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1.
To improve soil fertility, efforts need to be made to increase soil organic matter content. Conventional farming practice generally leads to a reduction of soil organic matter. This study compared inorganic and organic fertilisers in a crop rotation system over two cultivation cycles: first crop broad bean (Vicia faba L.) and second crop mixed cropped melon-water melon (Cucumis melo-Citrullus vulgaris) under semi-arid conditions. Total organic carbon (TOC), Kjeldahl-N, available-P, microbial biomass C (Cmic), and N (Nmic), soil respiration and enzymatic activities (protease, urease, and alkaline phosphatase) were determined in soils between the fourth and sixth year of management comparison. The metabolic quotient (qCO2), the Cmic/Nmic ratio, and the Cmic/TOC ratio were also calculated. Organic management resulted in significant increases in TOC and Kjeldahl-N, available-P, soil respiration, microbial biomass, and enzymatic activities compared with those found under conventional management. Crop yield was greater from organic than conventional fertilizer. The qCO2 showed a progressive increase for both treatments during the study, although qCO2 was greater with conventional than organic fertilizer. In both treatments, an increase in the Cmic/Nmic ratio from first to second crop cycle was observed, indicating a change in the microbial populations. Biochemical properties were positively correlated (p < 0.01) with TOC and nutrient content. These results indicated that organic management positively affected soil organic matter content, thus improving soil quality and productivity.  相似文献   
2.
Mediterranean ecosystems are characterized by large arid areas where the patchy distribution of trees offers little protection against harsh climate conditions for seedling establishment. Climate change is predicted to result in an increase in these arid regions, with pronounced effects on vegetation. Production of seedlings with developed ectomycorrhizas is a promising strategy for minimizing the initial transplant shock, thereby increasing plant survival and growth during the first, most critical years of a plantation. One important species in the Mediterranean basin is Quercus suber (cork oak), which occurs, together with other evergreen oak species, in an agro-silvo-pastoral system that represents an example of sustainable land use in Europe. In this study, a Pisolithus tinctorius isolate was used for ectomycorrhizal colonization of cork oak nursery seedlings, and the effects on aboveground plant growth and leaf structural and physiological parameters were investigated. Ectomycorrhizal development resulted in a significant increase in leaf area, dry weight, nitrogen content, and photosynthetic pigments, and mycorrhizal plants showed a higher photosynthetic capacity and water use efficiency. Nursery-inoculated plants established in the field showed increased survival and growth during the first year after transplant. These results indicate a potential for further enhancing the use of mycorrhizal inoculation as a cultivation practice in forest nurseries. Considering the difficulty of soil restoration under limiting environmental conditions, nursery inoculation with ectomycorrhizal fungi can be an important advantage for improving the quality of seedling stock and its performance after out-planting in the field, benefiting the regeneration of arid regions and the reintroduction of inocula of ectomycorrhizal fungi into these areas.  相似文献   
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4.
Short- and long-term field experiments are necessary to provide important information about how soil carbon sequestration is affected by soil tillage system; such systems can also be useful for developing sustainable crop production systems. In this study, we evaluated the short- and long-term effects of conservation tillage (CT) on soil organic carbon fractions and biological properties in a sandy clay loam soil. Both trials consisted of rainfed crop rotation systems (cereal–sunflower–legumes) located in semi-arid SW Spain. In both trials, results were compared to those obtained using traditional tillage (TT). Soil samples were taken in flowering and after harvesting of a pea crop and collected at three depths (0–5, 5–10 and 10–20 cm). The soil organic carbon fractions were measured by the determination of total organic carbon (TOC), active carbon (AC) and water soluble carbon (WSC). Biological status was evaluated by the measurement of soil microbial biomass carbon (MBC) and enzymatic activities [dehydrogenase activity (DHA), o-diphenol oxidase activity (DphOx), and β-glucosidase activity (β-glu)].The contents of AC and MBC in the long-term trial and contents of AC in the short-term trial were higher for CT than TT at 0–5 cm depth for both sampling periods. Furthermore, DHA and β-glucosidase values in the July sampling were higher in the topsoil under conservation management in both trials (short- and long-term). The parameters studied tended to decrease as depth increased for both tillage system (TT and CT) and in both trials with the exception of the DphOx values, which tended to be higher at deeper layers.Values of DHA and β-glu presented high correlation coefficients (r from 0.338 to 0.751, p ≤ 0.01) with AC, WSC and TOC values in the long-term trial. However, there was no correlation between either TOC or MBC and the other parameters in the short-term trial. In general, only stratification ratios of AC were higher in CT than in TT in both trials. The results of this study showed that AC content was the most sensitive and reliable indicator for assessing the impact of different soil management on soil quality in the two experiments (short- and long-term).Conservation management in dryland farming systems improved the quality of soil under our conditions, especially at the surface layers, by enhancing its storage of organic matter and its biological properties, mainly to long-term.  相似文献   
5.
Veiled chameleons (Chamaeleo calyptratus) and panther chameleons (Furcifer pardalis) are the most popular chameleons over the world, and consequently, two of the most frequent species presenting to veterinary practices. However, published studies on normal ultrasonographic anatomy for these lizards are currently lacking. The objectives of this prospective anatomic study were to develop an ultrasound protocol for evaluation of the coelomic cavity in these species and describe the normal ultrasonographic anatomy of the coelomic organs. Seventeen healthy veiled chameleons and 15 healthy panther chameleons were included. A linear 18 MHz transducer was used. Chameleons were sedated and restrained in right lateral recumbency by an assistant. Longitudinal and transverse images were acquired, and authors recorded qualitative and quantitative ultrasonographic characteristics of the coelomic structures. The kidneys, liver, caudal vena cava, hepatic veins, portal vein, gallbladder, wall of the stomach and intestine, gonads and, when distended, urinary bladder could be visualized during ultrasonography of the coelomic cavity in both species. The spleen, pancreas, and adrenal glands could not be identified. Findings from the current study supported the use of ultrasonography for veiled chameleons (Chamaeleo calyptratus) and panther chameleons (Furcifer pardalis) with suspected intracoelomic diseases and provided normal reference information for future studies of these chameleon species.  相似文献   
6.
Multienoic fatty acids, such as linolenic acid, show their ability to interact with and to penetrate into model biomembranes by biomimetic experiments performed to support the absorption route followed by n-3 fatty acid in cells. The thermotropic behavior of model biomembranes, that is, dimyristoylphosphatidylcholine multilamellar or unilamellar vesicles, interacting with linolenic acid was investigated by differential scanning calorimetry. When dispersed in liposomes during their preparation, the examined biomolecule was found to interact with the phospholipid bilayers by modifying the gel to liquid-crystal phase transition of lipid vesicles; this modification is a function of the fatty acid concentration. Calorimetric analysis was also performed on samples obtained by leaving the pure n-3 acid in contact with lipid aqueous dispersions (multilamellar or unilamellar vesicles) and then examining the thermotropic behavior of these systems for increasing incubation times at temperatures higher than the transitional lipid temperature. Linolenic acid (LNA) was able to migrate through the aqueous medium and successively to interact with the vesicle surface and to penetrate into the model membranes, following a flip-flop mechanism, with a faster and higher effect for unilamellar vesicles, caused by the larger lipid surface exposed, compared to the multilamellar ones, although due to the lipophilic nature of LNA, such a transfer is hindered by the aqueous medium. The relevance of the medium in LNA absorption has been well clarified by other biomimetic transfer experiments, which showed the LNA transfer from loaded multilamellar vesicles to empty vesicles. Taken together, the present findings support the hypothesis of a passive n-3 acid transport as the main route of absorption into cell membranes.  相似文献   
7.
Tropical Animal Health and Production - This study aims at identifying mammary gland genes expressed in Brazilian Holstein cattle produced under tropical conditions, as compared to the Portuguese...  相似文献   
8.
Landscape Ecology - Urbanisation is an environmental filter for many species that leads to community homogenisation, with a few species inhabiting isolated patches (e.g. public and private gardens...  相似文献   
9.
Intramammary infections are a serious problem for dairy sheep farms, and Staphylococcus epidermidis is one of the main etiological agents of ovine mastitis. In this work, 131 S. epidermidis isolates, collected from 2201 dairy Sarda sheep belonging to 14 flocks with high somatic cell count scores, were studied. The flocks were located in diverse geographical areas of Sardinia, Italy. The aim of study was to assess the susceptibility of isolates to 13 antimicrobial agents, many of which are frequently used in mastitis therapy. Oxacillin was used for detecting methicillin-resistant S. epidermidis (MRSE) by disk diffusion test. Thirty-eight percent of the isolates (n = 50) were resistant to penicillin, 7.6% (n = 10) were resistant to tetracycline, and 2.3% (n = 3) were resistant to both penicillin and tetracycline (PTRSE). Two isolates were resistant to five antimicrobials including methicillin. Analysis of staphylococcal cassette chromosome mec (SCCmec) elements showed that both MRSE isolates harbored SCCmec type IVa. Based on pulsed-field gel electrophoresis (PFGE) typing by SmaI macrorestriction, S. epidermidis isolates were grouped into four clusters at 75% similarity level. The two multi-drug resistant MRSE isolates displayed distinct PFGE patters. This study indicates that S. epidermidis isolates from sheep milk samples may accumulate resistance markers for different antimicrobial agents. Furthermore, the occurrence of PTRSE and MRSE suggests to adopt adequate hygienic measures when handling animals with intramammary infections, in order to prevent spreading PTRSE and MRSE strains to humans through direct contact and/or consumption of contaminated food.  相似文献   
10.
European Journal of Plant Pathology - Plants are sessile organisms being constantly under a wide array of environmental pressures. Their resistance against biotic stress is regulated by...  相似文献   
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