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Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding 总被引：46，自引：0，他引：46
Inter simple sequence repeat (ISSR)-PCR is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. It is a simple and quick methodthat combines most of the advantages of microsatellites (SSRs) andamplified fragment length polymorphism (AFLP) to the universality ofrandom amplified polymorphic DNA (RAPD). ISSR markers are highlypolymorphic and are useful in studies on genetic diversity, phylogeny, genetagging, genome mapping and evolutionary biology. This review providesan overview of the details of the technique and its application in geneticsand plant breeding in a wide range of crop plants. 相似文献
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Sahiner Nurettin Farooq Muhammad Rehman Saif ur Sagbas Selin Sahiner Mehtap Siddiq Mohammad Aktas Nahit 《Water, air, and soil pollution》2017,228(10):1-16
Water, Air, & Soil Pollution - Sustainable groundwater quality has become a major concern for the agro-based country like Bangladesh. Integrated approaches of various irrigation water quality... 相似文献
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Akkareddy Srividhya Lakshminarayana R. Vemireddy Sakile Sridhar Mudduluru Jayaprada Puram V. Ramanarao Arremsetty S. Hariprasad Hariprasad K. Reddy Ghanta Anuradha Ebrahimali Siddiq 《Journal of Crop Science and Biotechnology》2011,14(1):45-56
Moisture stress is the major constraint to rice production and its stability in rainfed, mainly irrigated, and aerobic environments.
Identification of genomic regions conferring tolerance to stress would improve our understanding of the genetics of stress
response and result in the development of drought tolerant cultivars. In the present study, quantitative trait loci for drought
response related traits and as well as grain yield were identified using a set of 140 recombinant inbred lines derived from
a cross between the popular high-yielding variety, IR64 and the landrace, INRC10192. A total of 36 QTL were identified for
grain yield and its components under control and stress conditions. Strikingly, a QTL cluster flanked by the markers RM38
and RM331 on chromosome 8 was found to be associated with grain yield, plant height, no. of productive tillers, chaffy grains,
and spikelet fertility on secondary rachis and biomass under stress treatment. The genomic regions associated with these QTL
under drought stress will be useful for the development of marker-based breeding for drought tolerant, high-yielding varieties
suited to drought-prone areas. 相似文献
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Vijay Yepuri Malathi Surapaneni Vijaya Sudhakar Rao Kola L. R. Vemireddy B. Jyothi V. Dineshkumar G. Anuradha E. A. Siddiq 《Journal of Crop Science and Biotechnology》2013,16(2):93-103
A total of 16,619 ESTs sequences (SSRs) of sesame (Sesamum indicum L.) were mined from Genbank. From sequences, 156 primer pairs were designed and characterized to determine the diversity among 49 sesame accessions. Twenty SSRs were found to be polymorphic and the number of alleles ranged from two to five per locus. The allele size varied from 101 to 399 bp. The average PIC value of the 20 SSR loci was 0.72 ranging from 0.49 (SEM-12-68) to 0.90 (SEM-12-27). Dendrogram analysis grouped the 49 genotypes into five separate clusters exhibiting a genetic similarity coefficient from 0.59 to 1.0. Hence, these EST-derived SSRs markers could be useful in assessing the diversity of sesame accessions and could also help in identifying diverse parents for sesame improvement programs. 相似文献
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