Development of wooden portal frame structures with improved columns     

Masahiro Noguchi  Shinjiro Takino  Kohei Komatsu 《Journal of Wood Science》2006,52(1):51-57

In Japan, the lifetime cycle of most housing lasts around 20–30 years. A governing factor in this respect is poor durability due to old-fashioned use of the house. As a solution of this problem, houses can be built with a skeleton structure that allows free partition of spaces by future owners. To develop the skeleton structure effectively, multistory frames with spans of 6 to 10 m are required. For this reason, attention has been focused on the behavior of multistory timber frame structures. In this article, two types of wooden portal frame structures are proposed. Both structures have improved vertical columns with short horizontal members glued in. The aim of this study was to investigate structurally effective solutions with these types of columns. The first type of the new structure changed the location of the moment-transmitting ductile connection with the improved columns. The second type of structure used an extended panel zone. Nine portal frame specimens were tested. The stiffness values were improved by around 1.7 and 3.5 times when compared with the control, and the strength was improved by around 1.25 and 1.45 times.  相似文献   

The critical stress in various stress levels of bending member on fire exposure for mechanical graded lumber     

Anita?Firmanti  Bambang?Subiyanto  Shinjiro?Takino  Shuichi?KawaiEmail author 《Journal of Wood Science》2004,50(5):385-390

This study focused on the relationship between stress level and the fire resistance of structural lumber. The 210 samples were prepared from 15-year-old trees of Acacia mangium from the Forest Estate Plantation, Indonesia. Specimens were 20 ® × 20 (T) × 500mm (L) and were air-dried. Sixty samples were tested under four-point bending stress to obtain the modulus of elasticity (MOE) and the modulus of rupture (MOR) for classifying the stress grades of the remaining 150 samples. The tests were performed using a four-point load bending position at various stress level while the specimens were exposed to fire along the shear-free region. Time to failure was affected by the stress levels in an exponential trend. Despite changes in stress level, charring rate, and time to failure, the critical stress of a member from the same species was similar. The fire performance under applied load could be predicted by using simplified fire-testing methods. The developed testing apparatus is valid for evaluating the small-scale fire resistant behavior of structural lumber in bending.  相似文献   

Utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of walleye pollock Theragra chalcogramma   总被引：3，自引：0，他引：3  

Nobuyuki Ohkubo  Sayumi Sawaguchi  Tomonori Hamatsu  Takahiro Matsubara 《Fisheries Science》2006,72(3):620-630

ABSTRACT:   To elucidate the utilization of the major yolk nutrient stocks in eggs and larvae of walleye pollock Theragra chalcogramma , the contents of free amino acids (FAA), the major yolk protein (180 kDa lipovitellin originated from vitellogenin B in ovulated eggs: oLv B), and lipids were measured. Most eggs hatched 18 days after fertilization at 5°C, and all larvae absorbed almost all their yolk mass by 28 days. The total FAA content showed no change during the first 6 days, and then decreased to 28% of the initial level by 18 days. The oLv B contents, measured by an enzyme-linked immunosorbent assay using a specific antiserum against oLv B, gradually decreased from 6 to 18 days, followed by a rapid decline. The content of phospholipids (PL) and triacylglycerols (TG) showed no marked change until hatching, and then decreased until disappearance of yolk sac. From these results, it is proposed that there are two main periods for nutrient utilization in embryos and larvae of walleye pollock. In the first period, FAA was mainly utilized until 18 days after fertilization. Active utilization of oLv B and lipids (PL and TG) instead of FAA occurred during the second period from 18 to 28 days.  相似文献   

Does the deep brachial artery exist in domestic animals?     

T Suzuki  M Ohkubo 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(3):469-472

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Validation of a food-frequency questionnaire for cohort studies in rural Japan     

Ogawa K  Tsubono Y  Nishino Y  Watanabe Y  Ohkubo T  Watanabe T  Nakatsuka H  Takahashi N  Kawamura M  Tsuji I  Hisamichi S 《Public health nutrition》2003,6(2):147-157

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Understory thinning reduces wood-production efficiency and tree species diversity in subtropical forest in southern Japan     

Shinjiro Fujii  Yasuhiro Kubota 《Journal of Forest Research》2011,16(3):253-259

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Distribution patterns of soluble salts and gypsum in soils under large-scale irrigation agriculture in Central Asia     

Shinya FUNAKAWA  Reiji SUZUKI  Shinjiro KANAYA  Elmira KARBOZOVA-SALJNIKOV  Takashi KOSAKI 《Soil Science and Plant Nutrition》2007,53(2):150-161

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Effects of Curtailment of Nitrogen Fertilizer on Biological Properties and Tea Leaf Yield in Acid Tea Field Soils     

Shinjiro Kanazawa  Gi-Hong An  Toshiyuki Kaizu 《Soil Science and Plant Nutrition》2005,51(5):675-677

In Japan, the soils in tea fields are further acidified by heavy application of nitrogen fertilizer. In the long-term fertilizer application fields, available P, total carbon and nitrogen contents had the highest in the Multi-F plot, followed by the 98-D.F plot and the smallest in the 95-D.F plot. Soil enzymes activity had the highest the Multi-F plot. It was estimated that all of the enzyme activity were often corresponded with the results of the organic matter and the available P contents. The number of fungi under acidic condition had the close relation since change of these enzymes activity. It was evident that fungi were participated in carbon, nitrogen and phosphorus metabolism in acid tea field soils. It became clear that the number of fungi and enzymes activity decreased corresponding well with the available P and the organic matter contents depend on fertilization management. Moreover in case of qualities of green tea, generally difference between experimental plots and Multi-F plot were to be small. It was indicated that influence to the yields by curtailment of the amount of nitrogen fertilization was small.  相似文献   

Labeled avidin-biotin enzyme-linked immunosorbent assay for detecting antibody to infectious laryngotracheitis virus in chickens   总被引：1，自引：0，他引：1  

Y Ohkubo  K Shibata  T Mimura  I Takashima 《Avian diseases》1988,32(1):24-31

A labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detecting antibody to infectious laryngotracheitis (ILT) virus in chicken sera was developed and compared with ordinary ELISA. Purified ILT virus, biotin-labeled anti-chicken IgG rabbit IgG conjugate, and horseradish-peroxidase-labeled avidin were used in the LAB-ELISA. When sera from farm chickens were tested by serum neutralization (SN) and two kinds of ELISA, the correlation rate between SN and LAB-ELISA was 50/50 (100%), and that between SN and ordinary ELISA was 39/50 (78%). In LAB-ELISA, all of the sera that were antibody-negative by SN had low absorbance (A) values (below 0.05), and the A values were closely correlated with the SN indexes. In ordinary ELISA, however, the sera antibody-negative by SN had various A values ranging from 0.06 to 0.32. LAB-ELISA had much lower nonspecific reactions than ordinary ELISA against sera from ILT-negative chickens, even when chickens were 30 weeks old. ILT antibody production after ILT vaccination could be detected by LAB-ELISA. A values peaked 5 weeks postinoculation and were maintained for 17 weeks.  相似文献   

Effects of various selective phosphodiesterase inhibitors on carbachol-induced contraction and cyclic nucleotide contents in guinea pig taenia coli     

Kaneda T  Shimizu K  Urakawa N  Nakajyo S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1047-1052

Effects of various selective phosphodiesterase (PDE) inhibitors on muscle contractility and cyclic nucleotide contents in guinea pig taenia coli were investigated. Forskolin and sodium nitroprusside inhibited carbachol (CCh)-induced contraction in a concentration-dependent manner. Various selective PDE inhibitors, vinpocetine (type 1), erythro -9-(2-hydroxy-3-nonyl)adenine (EHNA, type 2), milrinone (type 3), Ro20-1724(type 4) and zaprinast (type 5) inhibited CCh-induced contraction in a concentration-dependent manner, but the inhibition of milrinone was noticeably smaller than that of the other PDE inhibitors. The rank order of potency was zaprinast > vinpocetine > EHNA > Ro20-1724 > milrinone. In the presence of CCh (0.3 microM), vinpocetine and Ro20-1724 both increased cAMP content, but not cGMP. By contrast, EHNA and zaprinast both increased cGMP content, but not cAMP. Pretreatment with ODQ (30 microM), a soluble guanylyl cyclase inhibitor, decreased the inhibition of CCh-induced contraction by EHNA or zaprinast. Pretreatment with SQ22536 (100 microM), an adenylyl cyclase inhibitor, decreased the inhibition of CCh-induced contraction by vinpocetine or Ro20-1724. In conclusion, it was indicated that vinpocetine- or Ro20-1724-induced relaxation was correlated with cAMP but EHNA- or zaprinast- induced relaxation was correlated with cGMP.  相似文献