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Giffoni JH de Almeida CE dos Santos SO Ortega VS de Barros AT 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(4):485-492
A study was designed to examine the effect of 65% permethrin spot-on on the prevalence of canine visceral leishmaniasis and the abundance of sand flies in two neighborhoods in Corumbá, Mato Grosso do Sul, Brazil known to have a high prevalence of visceral leishmaniasis. An enrollment survey was conducted to determine the prevalence of visceral leishmaniasis. Area residents were provided with information about the project; the study area was defined, and all dogs (160 in Cristo Redentor and 230 in Popular Velha) identified in the study area were enrolled. Three 65% permethrin spot-on treatments (June 15-25, July 13-15, and August 10-12) were administered to 150, 110, and 99 dogs, respectively, in Popular Velha, according to label recommendations. Dogs in Cristo Redentor were untreated controls. Visceral leishmaniasis was diagnosed periodically by indirect immunofluorescence assay. A reduction in canine visceral leishmaniasis prevalence was observed at the Popular Velha site. The infection rate for treated dogs 1 month following the final treatment was approximately 50% reduced from that observed before treatment(19.3% vs 9.6%). Conversely, the infection rate at the control site was more than 80% higher at the September sampling than that observed pretreatment (4.1% vs 7.4%). Similar numbers of sand flies were captured and identified from both sites throughout the study. The results suggest that regular use of 65% permethrin during months of high risk for canine visceral leishmaniasis can be a useful strategy for reducing the prevalence of this disease in hyperendemic areas. It should be stressed, however, that the success of this strategy depends not only on the efficacy of the product itself but also on the adoption of other control measures and on economic variables, considering the low purchasing power of the populations living in higher-risk neighborhoods. 相似文献
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Belarbi M Bendimerad S Sour S Soualem Z Baghdad C Hmimed S Chemat F Visioli F 《Journal of agricultural and food chemistry》2011,59(16):8667-8669
The olive tree had been domesticated during the early Neolithic in the Near East, and more than 1000 different cultivars have been identified to date. However, examples of wild olive trees (Olea europaea oleaster) can still be found in the Mediterranean basin. Evidence of oleaster use for oil production can be found in historical and sacred texts, such as the Odyssey, the Holey Koran, and the Holey Bible. While the nutritional and healthful properties of olive oil are actively being explored, there are no data on the human actions of oleaster oil. Therefore, we investigated the effect of prolonged, i.e., 1 month, consumption of oleaster oil on the lipid profile of a 40 healthy Algerian subjects (aged 27.9 ± 3.85 years), as compared to nonconsumers from the same area. Plasma urea, creatinine, and uric acid concentrations and glycemia did not significantly differ, at the end of the study, between controls and oleaster-oil-supplemented subjects. Conversely, we recorded significant decreases of plasma triglyceride concentration (-24.8%; p < 0.05), total cholesterol (-12.13%; p < 0.05), and low-density lipoprotein-cholesterol (LDL-C) (-24.39%; p < 0.05) in oleaster-oil-treated subjects. Concomitantly, high-density lipoprotein-cholesterol (HDL-C) concentrations were significantly increased (17.94%; p < 0.05) by oleaster oil administration. In conclusion, we show that oil obtained from feral olive trees, i.e., oleasters, improves the plasma lipid profile of healthy volunteers. 相似文献
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Samira Gilanchi Banafshe Esmaeilzade Akram Eidi Mahmood Barati Soraya Mehrabi Fatima Moghani Ghoroghi Maliheh Nobakht 《Iranian Biomedical Journal》2014,18(3):136-142
Background: The seladin-1 (selective Alzheimer disease indicator-1), also known as DHCR24, is a gene found to be down-regulated in brain region affected by Alzheimer disease (AD). Whereas, hair follicle stem cells (HFSC), which are affected in with neurogenic potential, it might to hypothesize that this multipotent cell compartment is the predominant source of seladin-1. Our aim was to evaluate seladin-1 gene expression in hair follicle stem cells. Methods: In this study, bulge area of male Wistar rat HFSC were cultured and then characterized with Seladin-1 immunocytochemistry and flow cytometry on days 8 to 14. Next, 9-11-day cells were evaluated for seladin-1 gene expression by real-time PCR. Results: Our results indicated that expression of the seladin-1 gene (DHCR24) on days 9, 10, and 11 may contribute to the development of HFSC. However, the expression of this gene on day 11 was more than day 10 and on 10th day was more than day 9. Also, we assessed HFSC on day 14 and demonstrated these cells were positive for β-ш tubulin, and seladin-1 was not expressed in this day. Conclusion: HFSC express seladin-1 and this result demonstrates that these cells might be used to cell therapy for AD in future. Key Words: Seladin-1 (selective Alzheimer disease indicator-1), Alzheimer disease (AD), Hair follicle stem cells 相似文献
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Molecular and morphological characterization of Golestan (Iran) olive ecotypes provides evidence for the presence of promising genotypes 总被引：1，自引：1，他引：0
Soraya Mousavi Mehdi Hosseini Mazinani Kazem Arzani Abbas Ydollahi Saverio Pandolfi Luciana Baldoni Roberto Mariotti 《Genetic Resources and Crop Evolution》2014,61(4):775-785
Unidentified olive plants naturally grow in the Golestan province of Iran, on different soils and under climates spanning from sub-temperate to desert conditions, represented by single trees or groups of few trees. We collected samples from representative sites and analyzed them by simple sequence repeat markers in order to determine their identity and their relationships to prominent Iranian and Mediterranean reference cultivars. Population structure analysis separated these ecotypes from Mediterranean and, surprisingly, from all Iranian cultivars, the parentage test excluded their direct contribution as candidate parents or offspring of cultivars, and they also showed a high level of admixture. Their differentiation from cultivated olives may be attributed to different factors: they could represent wild plants or could derive from natural dissemination of ancestral cultivated trees. Their survival up to now may be due to the fact that most of them are grown on sacred sites such as necropolis. Anyhow, the adaptation to strong environmental stresses, and their fruit size and oil content make the olive Golestan ecotypes a valuable source of genetic variation previously uncharacterized and currently threatened with extinction. 相似文献
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Antonio Ruiz María Carmen Muñoz José Manuel Molina Carlos Hermosilla Marisa Andrada Pedro Lara Elisa Bordón Davinia Pérez Adassa María López Lorena Matos Aránzazu Carmen Guedes Soraya Falcón Yaiza Falcón Sergio Martín Anja Taubert 《Veterinary parasitology》2014,199(1-2):8-17
Caprine coccidiosis, affecting mainly young goat kids around the weaning period, is worldwide the most important disease in the goat industry. Control of caprine coccidiosis is increasingly hampered by resistances developed against coccidiostatic drugs leading to an enhanced need for anticoccidial vaccines. In the current study we conducted an oral immunization trial with live attenuated sporulated Eimeria ninakohlyakimovae oocysts. Sporulated E. ninakohlyakimovae oocysts were attenuated by X-irradiation technique. The experimental design included a total of 18 goat kids divided into the following groups: (i) animals immunized with attenuated E. ninakohlyakimovae oocysts at 5 weeks of age and challenged 3 weeks later with non-irradiated homologous oocysts (group 1); (ii) animals infected with non-attenuated E. ninakohlyakimovae oocysts at 5 weeks of age and challenged 3 weeks later with non-attenuated homologous oocysts (group 2); (iii) animals primary-infected with untreated E. ninakohlyakimovae oocysts at 8 weeks of age (control of the challenge infection, group 3); (iv) non-infected control animals (group 4). Goat kids immunized with live attenuated E. ninakohlyakimovae oocysts (group 1) excreted significantly less oocysts in the faeces (95.3% reduction) than kids infected with non-attenuated ones (group 2). Furthermore, immunization with live but attenuated oocysts resulted in ameliorated clinical coccidiosis compared to goat kids infected with untreated oocysts (group 2) and resulted in equally reduced signs of coccidiosis after challenge infection compared to acquired immunity driven by non-attenuated oocysts. Overall, the present study demonstrates for the first time that live attenuated E. ninakohlyakimovae oocysts orally administered showed almost no pathogenicity but enough immunogenicity in terms of immunoprotection. Importantly, vaccinated animals still shed low amounts of oocysts, guaranteeing environmental contamination and consecutive booster infections to sustain ongoing immunity. 相似文献
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A 6‐year‐old female, spayed Collie was referred to the Western College of Veterinary Medicine for a 12‐month history of a progressive right corneal mass. A superficial keratectomy was performed and histopathology revealed a corneal melanocytoma with complete excision. There has been no recurrence of the neoplasm to date (12 months). This is the first known report of an isolated corneal melanocytoma in a canine. 相似文献
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Soraya Ruamrungsri Takuji Ohyama Takamitsu Konno Taro Ikarashi 《Soil Science and Plant Nutrition》2013,59(4):809-820
Narcissus cv. “Garden Giant” bulbs were grown in N, P, K, Ca, Mg, or Fe-deficient solutions and compared with bulbs grown in the control solution containing all these nutrients. Plants were sampled at 4 stages: (I) at planting, (II) at sprouting, (III) after flower senescence and/or after visible deficiency symptoms developed, and (IV) at lifting. Observation of visible deficiency symptoms showed that leaves of narcissus displayed chlorosis in the —N, —Mg, and —Fe treatments, while roots were most susceptible to Ca-deficient conditions. Root tips in the —Ca treatment showed brown in followed by root rot. In the —N treatment, shoot growth was markedly retarded and leaves were small and yellow. On the other hand, visible deficiency symptoms were not evident in the —P treatment except for early senescence. K deficiency symptoms were also not evident. Narcissus flowers were not affected by the mineral deficiencies and mineral contents in full-bloom flowers were not different between samples. In the control plants, there was a large accumulation of N in the roots at sprouting and the content decreased thereafter. Large amounts of K accumulated in roots more than in any other organs. On the other hand the Ca content was high in the tunic and Ca in the scales was hardly mobilized thoughout the growth period. A large proportion of each element eventually accumulated in new inner scales whereas only a small fraction in the old outer scales. The -N, -Ca, and -Mg treatments severely depressed dry matter accumulation, unlike the -P, -K, and -Fe treatments. The -N treatment did not affect the concentration of other minerals, but the -P treatment tended to decrease N, K, Ca, and Mg concentrations. On the other hand, the -K treatment increased Ca and Mg concentrations. -Ca caused an increase in Mg concentration and -Mg raised Ca concentration. These results may be due to compensation effects of cation absorption for the maintenance of the cation/anion balance. 相似文献
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Chamaiporn Anuwong Norikuni Ohtake Kuni Sueyoshi Soraya Ruamrungsri 《The Journal of Horticultural Science and Biotechnology》2013,88(5):501-507
SummaryCurcuma alismatifolia rhizomes are exported to world markets for ornamental use. Rhizome size and the number of storage roots affected shoot sprouting, plant growth, and inflorescence yield and quality, indicating an important role for storage food reserves such as the carbohydrates and proteins in these organs. An understanding of the proteins used for growth and development in rhizomes and in storage roots is lacking, so changes in the storage proteins in these organs were investigated at the planting stage, the sprouting stage, the second-leaf stage, the flowering stage, and the dormancy stage. The protein concentrations in both these organs decreased rapidly until the flowering stage in order to support the initial growth of roots and shoots. The intensity of Coomassie Brilliant Blue staining of tissues was stronger in rhizomes than in storage roots. Protein staining was present in the cytosol and in the cell walls in rhizomes, and was intense in small particle-like protein bodies in the cytosol. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles showed that peptides of 10.6 kDa and 12.0 kDa were the two major soluble proteins in rhizomes and were different from the proteins in storage roots.The 10.6 kDa and 12.0 kDa bands contained five peptides and one peptide, respectively, when separated by 2D-PAGE. The amino acid sequences of these six peptides were not homologous to any known proteins using BLASTP or FASTA analysis. The N-terminal amino acid sequences of these two polypeptides did not start with methionine, indicating that they had undergone posttranslational modification. Knowledge of the major storage proteins in the rhizomes and storage roots of C. alismatifolia may provide information to improve N-fertilisation. 相似文献