Identification and distribution of a novel Malassezia species yeast on normal equine skin     

Nell A  James SA  Bond CJ  Hunt B  Herrtage ME 《The Veterinary record》2002,150(13):395-398

This study aimed to investigate the distribution of Malassezia species yeasts on the skin of healthy horses. Acetate tape samples were obtained from the lip, axilla, interbulbar region, groin and anus of 12 healthy horses. The samples were stained and examined microscopically and sites harbouring yeast-like organisms were identified. Contact plates were applied to the skin at these sites and cultured at 26 degrees C and 32 degrees C. No growth was obtained on horse blood, Sabouraud's dextrose or modified Dixon's agar. A pure growth of a Malassezia-type organism was obtained on Sabouraud's dextrose agar enriched with oleic acid when it was incubated at 30 degrees C. It was identified by 26S ribosomal DNA D1/D2 sequence analysis as a member of the genus Malassezia, and most closely related to Malassezia sympodialis. However, the level of sequence divergence indicated that it was a novel species.  相似文献   

Goniodysgenesis associated with primary glaucoma in an adult European Short-haired cat     

Katrin Trost  Robert L. Peiffer Jr  †  Barbara Nell 《Veterinary ophthalmology》2007,10(S1):3-7

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Comparison of pulmonary artery and transpulmonary thermodilution cardiac output measurements in unsedated newborn calves          下载免费PDF全文

Annette PN Kutter  Rahel S Jud Schefer  Barbara Bircher  Uli Bleul  Regula Bettschart‐Wolfensberger 《Veterinary anaesthesia and analgesia》2015,42(6):614-622

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Salinity studies on the clams Katelysia rhytiphora (Lamy) and Tapes dorsatus (Lamarck)     

J A Nell  K J Paterson 《Aquaculture Research》1997,28(2):115-119

The estuarine clams Katelysia rhytiphora (Lamy) and Tapes dorsatus (Lamarck) require salinities of 20–45%₀ for survival and of 25–35%₀ and 30–40%₀ respectively for best growth. The highest 24-h algae clearance rates for K. rhytiphora and T. dorsatus were observed at salinities of 25–35%₀ and 30–35%₀ respectively. Neither species survived longer than 7 days at salinities <20%₀ and they can. therefore, only be grown in the high-salinity parts of estuaries. The salinity requirements of these clams should be a primary consideration when selecting farming sites. Other species of bivalves that tolerate lower salinities should also be considered for farming in brackish water areas of New South Wales, Australia.  相似文献   

Hatchery production of diploid and triploid clams, Tapes dorsatus (Lamarck 1818): a potential new species for aquaculture     

John A. Nell  Wayne A. O'Connor  Rosalind E. Hand  Stuart P. McAdam 《Aquaculture (Amsterdam, Netherlands)》1995,130(4):389-394

To assess the potential for hatchery production of the venerid clam, Tapes dorsatus, diploid and triploid clams were produced and ongrown until the spat reached 5–8 mm in size. Triploidy percentages at metamorphosis that ranged from 56 to 85% were induced using a 15-min exposure to 1 mg/l cytochalasin B. No differences were observed in the growth rate of sibling diploid and triploid T. dorsatus larvae. Survival of diploid clams to pediveliger stage was however higher. Post-metamorphic growth and survival of diploid and triploid clams remained the same until the clams were removed from the hatchery to outdoor nurseries. Throughout the larval and early spat phases of production, T. dorsatus growth was similar to that reported for the Manila clam, Tapes philippinarum. Based upon its ease of culture, rapid growth and marketability, T. dorsatus are thought to have considerable aquaculture potential.  相似文献   

Plasma volume and electrolyte changes following intravenous infusion of hypertonic hydroxyethyl starch versus mannitol in healthy dogs     

Robinson R  Schwendenwein I  Wacek S  Nell B  Mosing M 《Veterinary journal (London, England : 1997)》2011,190(2):268-272

In a prospective cross-over study, the duration and magnitude of effect on the electrolyte and plasma volume changes of intravenous (IV) hypertonic hydroxyethyl starch (hyperHES) (7.5%/6%) and mannitol (20%) were compared. Eight Beagle dogs received an IV infusion of 4mL/kg hyperHES (group H) and 4mL/kg mannitol 20% (group M) on separate occasions. Urine and blood samples were taken in the first (T(60)) and second (T(120)) hour after infusion. Significant increases in plasma volume at each time point in group H and M were noted when compared to baseline (start of infusion=T(0)) level. There was no significant difference between groups. Both fluids resulted in diuresis, although no significant difference between groups was noted. A significant increase in plasma sodium (Na) was demonstrated in group H between T(0) and T(60) with a significant increase in the Na and chloride (Cl) fractional excretion (FE) between T(0), T(60) and T(120). In group M no changes in plasma electrolyte concentrations were detected, although FE of Na, Cl and K was increased significantly between T(0) and T(60). In conclusion, hyperHES and mannitol appear to have a volume expanding effect lasting at least 120 min. The hypernatraemia induced by hyperHES was minimal compared to previous reports of hypertonic saline use, and no clinical side effects were noted. HyperHES showed comparable effects to mannitol in increasing plasma volume and diuresis and could be considered for these applications.  相似文献   

Macrophakia in three cats     

Benz P  Walde I  Gumpenberger M  Nell B 《Veterinary ophthalmology》2011,14(Z1):99-104

This report describes three cases of bilateral macrophakia in 3-, 5-, and 9-year-old cats, respectively. All cats were presented because of visual deficits. Ophthalmic examination revealed macrophakia (the vitreous was replaced by the lens) and retinal changes (tapetal hyper-reflectivity, attenuation of retinal vessels, and retinal folds) in all cats. In addition, bilateral subconjunctival orbital fat prolapse in one cat and microphthalmos in another cat were present. To confirm the ophthalmologic diagnosis of macrophakia, gross pathology examination in one cat and ultrasound examination in another cat were performed.  相似文献   

Correlation between corneal sensitivity and quantity of reflex tearing in cows,horses, goats,sheep, dogs,cats, rabbits,and guinea pigs     

Barbara Wieser  Alexander Tichy  Barbara Nell 《Veterinary ophthalmology》2013,16(4):251-262

Objective Guinea pigs have a very low threshold of corneal sensitivity and at the same time nearly no reflex tearing compared to dogs, cats, and horses. The question arose whether there is a general correlation between corneal sensitivity and the quantity of reflex tearing. Animals studied Totally 160 animals of 8 different species (20 animals per species) were investigated. Procedures The corneal touch threshold (CTT) was measured with a Cochet–Bonnet esthesiometer. The palpebral fissure length (PFL) was measured with a calliper ruler. The Schirmer tear test (STT) was modified by adapting the width of the STT strip to the PFL of every species. For the STT II, 0.4% oxybuprocaine was applied. Results Corneal touch threshold: Cows (1.67 g/mm²), horses (1.23 g/mm²), sheep (1.13 g/mm²), goats (1.44 g/mm²), dogs (2.16 g/mm²), and cats (1.33 g/mm²) show similar CTT values. In contrast, rabbits (6.21 g/mm²) and guinea pigs (7.75 g/mm²) show a significantly lower CTT. Tear Production Difference STT I ? STT II: Rabbits have the greatest decline in tear production with 38.4%, followed by sheep (33.3%), dogs (31.1%), cats (24.7%), cows (23.7%), horses (18.0%), and goats (14.0%). Guinea pigs have no decline, but a slight increase of ?16.0%. Correlation CTT and STT II ? STT I Difference: Pearson’s correlation coefficient shows a small, but significant correlation. The coefficient of determination can only forecast a value with 7.1% certainty. Conclusions The high variance and low reproducibility of results suggest that the measuring devices are inappropriate to assess the evaluated parameters. Therefore, no assured correlation between the corneal sensitivity and the quantity of reflex tearing could be found.  相似文献   

Quantitative,noninvasive assessment of intra‐ and extraocular perfusion by contrast‐enhanced ultrasonography and its clinical applicability in healthy dogs     

Klaas‐Ole Blohm  Katharina M. Hittmair  Alexander Tichy  Barbara Nell 《Veterinary ophthalmology》2019,22(6):767-777

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Review of the measuring precision of the new Meibometer® MB550 through repeated measurements in dogs     

Petra Benz  Alexander Tichy  Barbara Nell 《Veterinary ophthalmology》2008,11(6):368-374

Objective A meibometer is a device to measure the delivery rate of lipids on the eyelid margin. The aim of this study is to determine the measuring precision of the new Meibometer^® MB550 (Courage-Khazaka electronic GmbH, 50829, Cologne, Germany), linked to a computer, by means of repeated measurements in dogs by different examiners. Procedure Two investigators measured the lipid rate on the eyelid margin in 10 healthy dogs for 10 days. One examiner measured the right eye (OD) and the other measured the left eye (OS) for 5 days. After 5 days, the eyes to be measured were switched between the examiners. The new device was able to record all measurement values as charts and curves in comparison to the previous Meibometer, which displayed only one value. Results Mean meibomium lipid level ± SD in the OD and OS of 10 dogs in 10 days was 211 ± 48 MU and 205 ± 41 MU (meibometer units), respectively. There was no significant difference between OD and OS, but a broad distribution of the values could be found. Gender did not influence the measurements. A significant difference was determined between the measurements in the first 5 days to those in the following 5 days. There was, therefore, also a significant difference in the measurements of the 2 examiners. Conclusion Meibometry is a minimally invasive lipid measuring method on the eyelid margin. Repeated measurement results obtained by two examiners, with the new device Meibometer MB550 linked to a computer, showed a wide range of values. The measuring precision of the new Meibometer MB550 is therefore questionable.  相似文献