Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage     

Masashi Ando  Masao Joka  Satoshi Mochizuki  Koh-Ichi Satoh  Yasuyuki Tsukamasa  Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751

ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel.  相似文献   

Lens formation in the absence of optic cup in rat embryos irradiated with soft X-ray   总被引：1，自引：0，他引：1  

Inagaki S  Kotani T 《Veterinary ophthalmology》2003,6(1):61-66

In order to investigate the effect of soft X-ray irradiation on ocular development, pregnant rats were exposed to a single 12.5 Gy irradiation on embryonic day 9 (ED 9). The embryos obtained by laparotomy on ED 12 and 21 were examined for ocular abnormalities under a binocular stereo-microscope and a light microscope. The ED 12 embryos were stained with osmium tetroxide to facilitate the observation. The stereo-microscopic examination on ED 12 and 21 revealed various types of ocular abnormalities characterized primarily by aplasia or hypoplasia of the optic cup and invaginated lens placode. The light microscopic examination further confirmed these findings histomorphologically, and the hypoplastic abnormalities were classified into three types: (1) hypoplasia of the optic cup and invaginated lens placode, (2) complete malformation of the optic cup and hypoplasia of the invaginated lens placode, and (3) complete malformation of the optic cup and invaginated lens placode. Because the lens was formed in the complete absence of the retina, the development and differentiation of the retina and lens do not seem to be tightly synchronized. Thus, this sequential analysis on ocular abnormalities during the early stage of development supports the notion that the presence of the retina is not always necessary for the development of the lens.  相似文献   

Identification of Petunia hybrida cultivars that diurnally emit floral fragrances     

Kiriko Nakamura  Kiyoshi Matsubara  Hitoshi Watanabe  Hisashi Kokubun  Yoshihiro Ueda  Naomi Oyama-Okubo  Masayoshi Nakayama  Toshio Ando 《Scientia Horticulturae》2006

In order to identify genetic resources for breeding fragrant petunias for use as bedding plants, volatile compounds released by day from the flowers of 40 commercial Petunia hybrida cultivars were analyzed using a solid-phase micro-extraction technique coupled with GC–MS. The three cultivars with solid deep-blue flowers that accumulate malvidin in corollas with high tissue pH were found to emit abundant iso-eugenol as the principal floral fragrance. Several other cultivars that emitted considerable amounts of methylbenzoate and/or benzylbenzoate from the flower were also identified. Association between the floral fragrance and the other floral traits such as floral anthocyanin composition and corolla-tissue pH was discussed.  相似文献   

Genetic improvement in pine wilt disease resistance inPinus thunbergii: The effectiveness of pre-screening with an artificial inoculation at the nursery     

Tadao Toda  Susumu Kurinobu 《Journal of Forest Research》2001,6(3):197-201

Open pollinated progenies from 15 clones of Japanese black pine (Pinus thunbergii), that had been selected for their tolerance to pine wilt disease, were tested by artificial inoculation with pine wood nematode for six years at a nursery of Kyushu Regional Breeding Office. Family variations in the respective year were all significant. An analysis of variance across the six years’ tests confirmed highly significant effect of year and of families; however, the year by family interaction was significant also. Phenotypic and genetic variances estimated from each year’s result were fitted to a quadratic regression using their mean survival ratio as an independent variable. The variances were greatest at around 0.5 of the mean survival ratio. Estimated heritability showed a similar trend of change, whereas the peak of the expected amount of improvement shifted toward the lower survival range: around 0.4, where a greater selection differential was anticipated. It was concluded that a useful amount of genetic improvement that may be reduced by 30% due to the interaction, would be expected by prescreening seedlings where the mean survival ratio after inoculation was around 0.25 to 0.6.  相似文献   

Suppression of fish meat softening by strict control of storage temperature     

Masashi Ando  Shintaro Mizuochi  Yasuyuki Tsukamasa  Ken -Ichi Kawasaki 《Fisheries Science》2007,73(3):705-712

ABSTRACT:   Changes in meat firmness and structure of cultured yellowtail at several storage temperatures, from −1.5°C to 10°C, were compared. Firmness decreased less at −1.5°C and 10°C than at the other temperatures. During storage at temperatures from −0.5 to 4.0°C, expansion of intercellular space, which indicates weakening of the connecting force between muscle cells, began to increase after 8 h storage, and the ratio of space to the whole area increased by 3.4–4.9 times after 24 h of storage. However, at −1.5°C and 10°C, the increase of intercellular space was suppressed, and the ratio was 2.4–2.6 times, even after 24 h storage. Collagen fibrils connecting muscle cells disintegrated after 24 h storage at all storage temperatures. Conversely, in 10°C storage, the fine structure of muscle, except for collagen fibrils, was maintained better than at other storage temperatures. These findings indicate that −1.5 and 10°C storage could maintain fish-meat quality for longer periods than storage at −0.5, 1.0, or 4.0°C, the normal refrigeration temperature.  相似文献   

Changes in myoglobin content and proximate compositions of the dorsal ordinary muscles of full-cycle cultured Pacific bluefin tuna Thunnus orientalis (Temminck et Schlegel) with body size     

Yasuyuki Tsukamasa  Yoshi-Nori Nakamura  Masashi Ando  Manabu Seoka  & Ken-Ichi Kawasaki 《Aquaculture Research》2007,38(2):201-205

We investigated the changes in myoglobin (Mb) content and proximate compositions of the cephalad parts of the dorsal ordinary muscles (Ce‐DOM) of full‐cycle cultured (FC) Pacific bluefin tuna [body length: 42.6–115.4 cm, body weight (BW): 1.7–38.4 kg, killed from July 2004 to October 2005]. The Mb content of Ce‐DOM increased (P<0.01) until a BW of 7.0 kg was reached. However, the Mb content of Ce‐DOM had a wide variety above a BW of 7.0 kg. On the other hand, the moisture, protein and ash contents of Ce‐DOM of FC tuna decreased (P<0.05 and 0.01) with an increase in BW. However, the lipid content of Ce‐DOM increased gradually above about a BW of 20.0 kg. Furthermore, the Mb content in protein of the Ce‐DOM of FC tuna increased with an increase in BW. These results indicated that the increase in Mb content of Ce‐DOM of FC tuna was not related to changes in proximate composition between a BW of 1.7 and 38.4 kg.  相似文献   

The effectiveness of a new physical property evaluation method of kamaboko which applied the principal component analysis to the puncture and stress relaxation tests     

Tomokazu Hagihara  Masashi Ando  Kenichi Kawasaki  Yasuo Makinodan  Yasuyuki Tsukamasa 《Fisheries Science》2006,72(2):393-401

ABSTRACT:   The physical properties of 49 commercial kamabokos were measured by the puncture and stress relaxation tests. The principal component analysis was applied to the physical parameters of both tests, and their cumulative contribution ratios were over 90% with the first and second principal components, respectively. The comparison among the kamabokos was carried out using the synthetic physical parameters. The kamabokos produced in same area showed the peculiar distribution. The relative positional relation of kamabokos measured by the stress relaxation test was different from that by the puncture test for many of the kamabokos. Physical property evaluation using a principal component analysis is very effective for intensiveness of many measurement parameters. If much more kamabokos were measured by this method, regional characteristics of kamabokos would be clarified.  相似文献   

The changes in proximate compositions and glycogen contents in the dorsal ordinary muscles of the full-cycle cultured Pacific bluefin tuna Thunnus orientalis occurring with growth     

Yoshi -Nori Nakamura  Masashi Ando  Manabu Seoka  Ken -Ichi Kawasaki  Yasuyuki Tsukamasa 《Fisheries Science》2006,72(6):1140-1146

ABSTRACT:   Using full-cycle cultured (FC) Pacific bluefin tuna (body length [BL], 42.6–66.4 cm; body weight [BW], 1.66–7.40 kg, n  = 15), the changes in chemical compositions and histological structure of the cephalal parts of the dorsal ordinary muscles (DOM) occurring with growth were investigated. A positive correlation ( r  = 0.9644, P  < 0.05) was observed between BL and BW with growth. The protein, lipid and ash contents of DOM and condition factor did not change with growth. However, the glycogen content of DOM increased ( P  < 0.05) from approximately 55 cm (BL) in this study. Using optical microscopic photographs, the various shapes of muscle fibers were observed and it was noted that the muscle fiber diameter increased ( P  < 0.05) with growth. Using transmission electron microscopic observation, many glycogen granules were observed in muscle fibers (especially, side of connective tissue) of DOM throughout the growth stage in this study. These results indicate that the glycogen content of DOM of FC Pacific bluefin tuna increases before the lipid with growth.  相似文献   

Chlorantraniliprole application differentially affects adult emergence of Sympetrum dragonflies in rice paddy fields     

Nakanishi  Kosuke  Usio  Nisikawa  Yokomizo  Hiroyuki  Takashima  Tadao  Hayashi  Takehiko I. 《Paddy and Water Environment》2022,20(1):177-183

Paddy and Water Environment - Rice paddy fields are important habitat for many dragonfly species. In Japan, populations of dragonflies inhabiting rice paddies, in particular Sympetrum (Odonata:...  相似文献   

Isolation of purple nor-carotenoid, 2′, 3′-dihydroroserythrin,from crawfish <Emphasis Type="Italic">Procambarus clarkii</Emphasis>     

Takashi Maoka  Seiichi Ando 《Fisheries Science》2007,73(4):967-968

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