排序方式： 共有13条查询结果，搜索用时 47 毫秒
1.
以款冬叶片为试材,采用微波辅助法提取总黄酮,在单因素试验的基础上,采用正交实验法优化款冬叶片总黄酮提取工艺,同时以分光光度法测定提取物对1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O-·2)的清除作用。结果表明:款冬叶片总黄酮最佳提取条件为乙醇体积分数70%,微波提取时间3 min,微波功率500 W,料液比1∶15 g·mL^-1,在该条件下,总黄酮的提取率为0.91%。款冬叶片总黄酮的质量浓度与抗氧化活性呈一定的线性关系,当总黄酮质量浓度为0.069 8 mg·mL^-1时,对DPPH·、·OH和O-·2的清除率分别达84.21%、84.10%和68.32%,表明款冬叶片具有良好的抗氧化能力,可作为天然的抗氧化剂进一步的开发和利用。 相似文献
2.
为给奶牛营养代谢病等相关研究提供种子细胞,本试验从犊牛肾周脂肪组织中分离出脂肪干细胞(adipose-derived stem cells, ADSCs),采用组织剪碎法结合0.25%Ⅰ型胶原酶消化法消化、收集原代犊牛ADSCs,通过细胞形态学观察,使用免疫荧光染色法检测第5代ADSCs表面特异性蛋白,并使用CCK-8法测定第5、10代细胞生长曲线,观察第10代细胞的成脂、成骨分化能力。结果显示,分离出的犊牛ADSCs接种4 d后细胞呈成纤维细胞样生长;免疫荧光染色结果显示,分离的细胞Vimentin、CD34、CD44、CD90呈阳性,CD31、CD45呈阴性;该细胞在适当的诱导条件下可以定向分化为脂肪细胞和成骨细胞。本试验分离培养的细胞是犊牛脂肪干细胞,具有成脂、成骨分化能力。 相似文献
3.
采用国际上通用的标准喷塔法测定了4株玫烟色拟青霉和4株球孢白僵菌对菊小长管蚜的毒力。结果表明,4株拟青霉在21~902个孢子/mm2的接种剂量下引起的死亡率为17.4%~100.0%,4株白僵菌在10~646个孢子/mm2的接种剂量下引起的死亡率为37.3%~100.0%。接种后第5天各菌株的致死中剂量LC50分别126个孢子/ mm2 (Pfr116),442个孢子/ mm2 (Pfr612),213个孢子/ mm2 (Pfr153),234个孢子/ mm2 (Pfr2175),155个孢子/ mm2 (Bb2880),86个孢子/ mm2 (Bb2860),99个孢子/ mm2 (Bb2861)和138个孢子/ mm2 (Bb2879)。致死中时LT50从低浓度下的较大差异向高浓度下的较小差异渐近收敛,在150个孢子/ mm2的剂量下,玫烟色拟青霉不同菌株的LT50变化范围为4.5~7.7天,球孢白僵菌不同菌株的LT50变化范围为4.5~5.1天。综合比较,4株玫烟色拟青霉中以Pfr2175的毒力最强,4株球孢白僵菌中以Bb2860最优,具有杀蚜速度快、致死浓度低等特点,具有较大的开发应用潜力。 相似文献
4.
以不同浓度的GA3、6-BA和IAA浸种24 h,通过比较浸种后种子萌发以及幼苗生长各项指标来研究植物生长调节剂浸种对金荞麦种子发芽和幼苗生长的影响.结果表明,在本试验处理浓度范围内,150 mg/L的GA3浸种后种子发芽率、发芽指数、活力指数、平均鲜重、平均株高、平均根长、叶绿素含量、POD活力分别提高37.7％、39.6％、68.3％、75.6％、17.7％、20.5％、18.7％、68％.而用0.1 mg/L 6-BA和25 mg/L IAA浸种后各项指标与对照相比均显著增加.3种植物生长调节剂综合比较表明,150 mg/L的GA3对金荞麦种子萌发和幼苗生长作用效果最为理想. 相似文献
5.
当前,大数据作为新型生产要素,正引领着各行业的加速转型升级.数字农业作为大数据与农业深度融合的产物,已成为促进农业生产标准化、决策精准化、监督网络化的重要手段.近年来,安徽省数字农业快速发展,数字农业基础设施不断完善,农业生产数字化水平、农产品电商销售率不断提高,但安徽省数字农业仍存在发展不均衡、不充分的问题.对此,该文提出了完善数字农业公共服务机构设置、加强数字农业项目统筹规划、强化数据资源共享与利用、加强人才交流与培训等对策建议. 相似文献
6.
Kapil Kumar Gupta Mukesh Srivastava Vikrant Sudan Shanker Kumar Singh Soumen Choudhury Daya Shanker 《Tropical animal health and production》2018,50(6):1227-1230
The present study was designed to assess the deleterious effects of bovine tropical theileriosis on the cardiovascular system and the consequent myocardial involvement in young calves. Myocardial effects in parasitic diseases are often neglected. Hemolytic anemia, associated secondary hypoxia, and vasculitis are cardinal features of bovine theileriosis. In the present study, electrocardiogram (ECG) alongside serum cardiac troponin I (cTnI) and creatinine phosphokinase-myocardial band (CPK-MB) concentrations were analyzed in infected, treated, and control groups of young calves. Non-significant alterations were noticed in ECG. However, certain signs like sinus tachycardia, first-degree AV block, atrial premature complex, left atrial hypertrophy, and right atrial hypertrophy were found on consistent basis in infected calves. A significant increase in the serum concentration levels of cTnI and CPK-MB was noticed in infected calves followed by significant fall in both these biomarkers post treatment. cTnI and CPK-MB can definitely be used as myocardial markers in theileriosis-affected animals. 相似文献
7.
Nano-silver pulse treatments inhibit stem-end bacteria on cut gerbera cv. Ruikou flowers 总被引：2，自引：0，他引：2
Jiping Liu Shenggen He Zhaoqi Zhang Jinping Cao Peitao Lv Sudan He Guiping Cheng Daryl C. Joyce 《Postharvest Biology and Technology》2009,54(1):59-62
Nanometer-sized silver (Ag+) particles (NS) are used in various applications as an anti-microbial. Effects of NS (2–5 nm diam.) pulse solution treatments on vase life of cut gerbera (Gerbera jamesonii) cv. Ruikou flowers were investigated. Compared with the control [pulsed with deionised water (DI) and subsequently held in DI] pulsing for 24 h with 5 mg/L NS solution followed by holding in DI maintained water uptake and extended vase life. From in vitro and microscopy assessments, NS pulse treatment inhibited bacteria growth in the vase solution and at cut stem ends during the first 2 d of the vase period. 相似文献
8.
利用不同浓度NaCl处理过表达SbRFP1的马铃薯转基因株系和对照组培苗,结果显示5和8 g·L-1的NaCl胁迫时,转基因株系的株高、根长和鲜质量均显著超过非转基因对照。转录组测序发现F-box基因(PGSC0003DMG400013116)和GDSL(Gly-Asp-Ser-Leu)酯酶基因(PGSC0003DMG400007815)等在转基因株系中的表达量显著高于非转基因对照,且经NaCl诱导上调表达。用外源物质处理非转基因组培苗并检测差异基因的表达量,结果显示差异表达基因受水杨酸(SA)等多种物质和NaCl诱导表达。说明Sb RFP1可能是通过调控F-box和GDSL酯酶等胁迫相关基因的表达提高马铃薯组培苗的耐盐性。 相似文献
9.
Sudan Jebi Singh Ravinder Mahajan Reetika Salgotra Romesh K. 《Genetic Resources and Crop Evolution》2021,68(6):2281-2288
Genetic Resources and Crop Evolution - Brassica represents an agriculturally important and diverse group of oilseed crops with a long evolutionary history. Molecular markers played an important... 相似文献
10.
Sudan Vikrant Jaiswal Amit Kumar Shanker Daya Verma Amit Kumar 《Tropical animal health and production》2017,49(8):1793-1796
Rotat 1.2 variant surface glycoprotein (VSG) is considered to be an important VSG expressed in most of the isolates of Trypanosoma evansi. This makes the molecule an important candidate for both molecular- and serological-based detection of surra. There are ample reports of existence of this gene in isolates from cattle, buffalo, and camel across the world. Of late, there are reports of its absence from a fewer isolates of T. evansi of murine and wildlife origin. Search of literature revealed no reports from horses. The present communication presents the first report of molecular cloning and characterization of Rotat 1.2 VSG from horse isolate of T. evansi from semi-arid region of India. Alongside, the gene was compared with various other isolates across the world. Interestingly, the isolate was found to be closer to camel isolates from Egypt than the other known isolates from India and Kenya.
相似文献