苹果属无融合生殖SERKs基因家族的克隆和表达分析     

张丽杰  郭苏漫  董文轩 《分子植物育种》2015,(5)

本研究利用同源克隆法以苹果属平邑甜茶和杂种后代叶片为试验材料,克隆得到4个SERK基因家族片段,并分析了SERKs家族基因在平邑甜茶和杂种后代不同器官和组织中的表达情况。研究结果表明:分离获得的4个SERK基因家族片段的氨基酸序列与模式植物拟南芥、烟草等植物的同源性均在84%以上,同时与其他物种的氨基酸序列进行Blast比对都具有较高的同源性;RT-PCR定量分析结果表明SERK1、SERK2、SERK3、SERK4基因主要在生殖器官中表达。因此,推测SERKs基因在平邑甜茶和杂种后代生殖发育过程中起着重要的调控作用。本研究结果为进一步揭示无融合生殖的发生机理奠定了基础。  相似文献   

Composts Vary in Their Effect on Soil P Pools and P Uptake by Wheat     

Suman Lata Verma  Chris Penfold 《Communications in Soil Science and Plant Analysis》2017,48(4):459-468

With diminishing world reserves of phosphorus (P) deposits and rising fertilizer prices, it is important to find alternate sources of P for crops. The aim of this research was to evaluate the effect of four different composts C1 (animal manure and straw), C2 (garden waste), C3 (wood chips and bark), and C4 (kitchen waste) on soil P pools and P uptake by wheat on 14, 28, and 72 days after compost application. The composts were applied as a 2.5 cm thick layer on the soil surface. During sampling, only the soil underlying compost was sampled. Soil pH and total organic carbon were not affected by the amendments. Soil respiration was significantly higher in compost-amended soils compared with the unamended soil except with C4 on day 72. Addition of composts increased plant growth, and P uptake being highest on day 72 with C1 and C4. With little effect on available P concentration on day 14, there was a conversion of organic P into inorganic P in the compost treatments suggesting net mineralization of organic P on day 28. On day 72, the concentrations of the less labile P forms were higher in the compost treatments compared with the unamended suggesting precipitation and fixation as well as synthesis of organic P. This study showed that mulching with composts having high available and total P concentrations can provide plants with P and also increase soil P concentrations which could reduce the fertilizer requirement for the following crop.  相似文献   

Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent     

Mwangi DM  McKeever DJ  Nyanjui JK  Barbet AF  Mahan SM 《Veterinary immunology and immunopathology》2002,85(1-2):23-32

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Prevalence and risk factors of Coxiella burnetii seropositivity in Danish beef and dairy cattle at slaughter adjusted for test uncertainty     

Suman Paul  Jens F. Agger  Jørgen S. Agerholm  Bo Markussen 《Preventive veterinary medicine》2014

Antibodies to Coxiella burnetii have been found in the Danish dairy cattle population with high levels of herd and within herd seroprevalences. However, the prevalence of antibodies to C. burnetii in Danish beef cattle remains unknown. The objectives of this study were to (1) estimate the prevalence and (2) identify risk factors associated with C. burnetii seropositivity in Danish beef and dairy cattle based on sampling at slaughter.  相似文献   

Hybridizing <Emphasis Type="Italic">Brassica rapa</Emphasis> with wild crucifers <Emphasis Type="Italic">Diplotaxis erucoides</Emphasis> and <Emphasis Type="Italic">Brassica maurorum</Emphasis>     

Harsh Garg  Shashi Banga  Payal Bansal  Chhaya Atri  S. S. Banga 《Euphytica》2007,156(3):417-424

Two wide hybrids, Diplotaxis erucoides (2n = 14) × Brassica rapa (2n = 20) and B. maurorum (2n = 20) × B. rapa, were developed using the sequential ovary–ovule culture. Reciprocal crosses failed, possibly as a consequence of strong unilateral incompatibility. The F ₁ hybrids in each combination were completely male sterile and morphologically intermediate to the respective parents. DNA marker polymorphism and chromosome counts confirmed their hybrid nature. High frequency of bivalents in the F ₁ and the presence of trivalents/quadrivalents in the derived amphiploids suggested genomic duplications and homoeology of the parental genomes. Up to three homoeologous pairs between the D. erucoides (D^eD^e) and B. rapa (AA) genomes, and one between B. maurorum (B^mB^m) and B. rapa genomes were observed. Successful synthesis of the F ₁ hybrids and amphiploids of B. rapa with D. erucoides and B. maurorum, and allosyndetic chromosome pairing are expected to permit introgressions of desirable loci into the cultivated Brassica germplasm, especially for resistance to Alternaria brassicae and Albugo candida.  相似文献   

QTL analysis for phytonutrient compounds and the antioxidant molecule in mustard (Brassica juncea L.)     

Shilpa Gupta  M. K. Sangha  Gurpreet Kaur  Shashi Banga  Mehak Gupta  Hitesh Kumar  S. S. Banga 《Euphytica》2015,201(3):345-356

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Nodule-specific expression of Rhizobium meliloti symbiotic promoters P1 and P2 in chickpea-Rhizobium sp. symbiosis     

S. P. S. Khanuja  A. Suman  G. Singh  V. Kumar 《Biology and Fertility of Soils》1994,18(2):132-136

Developmentally specific expression of Rhizobium spp. genes involved in symbiotic N₂ fixation is known to operate through cascade regulation of various nif and fix operons. Fusion constructs of lacZ under symbiotic promoters P1 (for nifHDK operon) and P2 (for fixABCX operon) of Rhizobium meliloti were mobilized into Rhizobium spp. (Cicer) strains Rcd301 and RCR13. The assays for -galactosidase activity to monitor the expression of lacZ under these promoters was performed in host backgrounds of Escherichia coli, R. meliloti, and Rhizobium spp. (Cicer). The enzyme assays indicated significant levels of expression from P1 and P2 promoters in chickpea rhizobia, specifically in symbiotic cells from nodules. However, as in R. meliloti, these promoters did not induce strong expression in free-living cells of Rhizobium spp. (Cicer). This indicates functional homology of R. meliloti promoters in rhizobium spp. (Cicer). Functional cross-reactivity of trans regulatory factors like NtrA, NtrC, and NifA between these rhizobia seems evident from the nodule-specific expression of P1 and P2 cis elements.  相似文献   

Water use efficiency in a soybean field: influence of plant water stress     

Dennis D Baldocchi  Shashi B Verma  Norman J Rosenberg 《Agricultural and Forest Meteorology》1985,34(1):53-65

Measurements were made in 1980 over a fully-developed soybean (Glycine max (L.) Merrill) canopy at Mead, Nebraska to determine how crop water status influences photosynthesis, evapotranspiration and water use efficiency. Water use efficiency was calculated in terms of the CO₂—water flux ratio (CWFR). Micrometeorological techniques were used to measure the exchange rates of CO₂ and water vapor above the crop canopy. Crop water status was evaluated by reference to volumetric soil moisture (θ_v), stomatal resistance (r_s), and leaf water potential (ψ) measurements.Stomatal resistance (r_s) was independent of ψ when the latter was greater than ?1.1 MPa. r_s increased sharply as ψ dropped below this threshold. Canopy CO₂ exchange (F_c) decreased logarithmically with increasing r_s under strong irradiance. Although F_c was found to be strongly correlated with r_s, the influence of low values of ψ and of high air temperature cannot be discounted since these factors affect the enzymatic reactions associated with photosynthesis. Stomatal closure also reduced evapotranspiration and influenced the partitioning of net radiation.Under strong irradiance the CO₂ water flux ratio (CWFR) decreased with increasing stomatal resistance. This observation is at variance with predictions of certain early ‘resistance’ models, but substantiates predictions of some recent models in which leaf energy balance considerations are incorporated.  相似文献   

Characterization of bioactive recombinant woodchuck interleukin-2 amplified by RLM-RACE and produced in eukaryotic expression system     

Gujar SA  Michalak TI 《Veterinary immunology and immunopathology》2006,112(3-4):183-198

Woodchucks (Marmota monax) infected with woodchuck hepatitis virus (WHV) represent a highly valuable laboratory model of hepatitis B virus (HBV) infection, in which molecular, immunological and pathological events occurring in infected humans are adequately reflected. To advance studies on T cell immune responses and propagation of hepadnavirus in T lymphocytes in this animal model, we determined the complete sequence of woodchuck interleukin-2 (wIL-2) cDNA by utilizing RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) reaction. The wIL-2 sequence revealed a single open reading frame encoding for the predicted precursor protein comprised of a signal peptide and a 134 amino acid-long mature protein. The mature wIL-2 protein produced in the Escherichia coli expression system, designated as ec-rwIL-2, was found to be immunogenic but not biologically active. In contrast, precursor wIL-2 protein cloned into baculovirus transfer vector and expressed in Sf9 cells, designated as bac-rwIL-2, demonstrated functional competence. Further, bac-rwIL-2 was able to stimulate proliferation and to induce multiple daughter cell generations in woodchuck T cells, as well as facilitated the survival of standard IL-2-dependent mouse CTLL-2 cells in culture. Western blot analysis of bac-rwIL-2 using antibodies generated against ec-rwIL-2 revealed a single protein band of 15.5kDa. The availability of biologically active recombinant wIL-2 should facilitate ex vivo studies on functional competence of woodchuck T lymphocytes derived from different stages of hepadnaviral hepatitis and assist in recognizing their contribution to the pathogenesis of liver injury in the woodchuck model of hepatitis B.  相似文献   

Application of day and night digital photographs for estimating maize biophysical characteristics     

Toshihiro Sakamoto  Anatoly A. Gitelson  Brian D. Wardlow  Timothy J. Arkebauer  Shashi B. Verma  Andrew E. Suyker  Michio Shibayama 《Precision Agriculture》2012,13(3):285-301

In this study, an inexpensive camera-observation system called the Crop Phenology Recording System (CPRS), which consists of a standard digital color camera (RGB cam) and a modified near-infrared (NIR) digital camera (NIR cam), was applied to estimate green leaf area index (LAI), total LAI, green leaf biomass and total dry biomass of stalks and leaves of maize. The CPRS was installed for the 2009 growing season over a rainfed maize field at the University of Nebraska-Lincoln Agricultural Research and Development Center near Mead, NE, USA. The vegetation indices called Visible Atmospherically Resistant Index (VARI) and two green–red–blue (2g–r–b) were calculated from day-time RGB images taken by the standard commercially-available camera. The other vegetation index called Night-time Relative Brightness Index in NIR (NRBI_NIR) was calculated from night-time flash NIR images taken by the modified digital camera on which a NIR band-pass filter was attached. Sampling inspections were conducted to measure bio-physical parameters of maize in the same experimental field. The vegetation indices were compared with the biophysical parameters for a whole growing season. The VARI was found to accurately estimate green LAI (R² = 0.99) and green leaf biomass (R² = 0.98), as well as track seasonal changes in maize green vegetation fraction. The 2g–r–b was able to accurately estimate total LAI (R² = 0.97). The NRBI_NIR showed the highest accuracy in estimation of the total dry biomass weight of the stalks and leaves (R² = 0.99). The results show that the camera-observation system has potential for the remote assessment of maize biophysical parameters at low cost.  相似文献