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1.
Hojjat-Allah Abbaszadeh Taki Tiraihi Ali Reza Delshad Majid Saghedi Zadeh Taher Taheri 《Iranian Biomedical Journal》2013,17(2):62-70
Background: The present study investigated the functional maturity of oligodendrocyte derived from rat bone marrow stromal cells (BMSC). Methods: The BMSC were isolated from female Sprague-Dawley rats and evaluated for different markers, such as fibronectin, CD106, CD90, Oct-4 and CD45. Transdifferentiation of OLC from BMSC was obtained by exposing the BMSC to DMSO and 1 µM all-trans-retinoic acid during the pre-induction stage and then induced by heregulin (HRG), platelet-derived growth factor AA (PDGFR-α), fibroblast growth factor and T3. The neuroprogenitor cells (NPC) were evaluated for nestin, neurofilament 68, neurofilament 160 and glial fibrillary acidic protein gene expression using immunocytochemistry. The OLC were assessed by immunocytochemistry for O4, oligo2, O1 and MBP marker and gene expression of PDGFR-α was examined by RT-PCR. Results: Our results showed that the fibronectin, CD106, CD90, CD45 and Oct-4 were expressed after the fourth passage. Also, the yield of OLC differentiation was about 71% when using the O1, O4 and oligo2 markers. Likewise, the expression of PDGFR-α in pre-oligodendrocytes was noticed, while MBP expression was detected in oligodendrocyte after 6 days of the induction. Conclusion: The conclusion of the study showed that BMSC can be induced to transdifferentiate into mature OLC. Key Words: Bone marrow stromal cell, Triiodothyronine, Platelet-derived growth factor α 相似文献
2.
Taher Mechergui Marta Pardos Naceur Boussaidi Brahim Hasnaoui Douglass F. Jacobs 《林业研究》2013,24(2):193-204
The need for reforestation in cork oak (Quercus suber L.) areas is challenged by difficulties. Principal among these is herbivory of young plants, vegetative competition, and slow growth rates of cork oak seedlings. We evaluated the early development of cork oak seedlings treated using tree shelters and mulching in northwestern Tunisia. We tested three tree shelter treatments (non-vented, vented, and control) to shield seedlings from animal damage and five mulch types to control competing vegetation (Italian Stone Pine, Lentisk, combination of Italian Stone Pine and Lentisk (organic mulches), gravel (inorganic mulch) and no mulch). At the end of the two-year experiment, sheltered seedlings were 89-99% taller than unshelteredseedlings and had higher numbers and lengths of shoot growth flushes. In contrast, both stem diameter growth and dry weight biomass (from samples extracted after two years) were significantly reduced inside tree shelters. Root-to-shoot ratio was not significantly different in sheltered vs. unsheltered seedlings, suggesting that tree shelters do not adversely affect this parameter. Mulching alone did not favour growth, but could be beneficial when combined with tree shelters. The combination of vented tree shelters and gravel mulch was the most effective treatment for promoting diameter, height and stem volume growth. 相似文献
3.
Muh. Ade Artasasta Yanwirasti Yanwirasti Muhammad Taher Akmal Djamaan Ni Putu Ariantari Ru Angelie Edrada-Ebel Dian Handayani 《Marine drugs》2021,19(11)
Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. Aspergillus nomius NC06 was isolated from the marine sponge Neopetrosia chaliniformis. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (1, 2, and 3), and one known compound, aspergillicin A (4). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds 1, 2, and 4 exhibited IC50 values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds 1, 2, and 4. The result shows that compounds 1 and 2 were able to induce apoptosis and cell death in HT 29 cells. 相似文献
4.
Yield, water and nitrogen-use response of rice to zeolite and nitrogen fertilization in a semi-arid environment 总被引：1，自引：0，他引：1
Water scarcity and soil nitrogen (N) loss are important limitations for agricultural production in semi-arid region especially for rice production. Zeolite (Z) as a soil conditioner can be used to retrain water and nitrogen in near-surface soil layer in lowland rice production system. The objectives of this study were to investigate the effects of different application rates of natural zeolite (clinoptilolite) and nitrogen on rice yield, yield components, soil nitrogen, water use, water productivity in a silty clay soil in 2004 and 2005. Zeolite was only applied in the first year. In order to study the long-term and continuous effect of zeolite on the objectives of the study, no zeolite was applied in the second year and the study was conducted on the same land as the first year. Zeolite and N were applied at rates of 0, 2, 4, and 8 t ha−1 and 0, 20, 40, and 80 kg ha−1, respectively in 2004. In 2005, each plot received the same amount of N as received in 2004. It is concluded that by decreasing N application rates, higher Z application rate is needed to improve grain yield. Highest grain yield was obtained at N application rate of 80 kg ha−1 and Z application rate of 4 t ha−1. Higher grain yield was mostly attributed to lower unfilled grain percentage and higher 1000-grain weight that were a result of higher N application rate and N retention in soil due to Z application. Nitrogen and Z applications resulted in higher grain protein contents and nitrogen recovery efficiency (NRE). Based on these results and due to higher N retention in soil under Z application, improved grain yield quality, nitrogen-use efficiency (NUE), and nitrogen recovery efficiency (NRE) could be obtained at Z application rate of 8 t ha−1 and N application rate of 80 kg ha−1 or more. However, this was not satisfied for NUE. Moreover, it is found that at higher N application rates lower Z application rates are needed to effectively retain soil residual mineral nitrogen. Furthermore, at N application rates of 80 kg ha−1 or more, Z application increased soil water retention and resulted in lower seasonal water use and higher water productivity. In general, it was concluded that the effect of Z application in retaining soil N was also effective in the second year. 相似文献
5.
M Koushesh Saba K Arzani M Barzegar 《Journal of agricultural and food chemistry》2012,60(36):8947-8953
Fruit of two apricot cultivars 'Bagheri' and 'Asgarabadi' were treated with putrescine (Put) or spermidine (Spd) at 1 mM and then were stored at 1 °C for 21 days. Fruit were sampled weekly and stored 2 days at 20 °C for shelf-life study. The treatments reduced ethylene production and maintained the firmness and color of the fruit. Peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), and polyphenol oxidase (PPO) activities and total phenol (TP) concentrations were measured during storage. Both cultivars showed chilling injury (CI) incidence, and the severity in control fruit was higher than either Put or Spd treatments. CI incidence in Spd-treated fruit was lower than that of Put-treated fruit. Polyamine (PA) treatment generally increased antioxidant enzyme activity of fruit during storage. PA treatments may help maintain the quality of apricot fruit during storage by inhibiting ripening and decreasing CI incidence. 相似文献
6.
Laroucau K de Barbeyrac B Vorimore F Clerc M Bertin C Harkinezhad T Verminnen K Obeniche F Capek I Bébéar C Durand B Zanella G Vanrompay D Garin-Bastuji B Sachse K 《Veterinary microbiology》2009,135(1-2):82-89
Five severe cases of psittacosis in individuals associated with duck farms were notified in France between January and March 2006. Diagnostic examination included serology and/or molecular detection by PCR from respiratory samples. As a consequence, we investigated all duck flocks (n=11) that were housed in the three farms where human infections occurred. While serology by complement fixation test was negative for all samples, cloacal and/or tracheal chlamydial excretion was detected by PCR in all three units. Notably, one duck flock was tested strongly positive in 2 of the 3 affected farms, and Chlamydophila (C.) psittaci strains were isolated from cloacal and/or tracheal swab samples from both farms. Human samples and duck isolates exhibited the same PCR-RFLP restriction pattern, which appeared to be an intermediate between genotypes A and B. Analysis of ompA gene sequences and comparison to those of the type strains showed that the isolates could not be strictly assigned to any of the generally accepted genotypes of C. psittaci. Further analysis by MLVA of the PCR-positive human samples revealed two distinct patterns, which were related to previously isolated C. psittaci duck strains. 相似文献
7.
Naghizadeh B Boroushaki MT Vahdati Mashhadian N Mansouri MT 《Iranian Biomedical Journal》2008,12(2):93-100
Background. The major side effect of cisplatin, used in some tumours, is nephrotoxicity. Reactive oxygen species and oxidative damage are the most important factors in cisplatin-induced acute renal failure. The main purpose of this study is to investigate the protective effects of crocin against cisplatin-induced acute renal failure and oxidative stress in rat. Methods. In this study, animals were randomly divided into 5 groups (6 each). Group one received normal saline (2 ml/day, i.p.). Group two received a single dose of cisplatin (5 mg/kg, i.p.). Groups 3 to 5 received crocin (100, 200 and 400 mg/kg, i.p., respectively, for 4 consecutive days one hour before a single dose of cisplatin (5 mg/kg) only at the first day. Blood samples were taken out (on the fifth day) for measuring the level of urea and creatinine. The kidneys were removed for histopathological and biochemical examinations. Furthermore, 24-hour urinary factors were measured. Results. Blood urea, creatinine and urinary glucose and protein concentrations in crocin-treated groups were significantly lower than those of cisplatin-treated group in a dose-dependent manner. Histopathological studies showed a massive damage in S3 segment of proximal tubules in cisplatin-treated group. No damage was observed in crocin-treated groups. Crocin treatment resulted in a significant and dose-dependent reduction in malondialdehyde concentration as compared to the cisplatin-treated group. Moreover, crocin produced a significant elevation in total thiol and glutathione peroxidase concentrations, as compared with cisplatin-treated group. Conclusion. The results of the present study suggest that crocin has a protective effect against cisplatin-induced acute renal failure and relative oxidative stress. 相似文献
8.
Farmer participation in irrigation management: The case of Doroodzan Dam Irrigation Network, Iran 总被引：1，自引：0，他引：1
The importance of farmer participation in system design and management has been emphasized in previous studies. The purpose of this study was to identify the factors affecting farmer participation in irrigation management using survey research. The study was conducted in Doroodzan Dam Irrigation Network in Fars province, Iran. Multistage stratified random sampling was used to collect data from 270 farmers as the research sample. Results reveal that farmers’ attitudes toward participation in irrigation management, attitudes toward personnel of the State Water Authority and the Agricultural Extension Service Centers (AESCs), family size, the problem perception, dependence on the dam for water, and educational background have influenced their participation in irrigation management. By contrast, contact with information sources, animal units, sociability, age and agricultural experience did not affect farmers’ participation. Moreover, based on farmers’ perspectives, unequal water distribution among farms, dissatisfaction with Water Authority operators and high water fees and charges were the main problems and obstacles toward farmer participation in irrigation management. 相似文献
9.
Sraïri MT Kiade N Lyoubi R Messad S Faye B 《Tropical animal health and production》2009,41(5):835-843
Multivariate analyses were used to compare dairy production practices and their consequences on milk yield and profitability
in cattle farms from two representative regions of Morocco. A regular follow-up of 118 farms (48 in the Rabat-Salé suburban
belt and 70 in the Gharb irrigated perimeter) was undertaken to obtain accurate data. Results show significant differences
between the two regions. Intensive milk production was more frequent in the suburban zone (more concentrates and better annual
milk yield per cow). When conducting a “within-region” principal components analysis, farms’ discrimination appeared to take
into account all management variables (feeding, cattle sales, profitability), with no reference to farms’ structural parameters
(arable land and number of cattle). A typology of farms was then established using cluster analysis, with 4 distinct groups,
namely: a) concentrates wasters, b) farms with a relatively important milk yield per cow, c) deficit dairy farms and d) beef oriented farms. The last group included almost exclusively farms from the irrigated perimeter (5 out of 7). These results
indicate that dairy production promotion in Morocco requires more than just the intensification of forage production, but
should focus also on improving management practices. The extension of complete and balanced dairy rations is urgently needed
to enhance milk yield and profitability. 相似文献
10.
A laboratory study was conducted in order to explain successive extractions of reserved potassium by using concentrated HCI under reflux for 13 surface and sub-surface soil samples representing three chosen pedons of soil series （472 ECC Bashika, 463 EKC Rabeaa and 461 ECC Talafar） locations of Nineveh Province in Northern of Iraq. The aim is to investigate role of clay minerals on release of reserved potassium from soils. Results showed that dominated clay minerals were （smectite 〉 illite 〉 kaolinite 〉 chlorite） for both 472 ECC and 461 ECC soil series and were （illite 〉 smectite 〉 kaolinite 〉 chlorite） for 463 EKC soil series. Also results appeared that exchangeable K＋ released values were 2,483-4,575 mg/kg at 461 ECC and 463 EKC soil series, respectively; non-exchangeable phase ranged from 752-1,390 mg/kg at 461 ECC and 472 ECC soil series, respectively. Soil fertility was evaluated according to its K＋ mica release referring for high K＋ release content with range between 3,324-5,516 mg/kg at 461 ECC and 463 EKC soil series, but rate ofK~ released was very law according to parabolic diffusion me,del with range from 195-359 mg/kg at latter soil series respectively. Results reflect effect of clay minerals on potassium release as amount and rates in arid and semi-arid regions. It can be concluded the study soils have a high amount of potassium content with low rate release of it, in turn these soil need particular management. 相似文献