Gender and the profession — an issue or not?          下载免费PDF全文

Darcy Shaw 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(1):12-13

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A ROLLER BOTTLE TISSUE CULTURE SYSTEM     

Shaw DT  Kingsland LC  Brues AM 《Science (New York, N.Y.)》1940,91(2354):148-149

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Seroprevalence of Ornithobacterium rhinotracheale infection in commercial laying hens in the north central region of the United States.     

C J Heeder  V C Lopes  K V Nagaraja  D P Shaw  D A Halvorson 《Avian diseases》2001,45(4):1064-1067

This study was the first to examine the seroprevalence of Ornithobacterium rhinotracheale (ORT) within a commercial egg layer population. Serum samples collected from egg production companies were examined by serum plate agglutination test (SPAT) and outer membrane protein-based enzyme-linked immunosorbent assay (ELISA). Results show that 90% of layer flocks were positive by SPAT and 100% by ELISA. Of the pullet flocks examined, 43% and 52% were positive by SPAT and ELISA, respectively. Our study indicates that the prevalence of ORT antibody is high in the commercial layer population, suggesting that this respiratory pathogen can easily spread through multiple-age layer farms from older flocks to newly housed pullet flocks.  相似文献   

A 31P topical magnetic resonance study of embryonic development in hens' eggs     

P. S. Belton  R. E. Gordon  J. M. Jones  D. Shaw 《British poultry science》1983,24(3):429-433

1. A ³¹P topical magnetic resonance study of whole chicken's eggs in vivo is described.

2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.

3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.

4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs.  相似文献   

Geographical cline of DNA variation within the F intersterility group of Heterobasidion annosum in Italy     

N. LA PORTA  P. CAPRETTI  K. KAMMIOVIRTA  R. KARJALAINEN  & K. KORHONEN 《Plant pathology》1997,46(5):773-784

Eighty-six Heterobasidion annosum isolates, mainly belonging to the F intersterility group and obtained from 32 different geographical localities in Italy, were subjected to genetic analysis by the Random Amplified Polymorphic DNA (RAPD) markers. The similarity between F and S groups was higher than that between F and P. In UPGMA Cluster Analysis, the F isolates originating from the same locality usually grouped in the same cluster. The isolates also showed a tendency to group at the level of larger geographical areas. Within the F group, isolates from the south of the Italian peninsula showed the highest genetic variation and northern isolates from the Alpine regions showed the lowest. This indicates a gradual cline along the peninsula. The genetic variability in the Italian F group is discussed in relation to the past and present distribution of the host species in Italy and Europe.  相似文献   

Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease          下载免费PDF全文

E. Milne  Y. Martinez Pereira  C. Muir  T. Scase  D. J. Shaw  G. McGregor  L. Oldroyd  E. Scurrell  M. Martin  C. Devine  H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271

Objectives

To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.

Materials and Methods

Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.

Results

Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.

Clinical Significance

An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted.  相似文献   

Laryngeal mask airway and transient hypercapnic hyperpnea for video‐endoscopic assessment of unilateral laryngeal paralysis in dogs          下载免费PDF全文

Daniel M. Sakai MV  DACVAA  Manuel Martin‐Flores MV  DACVAA  Amanda K. Jones PhD  Galina M. Hayes BVSc  PhD  DACVECC  DACVS‐SA  Marina J. McConkey DVM  DACVS‐SA  Jonathan Cheetham VetMB  PhD  DACVS‐LA 《Veterinary surgery : VS》2018,47(4):543-548

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Initial analytic quality assessment and method comparison of an immunoassay for adrenocorticotropic hormone measurement in equine samples          下载免费PDF全文

Katherine L. Irvine  Kay Burt  Andrew J. Hill  Stephanie Shaw  Kostas Papasouliotis 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(1):154-163

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Identification of infrared absorption spectral characteristics of synovial fluid of horses with osteochondrosis of the tarsocrural joint     

Vijarnsorn M  Riley CB  Ryan DA  Rose PL  Shaw RA 《American journal of veterinary research》2007,68(5):517-523

OBJECTIVE: To determine the feasibility of the use of Fourier-transform infrared (FTIR) spectroscopy within the midinfrared range to differentiate synovial fluid samples of joints with osteochondrosis from those of control samples. ANIMALS: 33 horses with osteochondrosis of the tarsocrural joint and 31 horses free of tarsocrural joint disease. PROCEDURES: FTIR spectroscopy of synovial fluid was used. Sixty-four synovial fluid samples from the tarsocrural joint were collected. Of these, 33 samples were from horses with radiographic evidence of osteochondrosis of the tarsocrural joint and 31 from control joints. Disease-associated features within infrared spectra of synovial fluid were statistically selected for spectral classification, and the variables identified were used in a classification model. Linear discriminant analysis and leave-one-out cross-validation were used to develop a classifier to identify joints with osteochondrosis. RESULTS: 12 significant subregions were identified that met the selection criteria. The stepwise discriminant procedure resulted in the final selection of 6 optimal regions that most contributed to the discriminatory power of the classification algorithm. Infrared spectra derived from synovial fluid of joints with osteochondrosis were differentiated from the control samples with accuracy of 77% (81% specificity and 73% sensitivity). CONCLUSIONS AND CLINICAL RELEVANCE: The disease-associated characteristics of infrared spectra of synovial fluid from joints with osteochondrosis may be exploited via appropriate feature selection and classification algorithms to differentiate joints with osteochondrosis from those of control joints. Further study with larger sample size including age-, breed-, and sex-matched control horses would further validate the clinical value of infrared spectroscopy for the diagnosis of osteochondrosis in horses.  相似文献   

Prediction of leaching fraction from soil properties,irrigation water and rainfall     

R. J. Shaw  P. J. Thorburn 《Irrigation Science》1985,6(2):73-83

Summary Fine textured soils (> 40% clay) form a major proportion of irrigated soils in northeastern Australia. More than half these soils are irrigated with groundwater, some of which has high salinity (electrical conductivity > 2.9 mS cm^–1). A simple prediction of salt leaching was sought to aid in land management decisions.An empirical model of leaching fraction is presented based on rainfall and easily measured soil properties related to hydraulic conductivity. The model is based on data from 766 soils. To account for the complexity of interactions between soil properties, the data was stratified into groups based on clay content and mineralogy (expressed here as CEC/clay ratio). This allowed simple linear regressions using ESP and rainfall to be developed to predict leaching fraction.When applied to irrigated soils, a salinity correction term (EC_{rain+irrigation}/EC_rain) was used to account for the flocculation effects of the increased salinity of irrigation waters. The model gave good predictions of leaching fraction for two irrigation regions with widely differing soil properties (Fig. 4).  相似文献