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Harms CA Maggi RG Breitschwerdt EB Clemons-Chevis CL Solangi M Rotstein DS Fair PA Hansen LJ Hohn AA Lovewell GN McLellan WA Pabst DA Rowles TK Schwacke LH Townsend FI Wells RS 《Veterinary research》2008,39(6):59
We present prevalence of Bartonella spp. for multiple cohorts of wild and captive cetaceans. One hundred and six cetaceans including 86 bottlenose dolphins (71 free-ranging, 14 captive in a facility with a dolphin experiencing debility of unknown origin, 1 stranded), 11 striped dolphins, 4 harbor porpoises, 3 Risso's dolphins, 1 dwarf sperm whale and 1 pygmy sperm whale (all stranded) were sampled. Whole blood (n = 95 live animals) and tissues (n = 15 freshly dead animals) were screened by PCR (n = 106 animals), PCR of enrichment cultures (n = 50 animals), and subcultures (n = 50 animals). Bartonella spp. were detected from 17 cetaceans, including 12 by direct extraction PCR of blood or tissues, 6 by PCR of enrichment cultures, and 4 by subculture isolation. Bartonella spp. were more commonly detected from the captive (6/14, 43%) than from free-ranging (2/71, 2.8%) bottlenose dolphins, and were commonly detected from the stranded animals (9/21, 43%; 3/11 striped dolphins, 3/4 harbor porpoises, 2/3 Risso's dolphins, 1/1 pygmy sperm whale, 0/1 dwarf sperm whale, 0/1 bottlenose dolphin). Sequencing identified a Bartonella spp. most similar to B. henselae San Antonio 2 in eight cases (4 bottlenose dolphins, 2 striped dolphins, 2 harbor porpoises), B. henselae Houston 1 in three cases (2 Risso's dolphins, 1 harbor porpoise), and untyped in six cases (4 bottlenose dolphins, 1 striped dolphin, 1 pygmy sperm whale). Although disease causation has not been established, Bartonella species were detected more commonly from cetaceans that were overtly debilitated or were cohabiting in captivity with a debilitated animal than from free-ranging animals. The detection of Bartonella spp. from cetaceans may be of pathophysiological concern. 相似文献
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Ghulam Sarwar Solangi Filitsa Karamaouna Dimitris Kontodimas Panagiotis Milonas Mohammad Khan Lohar Ghulam Hussain Abro Riaz Mahmood 《Phytoparasitica》2013,41(2):213-219
The effect of high temperature on survival and longevity of Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) female adults was studied in the laboratory by daily exposure of the beetles to 35°C (mean r.h. 52%) combined with 1-, 2- and 3-h intervals of thermal treatment at 38, 40, 42 and 44°C (r.h. 46–48%). Fifty percent of females survived until the 12th–13th day of the thermal treatment at 38°C (depending on the exposure time), the 2nd–10th day at 40°C for 1–2 h exposure and the 4th–5th day at 42°C for 1 h exposure. Survival was 25–38% on the 1st–2nd day of the thermal treatment at 40, 42 and 44°C for 3, 2 and 1 h of exposure, respectively. Three hours of exposure of female C. montrouzieri at 42°C and 2 h exposure at 44°C were lethal for the beetles. Mean longevity of the predator was 33.13?±?1.22 days at 35°C and was significantly shorter as the temperature of the thermal treatments increased from 38o to 44°C. These results suggest that daily temperature regimes of 35°C combined with 38–40°C for 1–3 h are marginal for the survival and longevity of the C. montrouzieri, whereas temperatures above 42°C are lethal even at short exposure. These results can partly justify the inability of C. montrouzieri to be permanently established in Pakistan environments and could contribute to a pre-evaluation assay of its potential adaptability in such environmental systems. 相似文献
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Mitchell MA Solangi MA Clemons-Chevis CL Vanderpool D Romagnoli M Hoffland T Jowett P 《American journal of veterinary research》2008,69(7):900-903
OBJECTIVE: To determine the correlation between plasma iron concentrations and gastric pH in a population of captive Atlantic bottlenose dolphins (Tursiops truncatus). ANIMALS: 6 adult female dolphins that ranged from 16 to 30 years of age. PROCEDURES: Blood and gastric samples were collected from each dolphin to allow measurement of plasma iron concentrations and gastric pH, respectively. Samples were collected each month for 12 months. RESULTS: Within each dolphin, plasma iron concentrations and gastric pH did not differ significantly over time. There was a strong negative correlation (r = -0.85) between plasma iron concentration and gastric pH, which suggested that dolphins with a lower gastric pH had a higher plasma iron concentration. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of results reported here suggested that gastric pH may play an important role in iron absorption in dolphins. 相似文献
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Buddington KK Holmes WE Clemons-Chevis CL Solangi MA Vanderpool D Buddington RK 《American journal of veterinary research》2006,67(6):931-935
OBJECTIVE: To measure concentrations of sucrose in the serum of captive dolphins after oral administration of a sucrose solution and determine the suitability of this method for use as a test to detect gastric ulcers. ANIMALS: 8 adult captive bottlenose dolphins (Tursiops truncatus). PROCEDURES: Blood samples were collected from the ventral fluke vein of dolphins before and 45 minutes after oral administration of 500 mL of solution containing 25 or 50 g of sucrose; oral administration was achieved by use of gastric intubation. Serum was separated, diluted in a solution of 90% acetonitrile-to 10% water that contained 10 ng of an internal standard (trichlormethiazide)/microL, mixed, and centrifuged. Supernatant was analyzed by use of liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). RESULTS: Serum sucrose concentrations of dolphins were at or less than the limits of detection before oral administration. Values after administration of sucrose solution varied among dolphins and were higher and more variable after administration of 50 g, compared with concentrations after administration of 25 g. CONCLUSIONS AND CLINICAL RELEVANCE: Serum sucrose concentrations in samples collected during routine health evaluations of captive dolphins can be reliably measured by use of LC-MS-MS. Correlating serum sucrose concentrations with endoscopic observations of the gastric mucosa of dolphins will validate this approach for use in screening for the prevalence and severity of gastric ulcers and determining the efficacy of treatment regimens. 相似文献
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Wangsheng Zhao Tajmal Hussain Solangi Yitao Wu Xiankang Yang Chuanfei Xu Hongmei Wang Xuxin Zheng Xin Cai Jiangjiang Zhu 《Reproduction in domestic animals》2021,56(4):555-576
The epididymis is the site of post-testicular sperm maturation, which constitutes the acquisition of sperm motility and the ability to recognize and fertilize oocytes. The role of miRNA in male reproductive system, including the control of different steps leading to proper fertilization such as gametogenesis, sperm maturation and maintenance of male fertility where the deletion of Dicer in mouse germ cells led to infertility, has been demonstrated. The identification of miRNA expression in a region-specific manner will therefore provide valuable insight into the functional differences between the regions of the epididymis. In this study, we employed RNA-seq technology to explore the expression pattern of miRNAs and establish some miRNAs of significant interest with regard to epididymal sperm maturation in the CY epididymis. We identified a total of 431 DE known miRNAs; 119, 185 and 127 DE miRNAs were detected for caput versus corpus, corpus versus cauda and caput versus cauda region pairs, respectively. Our results demonstrate region-specific miRNA expression in the CY epididymis. The GO and KEGG enrichment for the predicted target genes indicated the functional values of miRNAs. Furthermore, we observed that the expression of miR-200a was downregulated in the caput, compared with cauda. Since the family of miR-200 has previously been suggested to contribute to the distinct physiological function of sperm maturation in epididymis of adult rat, we speculate that the downregulation of miR-200a in CY caput epididymis may play an important role of sperm maturation in the epididymis of CY. Therefore, our findings may not only increase our understanding of the molecular mechanisms regulated by the miRNA functions in region-specific miRNA expression in the CY epididymis, it could provide a valuable information to understand the mechanism of male infertility of CY. 相似文献
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Rasheed Adnan Wassan Ghulam Mustafa Khanzada Hira Solangi Abdul Malik Han Ruicai Li Huijie Bian Jianmin Wu Ziming 《Genetic Resources and Crop Evolution》2021,68(5):1889-1903
Genetic Resources and Crop Evolution - Aluminium toxicity is adversely affecting the rice growth and production in acidic soils of developing countries. Rice is popular and stable crop which meets... 相似文献
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Qurban Ali Rind Abdul Ghani Lanjar Abdul Ghafoor Rahoo Ghulam Sarwar Solangi 吕荣华 付岗 覃武 《南方农业学报》2019,50(9):1973-1980
[目的]探讨亚金跳小蜂对不同生育期扶桑绵粉蚧的影响,为今后寄生蜂的饲养和推广及扶桑绵粉蚧的生物防治提供理论参考.[方法]分别以4个龄期的15头扶桑绵粉蚧、30头3龄幼虫和成虫为试虫进行选择性和非选择性试验,每虫笼放入1对亚金跳小蜂,每天记录寄主和寄生蜂的存活情况.[结果]亚金跳小蜂的最大寄生率和扶桑绵粉蚧的最大僵虫量均在扶桑绵粉蚧3龄若虫期出现,而最低值均在其2龄若虫期出现.在选择和非选择试验中,亚金跳小蜂雌虫的寿命差异不显著(P>0.05).在以扶桑绵粉蚧2龄若虫为试验对象的选择性和非选择性试验中,雌性亚金跳小蜂的生命周期分别为12.69±0.19和13.24±0.40 d.在以扶桑绵粉蚧3龄若虫为试验对象的选择性和非选择性试验中,雌性亚金跳小蜂的生命周期分别为15.51±0.28和15.12±0.14 d.寄生蜂的发育周期因寄主龄期而有明显差异,在扶桑绵粉蚧3龄若虫期寄生的亚金跳小蜂生命周期最长,而在扶桑绵粉蚧3龄若虫期寄生的亚金跳小蜂生命周期最短.[结论]作为寄生蜂的亚金跳小蜂在寄主扶桑绵粉蚧3龄若虫期的成虫量最大,在2龄若虫期的成虫量最小. 相似文献
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