Assessment of pulse co-oximetry technology after in vivo adjustment in anaesthetized dogs     

Hamaseh Tayari  Derek Flaherty  Tyfane T. Yamaoka  Adam Auckburally 《Veterinary anaesthesia and analgesia》2021,48(3):297-304

ObjectiveTo compare values of haemoglobin concentration (SpHb), arterial haemoglobin saturation (SpO₂) and calculated arterial oxygen content (SpOC), measured noninvasively with a pulse co-oximeter before and after in vivo adjustment (via calibration of the device using a measured haemoglobin concentration) with those measured invasively using a spectrophotometric-based blood gas analyser in anaesthetized dogs.Study designProspective observational clinical study.AnimalsA group of 39 adult dogs.MethodsIn all dogs after standard instrumentation, the dorsal metatarsal artery was catheterised for blood sampling, and a pulse co-oximeter probe was applied to the tongue for noninvasive measurements. Paired data for SpHb, SpO₂ and SpOC from the pulse co-oximeter and haemoglobin arterial oxygen saturation (SaO₂) and arterial oxygen content (CaO₂) from the blood gas analyser were obtained before and after in vivo adjustment. Bland–Altman analysis for repeated measurements was used to evaluate the bias, precision and agreement between the pulse co-oximeter and the blood gas analyser. Data are presented as mean differences and 95% limits of agreement (LoA).ResultsA total of 39 data pairs were obtained before in vivo adjustment. The mean invasively measured haemoglobin–SpHb difference was –2.7 g dL^?1 with LoA of –4.9 to –0.5 g dL^?1. After in vivo adjustment, 104 data pairs were obtained. The mean invasively measured haemoglobin–SpHb difference was –0.2 g dL^?1 with LoA of –1.1 to 0.6 g dL^?1. The mean SaO₂–SpO₂ difference was 0.86% with LoA of –0.8% to 2.5% and that between CaO₂–SpOC was 0.66 mL dL^–1 with LoA of –2.59 to 3.91 mL dL^–1.ConclusionsBefore in vivo adjustment, pulse co-oximeter derived values overestimated the spectrophotometric-based blood gas analyser haemoglobin and CaO₂ values. After in vivo adjustment, the accuracy, precision and LoA markedly improved. Therefore, in vivo adjustment is recommended when using this device to monitor SpHb in anaesthetised dogs.  相似文献   

The role of primary germ tubes in the life cycle of Blumeria graminis: The primary germ tube is responsible for the suppression of resistance induction of a host plant cell     

Naoto Yamaoka  Takayuki Ohta  Naoko Danno  Satoshi Taniguchi  Isao Matsumoto  Masamichi Nishiguchi 《Physiological and Molecular Plant Pathology》2007,71(4-6):184-191

When the conidia of Blumeria graminis f. sp. hordei (Bgh) are inoculated on barley coleoptile cells they produce short germ tubes called primary germ tubes (PGTs) about 2 h after inoculation. We evaluated the positive role of a PGT in inducing accessibility of the host cell under the germ tube. When an appressorium (APP) penetrated the same cell on which a PGT was present, the ratio of haustorium formation (penetration efficiency) was significantly higher than when an APP penetrated the cell adjacent to the one on which a PGT was present. When an APP penetrated the cell laterally adjacent to the one on which a PGT was present we killed the cell under the PGT by puncturing it with a microneedle and then investigated the penetration efficiency of the cell adjacent to the dead cell. As a control we killed the cell longitudinally adjacent to the one on which a PGT was present and investigated the penetration efficiency of the laterally adjacent cell. The results showed that the penetration efficiency of the former was significantly lower than that of the latter. This suggests that some accessibility factor might transfer from a cell on which a PGT is present to a laterally adjacent cell. The existence of a conidium body but not a PGT was not effective for induced accessibility of the host cell. Moreover, when a Bgh germling was removed 6 h after inoculation and another germling was transferred to the same cell, the penetration efficiency was significantly higher than that of control. As a control, a Bgh germling was transferred to a cell on which no germling was present. These results suggest that the existence of PGT is effective for induced accessibility of a host cell when penetrated by Bgh. However, it is unclear whether or not a PGT secretes some substance(s) which suppresses the resistance induction of a host cell.  相似文献   

Comparative virulence of blue-stain fungi isolated from Japanese red pine   总被引：2，自引：0，他引：2  

H. Masuya  S. Kaneko  Y. Yamaoka 《Journal of Forest Research》2003,8(2):0083-0088

 We made inoculations to determine the comparative virulence of blue-stain fungi isolated from Japanese red pine (Pinus densiflora Sieb. & Zucc.). Based on the symptoms caused by each fungus, the most virulent fungus was Leptographium wingfieldii. Also, Ophiostoma minus was relatively virulent while lesions induced by some species did not differ from those in the control treatment. Based on the results, we concluded that the pathogenicity of the blue-stain fungi varied widely, and thus their impact on the host most likely varies too. Received: June 7, 2002 / Accepted: October 4, 2002 Present address: JST Domestic Research Fellow, Forest Pathology Laboratory, Tohoku Research Center, Forestry and Forest Products Research Institute, 92-25 Nabeyashiki, Shimo-Kuriyagawa, Morioka 020-0123, Japan Tel +81-19-648-3961; Fax +81-19-641-6747 e-mail: H_masu@hotmail.com Acknowledgments We thank Drs. Ohtaka and Usuki, Plant Pathology and Mycology Laboratory, Institute of Agriculture and Forestry, University of Tsukuba, for technical assistance. Contribution No. 174, Plant Pathology and Mycology Laboratory, Institute of Agriculture and Forestry, University of Tsukuba. Correspondence to:H. Masuya  相似文献   

Ophiostomatoid fungi associated with bark beetles onAbies veitchii in wave-regenerated forests     

Nobuaki Ohtaka  Hayato Masuya  Shigeru Kaneko  Yuichi Yamaoka  Masashi Ohsawa 《Journal of Forest Research》2002,7(3):145-151

Relationships between tree mortality and bark beetle infestation onAbies veitchii at the wave-regenerated forest in Mt. Asahi, Okuchichibu area were investigated. Most of the firs with green needles and newly developed current year’s shoots in the dieback zone were heavily infested by bark beetles before the death of the trees. After heavy infestation of beetles, about half of the infested firs died within the year, and the other half died in the next year. When the species composition of bark beetles and associated ophiostomatoid fungi were investigated in Mt. Asahi and also at a typical wave-regenerated forest in Mt. Shimagare, Yatsugatake area,Cryphalus montanus andC. piceae were dominant beetle species for Mt. Asahi and Mt. Shimagare, respectively.Ophiostoma subalpinum andO. europhioides were dominant fungal species at both wave-generated forests. BecauseO. subalpinum was detected more frequently from deeper areas of sapwood thanO. europhioides, it was suggested that the fungal species may accelerate the death of stressed firs in wave-regenerated forests. Contribution No. 169, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba.  相似文献   

Anaemia, iron deficiency and vitamin A status among school-aged children in rural Kazakhstan     

Hashizume M  Chiba M  Shinohara A  Iwabuchi S  Sasaki S  Shimoda T  Kunii O  Caypil W  Dauletbaev D  Alnazarova A 《Public health nutrition》2005,8(6):564-571

OBJECTIVES: To investigate the prevalence of anaemia and iron deficiency and vitamin A status among school-aged children in rural Kazakhstan and identify factors associated with anaemia in this population. DESIGN: A cross-sectional design. SETTING: School-aged children in rural Kazakhstan. SUBJECTS: Socio-economic and anthropometric information was collected from 159 school-aged children living in the Kzyl-Orda region of Kazakhstan. Blood samples were collected and the concentrations of haemoglobin (Hb), serum iron, serum ferritin (SF), erythrocyte protoporphyrin (EP), serum retinol and beta-carotene, total iron binding capacity (TIBC), transferrin saturation (TS) and other haematological indices were measured. RESULTS: Among the 159 children, the prevalence of anaemia and iron deficiency defined by the multiple criteria model (SF, TS and EP) was 27% and 13%, respectively. Nine per cent had iron-deficiency anaemia and 21% had serum retinol value < 1.05 micromol l(-1). Mean SF and serum iron concentrations and TS were significantly lower in anaemic children than in their non-anaemic peers, while TIBC and EP were significantly higher in children with anaemia. Hb was significantly correlated with serum iron and retinol concentrations. Serum retinol and SF concentrations and mean corpuscular volume were significantly correlated with Hb by multiple regression analysis. CONCLUSIONS: Anaemia among school-aged children in rural Kazakhstan appears to be related to iron indices and vitamin A status.  相似文献   

Traces of human migrations in Helicobacter pylori populations     

Falush D  Wirth T  Linz B  Pritchard JK  Stephens M  Kidd M  Blaser MJ  Graham DY  Vacher S  Perez-Perez GI  Yamaoka Y  Mégraud F  Otto K  Reichard U  Katzowitsch E  Wang X  Achtman M  Suerbaum S 《Science (New York, N.Y.)》2003,299(5612):1582-1585

Helicobacter pylori, a chronic gastric pathogen of human beings, can be divided into seven populations and subpopulations with distinct geographical distributions. These modern populations derive their gene pools from ancestral populations that arose in Africa, Central Asia, and East Asia. Subsequent spread can be attributed to human migratory fluxes such as the prehistoric colonization of Polynesia and the Americas, the neolithic introduction of farming to Europe, the Bantu expansion within Africa, and the slave trade.  相似文献   

A new probe for the diagnosis of myotonic muscular dystrophy   总被引：11，自引：0，他引：11  

R J Bartlett  M A Pericak-Vance  L Yamaoka  J Gilbert  M Herbstreith  W Y Hung  J E Lee  T Mohandas  G Bruns  C Laberge 《Science (New York, N.Y.)》1987,235(4796):1648-1650

Myotonic muscular dystrophy (DM) is the most common muscular dystrophy, affecting adults as well as children. It is inherited as an autosomal dominant trait and is characterized by variable expressivity and late age-of-onset. Linkage studies have established the locus on chromosome 19. In order to identify tightly linked probes for diagnosis as well as to define in detail the DM gene region, chromosome 19 libraries were constructed and screened for restriction fragment length polymorphisms tightly linked to DM. A genomic clone, LDR152 (D19S19), was isolated that is tightly linked to DM; recombination fraction = 0.0 (95% confidence limits 0.0-0.03); lod score, 15.4.  相似文献   

Growth, Net Photosynthetic Rate, Nutrient Status and Secondary Xylem Anatomical Characteristics of Fagus Crenata Seedlings Grown in Brown Forest Soil Acidified with H2SO4 Solution     

Takeshi Izuta  Taeko Yamaoka  Tatsuro Nakaji  Tetsushi Yonekura  Masaaki Yokoyama  Hideyuki Matsumura  Sachie Ishida  Kenichi Yazaki  Ryo Funada  Takayoshi Koike 《Water, air, and soil pollution》2001,130(1-4):1007-1012

Dry matter production, net photosynthetic rate, leaf nutrient status and trunk anatomical characteristics of Fagus crenata seedlings grown in brown forest soil acidified by adding H₂SO₄ solution were investigated. The soil acidification leaded to decreased (Ca+Mg+K)/Al molar ratio in the soil solution. Dry mass per plant of the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly reduced compared with the control value at 0 mg·L^?1. When net photosynthetic rate was reduced in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1, the carboxylation efficiency and maximum net photosynthetic rate at saturated CO₂-concentration were lower than the control values. The addition of H⁺ to the soil at 120 mg·L^?1iinduced a reduction in the concentration of Ca in the leaf. By contrast, the concentration of Al in the leaf was increased with increasing the amount of H⁺ added to the soil. The annual ring formed in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly narrower than that at 0 (control), 10, 30, 60 or 90 mg·L^?1. Based on the results obtained in the present study, we conclude that Fagus crenata is relatively sensitive to a reduction in the (Ca+Mg+K)/Al molar ratio of soil solution compared with Picea abies.  相似文献   

Multitracer Study on Absorption of Radionuclides in Atmosphere-Plant Model System     

Taeko Shinonaga  Shizuko Ambe 《Water, air, and soil pollution》1998,101(1-4):93-103

The absorption of atmospheric radionuclides by soybean plants was experimentally studied using a radioactive multitracer consisting of radionuclides of Sc, Mn, Co, As, Se, Rb, Sr, Y, Eu, Gd, Yb, Hf, Re, and Ir. The soybean plants were cultivated in soil under no-rain conditions in a box containing air in which multitracer-absorbed cellulose particles were dispersed. The soil was covered with plastic film. After about one-month cultivation, the plants were harvested and washed with dilute HCl solution. The leaves, stems, and beans were subjected to γ-ray measurement. The radionuclides of As, Se, and Re were accumulated in the leaves, and those of Co, Se, and Rb were accumulated in the beans. Among them Se was accumulated to a large extent in the leaves and in the beans. Small amounts of the radionuclides of Co, Se, Rb and Re were found in the roots. Little absorption of these radionuclides into the soil occurred. These results indicate that various radionuclides in the atmosphere were absorbed through the soybean leaves and then transported to the seeds. These findings must be taken into consideration for a complete understanding of the system of uptake of radionuclides by the soybean plant.  相似文献   

Rice necrosis mosaic virus,a fungal transmitted Bymovirus: complete nucleotide sequence of the genomic RNAs and subgrouping of bymoviruses     

S. G. Wagh  K. Kobayashi  T. Yaeno  N. Yamaoka  C. Masuta  M. Nishiguchi 《Journal of General Plant Pathology》2016,82(1):38-42

  相似文献