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1.
Kuo PC Lin MC Chen GF Yiu TJ Tzen JT 《Journal of agricultural and food chemistry》2011,59(7):3214-3219
The methanol extract of sesame (Sesamum indicum) seeds was fractionated and purified with the assistance of conventional column chromatography to afford 29 compounds including seven furofuran lignans. Among these isolates, (+)-samin (1) was obtained from the natural source for the first time. In addition, (-)-asarinin (30) and sesamol (31) were generated by oxidative derivation from (+)-sesamolin (2) and (+)-sesamin (3), two abundant lignans found in sesame seeds. To evaluate their in vitro antioxidant potential, the seven isolated lignans (1-7) and the two derivatives (30 and 31) were examined for the scavenging activities on DPPH free radicals and superoxide anions. Moreover, the capability of chelating ferrous ions and reducing power of these sesame lignans were also measured. The results suggest that, besides the well-known sesamolin and sesamin, the minor sesame lignans (+)-(7S,8'R,8R)-acuminatolide (5), (-)-piperitol (6), and (+)-pinoresinol (7) are also adequate active ingredients and may be potential sources for nutritional and pharmacological utilization. 相似文献
2.
Shyu DJ Chou WM Yiu TJ Lin CP Tzen JT 《Journal of agricultural and food chemistry》2004,52(5):1350-1356
A cDNA fragment encoding cystatin, a cysteine protease inhibitor, was obtained from maturing sesame seeds. The clone was constructed in a nonfusion or fusion vector and then overexpressed in Escherichia coli. The recombinant cystatins were found in the soluble fraction of cell extract and were demonstrated to be functionally active in a reverse zymographic assay. The corresponding endogenous 22 kDa cystatin of low abundance in mature seeds was purified to homogeneity via a papain-coupling affinity column and confirmed by western blotting with antibodies against the recombinant cystatin. Both endogenous and recombinant cystatin proteins showed effective inhibitory activities against papain with K(i) values of 7.89 x 10(-8) M and 2.77 x 10(-8) M, respectively. Immunodetection indicated that cystatin was specifically expressed in maturing seeds and rapidly degraded in germination. Accordingly, zymographic and inhibition analyses showed that sesame cystatin could not inhibit the de novo synthesized proteases in germinating seeds. It is suggested that sesame cystatin may play a role in the regulation of endogenous cysteine proteases during seed maturation and germination. 相似文献
3.
Policosanol has no antioxidant activity in human low-density lipoprotein but increases excretion of bile acids in hamsters 总被引：3，自引：0，他引：3
Policosanol is a group of long chain primary alcohols and has been shown to reduce blood cholesterol levels and to inhibit the oxidation of low-density lipoprotein (LDL). The present study examined (i) the effect of policosanol supplementation in the diet on the fecal excretion of neutral and acidic sterols in hamsters and (ii) the antioxidant activity of policosanol in human LDL. Golden Syrian hamsters were divided into four groups (n = 12/each) fed one of the four diets containing 0 (control), 0.38, 0.75, and 1.50 g kg(-1) policosanol for 6 weeks. It was found that hamsters given 0.38-1.5 g kg(-1) diets had a serum total cholesterol level lowered by 15-25% and had a high-density lipoprotein cholesterol elevated by 7-16.8%. It was found that policosanol increased the excretion of acidic sterols by 25-73%. Contrary to that in previous reports, policosanol had no apparent anti-LDL oxidation activity when 1-tetracosanol, 1-hexacosanol, and 1-octacosanol were incubated in human LDL. Policosanol also possessed no scavenging activity on the free radical2,2-diphenyl-1-picrylhydrazyl. These data provide evidence that in addition to the effect of HMG-CoA reductase, the cholesterol-lowering activity of policosanol is partially mediated by its inhibition on the absorption of bile acids, but these data disprove the claim that policosanol is an antioxidant. 相似文献
4.
Identification and characterization of a late gene encoded by grouper iridovirus 2L (GIV‐2L) 
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下载免费PDF全文 Grouper iridovirus (GIV) belongs to the Ranavirus genus and is one of the most important viral pathogens in grouper, particularly at the fry and fingerling stages. In this study, we identified and characterized the GIV‐2L gene, which encodes a protein of unknown function. GIV‐2L is 1242 bp in length, with a predicted protein mass of 46.2 kDa. It displayed significant identity only with members of the Ranavirus and Iridovirus genera. We produced mouse monoclonal antibodies against the GIV‐2L protein by immunizing mice with GIV‐2L‐His‐tag recombinant protein. By inhibiting de novo protein and DNA synthesis in GIV‐infected cells, we showed that GIV‐2L was a late gene during the viral replication. Finally, immunofluorescence microscopy revealed that GIV‐2L protein accumulated in both the nucleus and cytoplasm of infected cells. These results offer important insights into the pathogenesis of GIV. 相似文献
5.
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
Chi-Yao Chang Wei-Jung Chen Jinn-Chin Yiu Su-Der Chen Yu-Shen Lai 《Research in veterinary science》2010,88(2):285-293
Three canine mammary tumor (CMT) cell lines, namely DE-E, DE-F and DE-SF, have been established from a surgically excised specimen of a malignant mammary tumor. These CMT cell lines have been cultured for over 200 passages. The cell doubling time was estimated to be approximately 30 h for all three cell lines. DE-E, DE-F and DE-SF were epithelial, fibroblast and spindle fibroblast in morphology, respectively. Under electron microscope, DE-F and DE-SF cells displayed a higher nucleus/cytoplasm ratio as compared with DE-E. Variation in chromosome number was also observed in the three cell lines. In addition to the morphological characteristics, these cell lines displayed differential patterns of several known mammary tumor cell markers. Following xenotransplantation of the CMT cells into nude mice, DE-F and DE-SF developed tumors within 2 weeks, whereas DE-E failed to develop any visible tumor up to 8 weeks after injection. Lastly, the CMT cell lines exhibited differential chemoresistance to several anti-tumor drugs, including melatonin, cyclosporine A, tamoxifen and indole, suggesting that these cell lines can be used as a comparative experimental model for the tumorigenesis of mammary carcinomas and a valuable tool for anti-cancer drug screening. 相似文献
6.
有机农业在香港幼联园艺农场的实践 总被引：1，自引：0，他引：1
有机农业的研究及推广在香港仅有十余年的历史。实践证明,有机耕种技术在香港幼联园艺农场获得了很大的成功,并引起外国学术界的关注。 相似文献
7.
杂交稻叶面专用新药“稻多收”的增产效果研究 总被引：1，自引：0，他引：1
三年多来,新药"稻多收"已经用于杂交稻生产.本文报道了它的施用方法、增产效果及原因.在一般情况下,只需施药一次,每亩200克,叶面喷施,亩增产稻谷在50公斤以上. 相似文献
8.
Jinn-Chin Yiu Lao-Dar Juang Denise Yi-Tan Fang Cheng-Wei Liu Sheng-Ju Wu 《Scientia Horticulturae》2009
Soil flooding is an environmental factor of seasonal occurrence that negatively affects plant performance. Polyamines play an important role in the plant response to adverse environmental conditions including flooding stress. The objective of the present study was to assess the comparative oxidative damage to Welsh onion plants caused by flooding stress and to examine the role of putrescine (Put) in this response. Welsh onion plants were treated with Put prior to 10 d flooding. A positive effect was observed when treating with 2 mM Put. Exogenous application of Put resulted in alleviation of flooding-induced reduced relative water content, plant growth and chlorophyll fluorescence. Superoxide radical (O2−) and hydrogen peroxide (H2O2) contents were also reduced in stressed plants after Put pre-treatment and thereby the oxidative stress in plant cells was lowered. The antioxidant system, as an important component of the waterlogging-stress-protective mechanism including α,α-diphenyl-β-picrylhydrazyl (DPPH)-radical scavenging activity, superoxide anion radical scavenging, metal chelating activities and reducing power, can be upgraded by Put, which is therefore able to moderate the radical scavenging system and to lessen oxidative stress. Under non-flooding conditions, the anti-oxidative activity of Welsh onion was regulated and elevated by Put pre-treatment. These results suggest that Put confers flooding tolerance to Welsh onion, probably through inducing the activities of various anti-oxidative systems. Thus, exogenous 2 mM per plant of Put 24 h prior to flooding could alleviate flooding stress. 相似文献
9.
Chitosan is a natural polycationic linear polysaccharide derived from chitin. The low solubility of chitosan in neutral and alkaline solution limits its application. Nevertheless, chemical modification into composites or hydrogels brings to it new functional properties for different applications. Chitosans are recognized as versatile biomaterials because of their non-toxicity, low allergenicity, biocompatibility and biodegradability. This review presents the recent research, trends and prospects in chitosan. Some special pharmaceutical and biomedical applications are also highlighted. 相似文献
10.
Peptides are important bioactive natural products which are present in many marine species. These marine peptides have high potential nutraceutical and medicinal values because of their broad spectra of bioactivities. Their antimicrobial, antiviral, antitumor, antioxidative, cardioprotective (antihypertensive, antiatherosclerotic and anticoagulant), immunomodulatory, analgesic, anxiolytic anti-diabetic, appetite suppressing and neuroprotective activities have attracted the attention of the pharmaceutical industry, which attempts to design them for use in the treatment or prevention of various diseases. Some marine peptides or their derivatives have high commercial values and had reached the pharmaceutical and nutraceutical markets. A large number of them are already in different phases of the clinical and preclinical pipeline. This review highlights the recent research in marine peptides and the trends and prospects for the future, with special emphasis on nutraceutical and pharmaceutical development into marketed products. 相似文献