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The effect was studied of nitrates and nitrites in drinking water on the methemoglobin level in blood and pathomorphological changes in fourteen-day-old pheasant chickens. The concentrations of 500 ppm of NO3- and 15 ppm NO2- in the drinking water were not lethal, they caused only the increase in the methemoglobin in blood to 7.1% (NO3-) and 16.5% (NO2-). The pheasants exposed to NO3- suffered from hyperaemia of liver, kidneys and mucosa of the small intestine and from the multiplication of the eosinophilic granulocytes in the villus stroma. The exposition to NO2- resulted in the non-specific dystrophic changes in liver and kidneys and in the villus edema of the small intestine. Lethal levels of nitrates and nitrites in drinking water were estimated in relation to the age of pheasants. 相似文献
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Strnad J 《Science (New York, N.Y.)》1966,154(3746):259-261
Moments of inertia and quadrupole moments of deformed even even nuclei were calculated in the closely packed spheron model. 相似文献
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Matys Grygar Tomáš Sedláček Jan Bábek Ondřej Nováková Tereza Strnad Ladislav Mihaljevič Martin 《Water, air, and soil pollution》2012,223(2):739-753
Regional contamination by Pb and Zn in southern Moravia (south-east part of the Czech Republic) in the twentieth century was
analysed in Brno Dam lake sediments and in floodplain sediments of the Morava River near Strážnice. The age model for the
Brno Dam lake sediments has been obtained by 137Cs (maxima corresponding to the nuclear tests in atmosphere and the 1986 Chernobyl accident) and the construction of the dam
(1940); the time constraints for the Morava River sediments was the erection of flood defences (1930s) and 210Pb dating. In the case of floodplain sediments, profiles exhibiting post-depositional mobilisation of heavy metals by pedogenic
processes (gleying) must be excluded to reconstruct the history of contamination. There was a relatively fast joint onset
of Pb and Zn load since the early stages of industrialisation in the first half of the twentieth century, but then the concentrations
of these two metals developed in a different manner. Pb load only slightly increased till its peak in 1960s and 1970s. The
increase of Zn load was rather stepwise: Soon before 1930s (Morava River floodplain) and in 1940s and 1950s (in Brno Lake),
the relative contamination by Zn was much lower than during its peak in the 1970s to the present days. The offset of Pb and
Zn contamination curves could have been caused by three different artificial sources of these heavy metals. The temporal shift
of Pb and Zn loads can be used for dating sediments. 相似文献
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Bridges TJ Patel CK Strnad AR Wood OR Brewer ES Karlin DB 《Science (New York, N.Y.)》1983,219(4589):1217-1219
In carbon dioxide laser surgery of the vitreous a process of vaporization has been advocated. In this report syneresis, a thermal liquefaction of gel, is shown to be over ten times more efficient on an energy basis than vaporization. Syneresis of vitreous is experimentally shown to be a first-order kinetic process with an activation energy of 41 +/- 0.5 kilocalories per mole. A theory of laser surgery in which this figure is used agrees closely with results from laser experiments on human eye-bank vitreous. The syneresis of vitreous by carbon dioxide laser radiation could lead to a more delicate form of ocular microsurgery, and application to other biological systems may be possible. 相似文献
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Little is known about the immediate effect of high nitrogen (N), phosphorus (P) and potassium (K) application rates on sown grasslands cut twice per year. We asked how quickly plant species composition, biomass yield, biomass chemical properties and nutrient balance respond to N, P and K application. An experiment using unfertilized control, P, N, NP and NPK treatments was established on seven‐year‐old cut grassland in the Czech Republic in 2007 and monitored over four years. Annual application rates were 300 kg N ha?1, 80 kg P ha?1 and 200 kg K ha?1. The immediate response of plant species composition to N application was recorded and was found to be different to the response over the four years of the study period. Highly productive grasses (Dactylis glomerata, Festuca arundinacea and Phleum pratense) were promoted by N application in 2008 and then retreated together with legumes (Medicago sativa, Trifolium pratense and Trifolium repens) in all N treatments where the expansion of perennial forbs (Urtica dioica and Rumex obtusifolius) and annual weeds (Galinsoga quadriradiata, Impatiens parviflora, Lamium purpureum and Stellaria media) was recorded. At the end of the experiment, Festuca rubra was the dominant grass in the control and P treatment, and species richness was lowest in all treatments with N application. Mean annual dry‐matter yield over all years was 3.5, 3.9, 5.8, 5.6 and 6.8 t ha?1 in the control, P, N, NP and NPK treatments, respectively. Concentrations of N in the biomass ranged from 20.0 to 28.7 g kg?1 in the P and N treatments; concentrations of P ranged from 3.2 to 3.7 g kg?1 in the N and P treatments; and concentrations of K ranged from 24.1 to 34.0 g kg?1 in the NP and NPK treatments. The N:P, N:K and K:P ratios did not correctly indicate the nutrient limitation of biomass production, which was primarily N‐limited, and K‐limitation was only recorded for high production levels in treatments with N applications. On the basis of the nutrient‐balance approach, the balanced annual application rates were estimated as 140 kg N ha?1, 30 kg P ha?1 and 100 kg K ha?1. We concluded that high N, P and K application rates can very quickly and dramatically change species composition, biomass production and its chemical properties in sown cut grasslands. High N application rates can be detrimental for tall forage grasses and can support the spread of weedy species. 相似文献
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ABSTRACT: BACKGROUND: We have developed a new analytical approach for isolation and quantification of cytokinins (CK) in minute amounts of fresh plant material, which combines a simple one-step purification with ultra-high pressure liquid chromatography-fast scanning tandem mass spectrometry. RESULTS: Plant tissue samples (1-5 mg FW) were purified by stop-and-go-microextraction (StageTip purification), which previously has only been applied for clean-up and pre-concentration of peptides. We found that a combination of two reverse phases and one cation-exchange phase, was the best tool, giving a total extraction recovery higher than 80%. The process was completed by a single chromatographic analysis of a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) in 24.5 minutes using an analytical column packed with sub-2-microne particles. In multiple reaction monitoring mode, the detection limits ranged from 0.05 to 5 fmol and the linear ranges for most cytokinins were at least five orders of magnitude. The StageTip purification was validated and optimized using samples of Arabidopsis thaliana seedlings, roots and shoots where eighteen cytokinins were successfully determined. CONCLUSIONS: The combination of microextraction with one-step high-throughput purification provides fast, effective and cheap sample preparation prior to qualitative and quantitative measurements. Our procedure can be used after modification also for other phytohormones, depending on selectivity, affinity and capacity of the selected sorbents. 相似文献
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Tomáš Matys Grygar Jitka Elznicová Tereza Lelková Timea Kiss Márton Balogh Ladislav Strnad Tomáš Navrátil 《Journal of Soils and Sediments》2017,17(11):2596-2609