Detection of hemorrhagic septicemia virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two noncompetitive monoclonal antibodies against early viral nucleoproteins.     

F A Sanz  J M Coll 《American journal of veterinary research》1992,53(6):897-903

Inclusion of high-ionic strength buffers helped us to develop a sandwich ELISA to detect hemorrhagic septicemia virus (HSV) in cell culture and infected trout tissue extracts. For maximal sensitivity of 0.1 to 0.2 ng/well/100 microliters or about 10 to 50 TCID50/well/100 microliters, trout extracts were diluted 1:1 and assayed for the earliest synthesized nucleoprotein N. Simultaneous binding of the N protein from HSV in the sample to the wells coated with monoclonal antibody (2D5 against the N protein) and to the peroxidase-labeled monoclonal antibody (2C9 against the N protein) proceeded during a 2-hour incubation at 20 to 22 C (room temperature). The response was linear between 6 to 60 ng/well of purified virus. Monoclonal antibodies used were noncompetitive with each other and reacted with F1, F2, 23.75, and 5 Spanish isolates of HSV, but not with infectious hematopoietic necrosis or infectious pancreatic necrosis viruses. Tissue specimens with low content of HSV virus may now be assayed directly without use of cell culture, rapidly, and with high precision, during the acute phase of the disease in salmonid fishes.  相似文献   

A survey of northern Victorian dairy farmers to investigate dairy calf management: colostrum feeding and management          下载免费PDF全文

AJ Phipps  DS Beggs  AJ Murray  PD Mansell  MF Pyman 《Australian veterinary journal》2018,96(4):101-106

Objectives

To describe colostrum management practices carried out in northern Victorian dairy herds and to identify weaknesses in these areas that may affect calf health and welfare by comparing the results with the current industry recommendations

Methods

A questionnaire to obtain information about colostrum management and calf‐rearing practices was sent to commercial dairy farming clients of Rochester Veterinary Practice between June and September 2013. The questionnaire consisted of a general herd overview and colostrum harvesting practices.

Results

The response rate was 39% (58/150). Many dairy producers were not meeting the current industry recommendations in the following areas: (1) time of removal calf from the dam, (2) relying on calf suckling colostrum from the dam to achieve adequate passive transfer, (3) failing to supplement calves with colostrum, (4) feeding inadequate volumes of colostrum, (5) delayed colostrum harvesting, (6) pooling of colostrum, (7) failing to objectively assess colostrum quality or relying on visual assessment and (8) storing colostrum for a prolonged periods of time at ambient temperatures.

Conclusion

The results from this survey highlight the need for greater awareness of industry standards for colostrum management and feeding hygiene.

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BVDV in Australian alpacas: natural infection and clinical profiles following co‐mingling with a persistently infected heifer          下载免费PDF全文

CA Evans  E Erregger  F Hemmatzadeh  PD Cockcroft 《Australian veterinary journal》2018,96(7):262-268

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Seroprevalence of antibodies to Pestivirus infections in South Australian sheep flocks          下载免费PDF全文

CA Evans  SR Lanyon  RM O’Handley  MP Reichel  PD Cockcroft 《Australian veterinary journal》2018,96(8):312-314

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Acute‐phase proteins as diagnostic markers in horses with colic          下载免费PDF全文

Tina H. Pihl DVM  PhD  Elrien Scheepers BVSc  MSc  Macarena Sanz DVM  MS  DACVIM  Amelia Goddard BVSc  MMed Vet  Nils Toft Cand Sci  PhD  Mads Kjelgaard‐Hansen DVM  PhD  Pia H. Andersen DVM  PhD  DVSc  Stine Jacobsen DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2016,26(5):664-674

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Ostreid herpesvirus-1 microvariant surveillance in Pacific oysters (Magallana gigas,Thunberg, 1793) in Australia in 2011     

BJ Moloney  M Deveney  K Ellard  P Hick  PD Kirkland  NJG Moody  J Frances 《Australian veterinary journal》2023,101(9):345-355

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An outbreak of Akabane virus—induced abnormalities in calves after agistment in an endemic region     

S. JAGOE  PD KIRLAND†  PAW HARPER‡ 《Australian veterinary journal》1993,70(2):56-58

SUMMARY: During 1988, 2 farmers in the Bega district agisted pregnant cattle in the Hunter Valley of New South Wales. On return to the district to calve, 54% of calves from herd 1 and 30% of calves from herd 2 were affected with congenital arthrogryposls or hydranencephaly caused by Akabane virus infection. Field observations and laboratory findings from this outbreak are presented, illustrating the danger of moving immunologlcally naive animals into areas where Akabane virus is endemic.  相似文献   

Rotavirus and concurrent infections with other enteropathogens in neonatal diarrheic dairy calves in Spain     

García A  Ruiz-Santa-Quiteria JA  Orden JA  Cid D  Sanz R  Gómez-Bautista M  de la Fuente R 《Comparative immunology, microbiology and infectious diseases》2000,23(3):175-183

Faeces samples from 218, one to 30 days old, diarrheic dairy calves in 65 dairy herds were screened for the presence of rotavirus and concurrent infections with coronavirus, Cryptosporidium, F5+ Escherichia coli and Salmonella spp. Calves were grouped according to their age as follows: 1-7, 8-14, 15-21 and 22-30 days. Rotavirus infection was detected in 46.9%, 45.6%, 33.8% and 48.3% of the calves in the respective age-groups. No significant differences in the detection rate of rotavirus were found among calves on the different age-groups. Rotavirus was the only enteropathogen detected in 39 of the 93 (41.9%) diarrheic calves positive to this agent. Concurrent infections with other enteropathogen(s) were detected in 31.3%, 33.3%, 20.6% and 3.4% of the rotavirus infected calves in the age-groups 1-7, 8-14, 15-21 and 22-30 d, respectively. A significant age-associated decrease in the detection rate of mixed infections (p < 0.01) was found. The detection rates of the other enteropathogens considered in calves with rotavirus infection were 20.4% for coronavirus, 85.2% for Cryptosporidium, 16.7% for F5+ E. coli and 1.8% for Salmonella.  相似文献   

Otto Martin Radostits 1934 – 2006     

DC Blood  CC Gay  KW Hinchcliff  PD Constable 《Australian veterinary journal》2007,85(6):231-231

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Plasma atrial natriuretic peptide concentration in warmblood horses with heart valve regurgitations     

Heidrun Gehlen PD  Dr Med Vet  Dipl ECEIM  Till Sundermann Dr Med Vet  Karl Rohn Dr Med Vet  Peter Stadler Prof  Dr Med Vet 《Journal of Veterinary Cardiology》2007,9(2):99-101

ObjectivesThis study measured plasma atrial natriuretic peptide (ANP) concentration in horses with heart valve regurgitations (HVR) with and without atrial and ventricular dilatation.BackgroundIn humans and small animals, plasma ANP concentration is increased in heart disease and correlates with the severity of clinical signs and heart enlargement.Animals, materials and methodsTen healthy horses (control) and 36 horses with HVR were evaluated by auscultation, electrocardiography, echocardiography, and determination of plasma ANP.ResultsControl horses demonstrated mean plasma ANP concentration of 21 ± 5.4 pg/mL. Of the 36 horses with HVR, 17 horses possessed normal echocardiographic heart size (group 1), 10 horses had a left atrial dilatation (group 2) and 9 horses had both left atrial and ventricular dilatation (group 3). Mean plasma ANP concentration of groups 1, 2 and 3 was 20.1 ± 5.6 pg/mL, 22.9 ± 11.0 pg/mL and 27.6 ± 17.4 pg/mL, respectively. The plasma ANP concentrations of HVR and control horses were not significantly different. The highest ANP concentrations were observed in horses with atrial and ventricular dilatation. No correlation between left atrial or ventricular size, weight, or sex and the plasma ANP concentration was found.ConclusionsNo significant differences in plasma ANP concentration was observed between groups. Further study, especially in horses with clinical signs of heart failure is needed.  相似文献