, we argue that the combined use of cytology and FC allows solving the differential diagnosis between small clear cell lymphoma and non‐neoplastic reactive conditions when histopathology is not available. Further studies are needed to establish if any aberrancy can be considered indicative of specific histotypes.  相似文献   

Cholecystocutaneous fistula containing multiple gallstones in a dog     

Martina Fabbi  Antonella Volta  Fausto Quintavalla  Elena Zubin  Sabrina Manfredi  Filippo M. Martini  Luciana Mantovani  Mario Tribaudino  Giacomo Gnudi 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(12):1163-1166

A 7-year-old dog was presented with a history of an open lesion on the right thoracic wall, discharging honey-like fluid and small stones. Ultrasonography and computed tomographic fistulography identified a cholecystocutaneous fistula; cholecystectomy was curative. Veterinarians should consider this disease in patients with long-term discharging lesions on the right thoracic or abdominal wall.  相似文献   

Microbiological Quality Assessment of Watershed Associated with Animal-Based Agriculture in Santa Catarina, Brazil     

Gilbert C. Sigua  Julio Cesar Pascale Palhares  Jalusa Deon Kich  Magda Regina Mulinari  Rosemari Martini Mattei  Jaqueline Bianca Klein  Susana Muller  Gustavo Plieske 《Water, air, and soil pollution》2010,210(1-4):307-316

Environmental problems many times could evolve when manure-containing pathogens are distributed into an open environment with no effort made to reduce the content of pathogens or limit their movement in the environment. Wind, surface flow, and subsurface flow can all carry enough pathogens to receiving waters to exceed water quality standards. This study was conducted to assess the microbiological quality of water associated with animal-based agriculture in the sub-basin of Pinhal River located in the rural area of Concordia, Santa Catarina, Brazil. Six sampling points representing different agricultural land uses (LU1—dairy cattle; LU2—without animals; LU3—dairy + pigs + poultry + crops; LU4—pigs + poultry + crops; LU5—dairy + pigs + poultry + crops + human; and LU6—dairy+pigs+crops) along the Pinhal River sub-basin (north to south) were sampled biweekly from August 2006 to December 2008. Concentrations of fecal coliforms and Escherichia coli varied significantly (p?≤?0.05) with land use (LU), but there was no interaction effect of LU, season, and time. Water samples from the catchment area of LU1 had the highest concentration of fecal coliforms (4,479?±?597 CFU ml^?1) when compared with other catchment areas. Catchment area associated with LU2 (no animal) had the lowest concentrations of fecal coliforms (39.2?±?5.2 CFU ml^?1). With the exception of LU2 (control site), all the maximum concentrations of E. coli exceeded the single maximum allowable concentration for E. coli (100 CFU ml^?1). When LU1 was compared with other catchment areas (LU3, 50%; LU4, 67%; LU5, 58%; and LU6, 44%), it had the lowest counts (39%) of Salmonella sp. Our results suggest that spatial pattern of bacterial water quality is evident, which can be linked to the different land uses and associated practices (present or absent of animal activities). Therefore, varying responses associated with the different land uses would be critical in identifying the importance of different sources of bacteria in the catchment area and the mechanisms transferring them.  相似文献   

Modification of sunflower oil quality by seed-specific expression of a heterologous Δ9-stearoyl-(acyl carrier protein) desaturase gene     

P. Rousselin    J. Molinier    C. Himber    D. Schontz    P. Prieto-Dapena  J. Jordano    N. Martini    S. Weber    R. Horn    M. Ganssmann    R. Grison    M. Pagniez    A. Toppan    W. Friedt  G. Hahne 《Plant Breeding》2002,121(2):108-116

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