Estrone sulfate and progesterone profiles during late gestation in recipient cows transferred embryos produced by nuclear transfer and in vitro fertilization     

Shah KD  Maeda T  Hidaka T  Ogata Y 《The Journal of reproduction and development》2007,53(6):1237-1246

The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E(1)S) and progesterone (P(4)) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E(1)S and P(4) by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E(1)S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E(1)S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E(1)S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (P<0.05) P(4) concentrations than the average. These results raise the possibility that the E(1)S and P(4) profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation.  相似文献   

Pelvic fin removal modifies escape trajectory in a teleost fish     

Yuuki Kawabata  Hideaki Yamada  Taku Sato  Masato Kobayashi  Koichi Okuzawa  Kimio Asami 《Fisheries Science》2016,82(1):85-93

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Susceptibility of Commercial Tomato Cultivars to Bacterial Wilt in Hydroponic System     

Teruo NONOMURA  Yoshinori MATSUDA  Motoaki TSUDA  Kazuto URANAKA  Hideyoshi TOYODA 《Journal of General Plant Pathology》2001,67(3):224-227

Commercially available tomato cultivars were hydroponically cultured for inoculation, with Ralstonia solanacearum (K-101), which causes bacterial wilt, by pouring an inoculum suspension into the nutrient solution. Cultivar susceptibility to the bacteria was evaluated, based on the highest percentage of wilting. Because the length of time for wilt appearance varied among cultivars, some cultivars appeared to be suppressive to the translocation and/or multiplication of the invading pathogen. Thus, this hydroponic inoculation system is effective for examining levels of susceptibility in tomato cultivars to bacterial wilt. Received 13 December 2000/ Accepted in revised form 27 March 2001  相似文献   

An epidemiological survey of Chrysanthemum chlorotic mottle viroid in Akita Prefecture as a model region in Japan     

Hideki Yamamoto  Teruo Sano 《Journal of General Plant Pathology》2006,72(6):387-390

In epidemiological surveys on Chrysanthemum chlorotic mottle viroid (CChMVd) in various chrysanthemums cultivated in Akita Prefecture, Japan, in 2002–2005, approximately 20% of cultivars harbored CChMVd, including more symptomatic types than nonsymptomatic. Large-flowered cultivars were less frequently infected than small-flowered and spray types. The number of CChMVd-infected chrysanthemums is increasing, and the disease is found throughout major chrysanthemum-producing districts. Chrysanthemums infected only with CChMVd, in general, had no noticeable symptoms of disease. Most of those dually infected with known viruses and/or viroid also had no symptoms characteristic of chlorotic mottle disease. The lack of noticeable symptoms in major Japanese cultivars may have resulted in the unnoticed spread of the viroid.  相似文献   

Post-release movement and diel activity patterns of hatchery-reared and wild black-spot tuskfish <Emphasis Type="Italic">Choerodon schoenleinii</Emphasis> determined by ultrasonic telemetry     

Yuuki Kawabata  Junichi Okuyama  Hiromichi Mitamura  Kimio Asami  Kenzo Yoseda  Nobuaki Arai 《Fisheries Science》2007,73(5):1147-1154

ABSTRACT:   Post-release movement and diel activity patterns of hatchery-reared and wild black-spot tuskfish were examined using ultrasonic telemetry. Five hatchery-reared and four wild fish were released in the sandy bottom of Urasoko Bay, Ishigaki Island, Okinawa, Japan, and monitored using automated monitoring receivers from November 2005 to February 2006. Both hatchery-reared and wild fish tended to stay near the release site for over two weeks, before leaving the release site. Both hatchery-reared and wild tuskfish showed diurnal rhythm intermittently; signals were recorded more frequently in the daytime and less frequently in the nighttime, suggesting that the fish of both origins were active during the day and inactive during the night. These findings indicate that the one-year-old hatchery-reared tuskfish have some consistent behavioral characteristics with those of the wild.  相似文献   

Comparative external morphology of cetacean spermatozoa   总被引：2，自引：0，他引：2  

Shoichi Kita  Motoi Yoshioka  Masaaki Kashiwagi  Satoru Ogawa  Teruo Tobayama 《Fisheries Science》2001,67(3):482-492

SUMMARY: To compare size and morphology of spermatozoa in cetaceans, sperm and epididymis samples were collected from 10 species in four families and spermatozoa were observed with phase-contrast and scanning electron microscopes. According to the average total length of the spermatozoa, the 10 species examined were classified into the following four groups in order of increasing size: (i) Baird's beaked (Ziphiidae) and Bryde's whales (Balaenopteridae); (ii) Dall's and finless porpoises (Phocoenidae); (iii) common, bottlenose, and Pacific white-sided dolphins (Delphinidae); and (iv) killer and short-finned pilot whales, and Risso's dolphin (Delphinidae). Spermatozoa head length of Bryde's whale and the finless porpoise were shorter than those of the other species. Spermatozoa head width was widest in the killer whale and thinnest in the Baird's beaked whale. The lateral aspects of sperm heads from the 10 species were characterized as the 'anterior region of the sperm head is thin and flat while the posterior region is thick.' The dorsal aspects of sperm heads were 'paddle-shaped' in Bryde's whales, 'bowling pin-shaped' in Baird's beaked whales, 'Japanese fan-shaped' in killer whales, an 'elongated ellipsoid shape' in Delphinidae except for killer whales, and 'ellipsoid shaped' in Phocoenidae. Size and morphology of the spermatozoa showed interspecific differences among the 10 species examined, which correspond to cetacean taxonomic classification.  相似文献   

Coexistence of anadromous and lacustrine life histories of the shirauo, Salangichthys microdon   总被引：1，自引：0，他引：1  

T. Arai  H. Hayano  H. Asami  N. Miyazaki 《Fisheries Oceanography》2003,12(2):134-139

The environmental history of the shirauo, Salangichthys microdon, was examined in terms of strontium (Sr) and calcium (Ca) uptake in the otolith, by means of wavelength dispersive X‐ray spectrometry on an electron microprobe. Anadromous and lacustrine type of the shirauo were found to occur sympatric. Otolith Sr concentration or Sr : Ca ratios of anadromous shirauo fluctuated strongly along the life‐history transect in accordance with the migration (habitat) pattern from sea to freshwater. In contrast, the Sr concentration or the Sr : Ca ratios of lacustrine shirauo remained at consistently low levels throughout the otolith. The higher ratios in anadromous shirauo, in the otolith region from the core to 90–230 μm, corresponded to the initial sea‐going period, probably reflecting the ambient salinity or the seawater–freshwater gradient in Sr concentration. The findings clearly indicated that otolith Sr : Ca ratios reflected individual life histories, enabling these anadromous shirauo to be distinguished from lacustrine shirauo.  相似文献   

Mung bean trypsin inhibitor is effective in suppressing the degradation of myofibrillar proteins in the skeletal muscle of blue scad (Decapterus maruadsi)     

Sun LC  Yoshida A  Cai QF  Liu GM  Weng L  Tachibana K  Su WJ  Cao MJ 《Journal of agricultural and food chemistry》2010,58(24):12986-12992

Mung bean trypsin inhibitor (MBTI) of the Bowman-Birk family was purified to homogeneity with a molecular mass of approximately 9 kDa on tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 8887.25 Da as determined by matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight mass spectrometry (MALDI-QIT-TOF MS). Using blue scad myofibrillar proteins as targets, it was found that, in the absence of MBTI, proteolysis of myofibrillar proteins, especially myosin heavy chain (MHC), could be identified after incubation at 55 °C for 2 h, while in the presence of MBTI, with a final concentration of 25 ng/mL, proteolysis of these proteins was greatly suppressed even after incubation for 3 h. Although cysteine proteinase inhibitor E-64 was also effective in preventing protein degradation, inhibitors for metallo- and asparatic proteinases did not reveal obvious inhibitory effects. Our present results strongly suggested that the naturally occurring legume bean seed protein MBTI can be used as an effective additive in preventing marine fish blue scad surimi gel softening, which is quite possibly caused by myofibril-bound serine proteinase (MBSP).  相似文献   

Non-ideal Behavior During Complete Dissolution of Organic Immiscible Liquid: 2. Ideal Porous Media     

M. K. Mahal  Asami Murao  Gwynn R. Johnson  Ann E. Russo  Mark L. Brusseau 《Water, air, and soil pollution》2010,213(1-4):191-197

Column experiments were conducted using ideal natural sands and stainless-steel beads to examine the complete dissolution behavior of an organic immiscible liquid. Trichloroethene was used as the representative organic liquid. The elution curves exhibited multi-step behavior, with multiple extended periods of relatively constant contaminant flux. These secondary steady-state stages occurred at concentrations several orders-of-magnitude below aqueous solubility for the well-sorted sands. In contrast, the secondary steady-state stages occurred within 1 log of aqueous solubility for the poorly sorted sand. The non-ideal behavior is hypothesized to result from constraints to hydraulic accessibility of the organic liquid to flowing water, which may be expected to be mediated by the pore-scale configuration of the flow field and the fluid phases.  相似文献   

Influences of Forest Decline on Various Properties of Soils at Mt. Hirugatake, Tanzawa Mountains, Kanto District, Japan     

Kenji Ohse  Kenji Tamura  Haruo Tanaka  Teruo Higashi 《Soil Science and Plant Nutrition》2005,51(1):37-42

At Mt. Hirugatake in the Tanzawa Mountains, Kanto district, Japan, the deciduous broadleaved forests have rapidly declined. In our previous studies, we reported that the amount of soil organic matter had significantly decreased at the early and final stages of forest decline, and that the soil microbial biomass also showed a large decrease at these stages, suggesting that the composition of soil organic matter might have also changed with forest decline. To clarify the influences of forest decline on the composition of soil organic matter, the amount of humic substances, optical properties of humic acid, and the amount of soil carbohydrates in surface soils at different stages of forest decline were investigated. The amounts of humic acid and fulvic acid decreased to a lesser extent at the early and middle stages of forest decline, and showed a significant decrease at the final stage. As the amount of humin significantly decreased at the early stage, it was plausible that the distinct decrease in the total carbon content of the soil surface horizons at the early stage of forest decline was induced by the decrease in the amount of humin, and at the final stage, by the decrease in the amounts of humic acid and fulvic acid. The amount of soil carbohydrates did not change appreciably with forest decline although the soil organic matter content markedly decreased. It was suggested that most of the carbohydrates in the soil surface horizons were in a stabilized form consisting of complexes with humic substances, metals, and minerals, and would not be affected by the environmental changes associated with forest decline.  相似文献