Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758)          下载免费PDF全文

GL Lima  VB Luz  LF Lima  RMP Rocha  SV Castro  TS Castelo  APR Rodrigues  JR Figueiredo  AR Silva 《Reproduction in domestic animals》2018,53(4):880-888

The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions.  相似文献   

How does water and salt stress affect the germination and initial growth of Brazilian soya bean cultivars?     

Alan Mario Zuffo  Fábio Steiner  Tiago de Oliveira Sousa  Jorge González Aguilera  Paulo Eduardo Teodoro  Francisco de Alcântara Neto  Rafael Felippe Ratke 《Journal of Agronomy and Crop Science》2020,206(6):837-850

The effects of water and salt stress on rate of germination and seedling growth were investigated under laboratory conditions in 46 soya bean genotypes from Central-West region of Brazil to verify how these stresses may limit crop establishment during the initial growth stage and also to identify the most tolerant genotypes to drought and salinity. Mild water and salt stresses were imposed by seed exposure to –0.20 MPa iso-osmotic solutions with polyethylene glycol—PEG 6000 (119.57 g/L) or NaCl (2.357 g/L) for 12 days at 25°C. The germination percentage, seedling length and seedling dry matter were measured, and then, salt or drought tolerance indexes were calculated. The “NS 5909 RG,” “NS 7000 IPRO,” “NS 7338IPRO,” “FPS Solimões RR,” “NS 5151 IPRO,” “SYN 13610 IPRO,” “LG 60177 IPRO,” “NS 6909 IPRO” and “BMX Desafio RR” were identified as the most drought-tolerant genotypes, whereas under salinity conditions, the genotypes “5D 615 RR,” “BMX Desafio RR,” “5D 6215 IPRO” and “BMX Ponta IPRO” were identified as tolerant. The “BMX Desafio RR” is the genotype most adapted to both stress conditions and, therefore, should be used under conditions of water shortage and excess salt in the soil at sowing time.  相似文献   

Brewery residues as a source of organic carbon in Litopenaeus schmitti white shrimp farms with BFT systems     

Michelle Midori Sena Fugimura  Helaine dos Reis Flor  Emanuela Paula de Melo  Tiago Viana da Costa  Wilson Wasielesky Jr.  Lidia Miyako Yoshii Oshiro 《Aquaculture International》2015,23(2):509-522

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The effect of life history events on carcass merit and price of cull dairy cows     

Ligia C Moreira  Tiago L Passafaro  Daniel M Schaefer  Guilherme J M Rosa 《Journal of animal science》2021,99(1)

Cull dairy cows contribute almost 10% of national beef production in the United States. However, different factors throughout the life of dairy cows affect their weight and overall body condition as well as carcass traits, and consequently affect their market price. Therefore, the objectives of this study were: (1) to assess relationships between price ratio and carcass merit of cull dairy cows sold through several sites of an auction market and (2) to investigate the effect of animal life history events and live weight on sale barn price (BP) and price ratio (as a measure of relative price), as an indicator of carcass merit. Data from 4 dairy operations included 3,602 cull dairy cow records during the period of 2015 to 2019. Life history events data were collected from each dairy operation through Dairy Comp software; live weight and price were obtained periodically from the auction market, and the carcass data were provided by a local packing plant. Cow price in dollars per unit of live weight ($/cwt) and price ratio were the 2 outcome variables used in the analyses. Price ratio was created aiming to remove seasonality effects from BP (BP divided by the national average price for its respective month and year of sale). The association between price ratio and carcass merit traits was investigated using canonical correlation analysis, and the effect of life history events on both BP and price ratio was inferred using a multiple linear regression technique. More than 70% of the cows were culled in the first 3 lactations, with an average live weight of 701.5 kg, carcass weight of 325 kg, and dressing percentage of 46.3%. On average, cull cows were sold at $57.0/cwt during the period considered. The canonical correlation between price ratio and carcass merit traits was 0.76, indicating that price ratio reflected carcass merit of cull cows. Later lactations led to lower BP compared with cows culled during the first 2 lactations. Injury, and leg and feet problems negatively affected BP. Productive variables demonstrated that the greater milk production might lead to lower cow prices. A large variation between farms was also noted. In conclusion, price ratio was a good indicator of carcass merit of cull cows, and life history events significantly affected sale BP and carcass merit of cull cows sold through auction markets.  相似文献   

Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure     

Yoav Bashan  Alexander A. Kamnev  Luz E. de-Bashan 《Biology and Fertility of Soils》2013,49(4):465-479

Literature analysis and chemical considerations of biological phosphate solubilization have shown that the commonly used selection factor for this trait, tricalcium phosphate (TCP), is relatively weak and unreliable as a universal selection factor for isolating and testing phosphate-solubilizing bacteria (PSB) for enhancing plant growth. Most publications describing isolation of PSB employed TCP. The use of TCP usually yields many (up to several thousands per study) isolates “supposedly” PSB. When these isolates are further tested for direct contribution of phosphorus to the plants, only a very few are true PSB. Other compounds are also tested, but on a very small scale. These phosphates (P), mainly Fe-P, Al-P, and several Ca-P, are even less soluble than TCP in water. Because soils greatly vary by pH and several chemical considerations, it appears that there is no metal-P compound that can serve as the universal selection factor for PSB. A practical approach is to use a combination of two or three metal-P compounds together or in tandem, according to the end use of these bacteria—Ca-P compounds (including rock phosphates) for alkaline soils, Fe-P and Al-P compounds for acidic soils, and phytates for soils rich in organic P. Isolates with abundant production of acids will be isolated. This approach will reduce the number of potential PSB from numerous isolates to just a few. Once a potential isolate is identified, it must be further tested for direct contribution to P plant nutrition and not necessarily to general growth promotion, as commonly done because promotion of growth, even by PSB, can be the outcome of other mechanisms. Isolates that do not comply with this general sequence of testing should not be declared as PSB.  相似文献   

Outdoor mass microalgae production in Bahia Kino, Sonora, NW Mexico     

Domenico Voltolina  María del Pilar Snchez-Saavedra  Luz María Torres-Rodríguez 《Aquacultural Engineering》2008,38(2):93-96

The diatom Chaetoceros muelleri was grown in outdoor mass cultures under the winter, spring and summer conditions of Bahia Kino, Sonora, NW Mexico. The solar irradiance in winter was close to 60% of that available in spring and summer, but the cell concentrations and the harvestable biomass were one order of magnitude higher in spring and summer than in winter. There was no difference between the biomass harvested after 2 and 3 days in winter and summer, whereas in spring it was higher after 3 days. The protein content was significantly lower in winter, and carbohydrates and lipids were higher in winter and spring. The number of cells and the amount of harvestable biomass of outdoor cultures of C. muelleri depend on the temperature prevailing in each season, which causes significant differences in growth rates and in biochemical composition.  相似文献   

Molecular markers useful to discriminate <Emphasis Type="Italic">Coffea arabica</Emphasis> cultivars with high genetic similarity     

Tiago Vieira Sousa  Eveline Teixeira Caixeta  Emilly Ruas Alkimim  Antonio Carlos Baião de Oliveira  Antonio Alves Pereira  Laércio Zambolim  Ney Sussumu Sakiyama 《Euphytica》2017,213(3):75

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Potential Anti-Obesity,Anti-Steatosis,and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions     

Ana Regueiras  lvaro Huguet  Tiago Conde  Daniela Couto  Pedro Domingues  Maria Rosrio Domingues  Ana Margarida Costa  Joana Laranjeira da Silva  Vitor Vasconcelos  Ralph Urbatzka 《Marine drugs》2022,20(1)

Microalgae are known as a producer of proteins and lipids, but also of valuable compounds for human health benefits (e.g., polyunsaturated fatty acids (PUFAs); minerals, vitamins, or other compounds). The overall objective of this research was to prospect novel products, such as nutraceuticals from microalgae, for application in human health, particularly for metabolic diseases. Chlorella vulgaris and Chlorococcum amblystomatis were grown autotrophically, and C. vulgaris was additionally grown heterotrophically. Microalgae biomass was extracted using organic solvents (dichloromethane, ethanol, ethanol with ultrasound-assisted extraction). Those extracts were evaluated for their bioactivities, toxicity, and metabolite profile. Some of the extracts reduced the neutral lipid content using the zebrafish larvae fat metabolism assay, reduced lipid accumulation in fatty-acid-overloaded HepG2 liver cells, or decreased the LPS-induced inflammation reaction in RAW264.7 macrophages. Toxicity was not observed in the MTT assay in vitro or by the appearance of lethality or malformations in zebrafish larvae in vivo. Differences in metabolite profiles of microalgae extracts obtained by UPLC-LC-MS/MS and GNPS analyses revealed unique compounds in the active extracts, whose majority did not have a match in mass spectrometry databases and could be potentially novel compounds. In conclusion, microalgae extracts demonstrated anti-obesity, anti-steatosis, and anti-inflammatory activities and could be valuable resources for developing future nutraceuticals. In particular, the ultrasound-assisted ethanolic extract of the heterotrophic C. vulgaris significantly enhanced the anti-obesity activity and demonstrated that the alteration of culture conditions is a valuable approach to increase the production of high-value compounds.  相似文献   

Strategy to identify resistance to white mould associated with high yield for irrigated common bean in Brazil     

Renan C. Lima  Pablo H. Teixeira  Ari F. F. Souza  Trazilbo J. Paula Júnior  Hudson Teixeira  Miller S. Lehner  José E. S. Carneiro  Tiago S. Marçal  Rogerio F. Vieira 《Plant pathology》2020,69(6):1172-1184

Development of common bean cultivars with partial white mould resistance through breeding techniques has been a challenge in Brazil. As yet, lines/cultivars from breeding programmes have not been investigated for resistance; therefore, this study screened 107 lines/cultivars for their reactions to white mould in 14 preliminary trials conducted under irrigation. Thirteen resistant lines/cultivars (three of Andean origin) and six Mesoamerican cultivars (three intermediately resistant and three susceptible) were selected for further investigation. These lines/cultivars and the resistant control A195 were evaluated in six advanced trials and two straw tests to assess the effectiveness of the screening procedure. In 11 preliminary trials, screenings were performed under moderate/high or higher disease pressure. These pressures occurred in two advanced trials in which, when yields were averaged across moderate/high and high pressures, 10 Mesoamerican lines/cultivars selected for resistance yielded 14%, 23%, and 38% more than intermediately resistant cultivars, A195, and susceptible cultivars, with median disease ratings (1–9 scale) of 4.5, 5.7, 5.7, and 6.7, respectively. In the straw test, three Andean lines/cultivars (A195 included) and two susceptible cultivars in the field were among those with the highest levels of physiological resistance. Thus, field rating under high disease pressure and greenhouse rating did not correlate significantly, suggesting that field trials are critical to evaluating resistance and to identifying high-yielding beans. Therefore, lines/cultivars from breeding programmes assessed in field trials may provide a low cost and fast way to identify high-yielding bean cultivars with partial resistance to white mould in the subtropical southern hemisphere.  相似文献   

Genes related to flooding tolerance during germination and early growth of weedy rice     

Tiago Edu Kaspary  Luan Cutti  Rafael Schwalm Rafaeli  Carla Andrea Delatorre  Aldo Merotto Jr 《Weed Research》2020,60(6):435-449

Evolution of flooding tolerance in weedy rice has occurred in several rice-growing regions, but the genes related to this process and the environmental effects are unknown. The objective of this study was to analyse the expression of genes related to flooding tolerance in response to temperature and flooding during the initial establishment of weedy rice. The experiments were carried out with rice cultivars IRGA 417 and Nipponbare, which are sensitive to flooding, and weedy rice ITJ03 and AV04 genotypes that have high and intermediate tolerance to flooding, respectively. The expression of genes related to reserve mobilisation, anaerobic respiration, escape and quiescence strategies was analysed at periods up to 24 days after sowing. The flooding tolerance of weedy rice genotype ITJ03 was associated with the expression of RAmy3D and OsTPP7 , which are involved in the mobilisation of carbohydrate reserves, ADH1 and ADH2, which participate in anaerobic respiration, and SNRKL1 that triggers rapid elongation of the coleoptile and emergence. Although the genes PDC1, SUS3 and SUB1 are important for flooding tolerance in cultivated rice, their expression was not directly related to flooding tolerance in weedy rice. A temperature of 20°C reduced levels of expression of the RAmy3D, ADH2 and SNRKL1 genes and low temperature had a negative effect on the establishment of weedy rice. Breeding of rice genotypes with tolerance of low temperatures and anaerobic conditions may be a viable strategy to improve the control of weedy rice in paddy fields.  相似文献