Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.          下载免费PDF全文

K F Lawson  H Chiu  M Matson  P Bachmann    J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.  相似文献   

Epidemiology of contagious pleuropneumonia in hogs     

P Bachmann 《Schweizer Archiv für Tierheilkunde》1972,114(7):362-382

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Risk factors associated with behavioural disorders of crib-biting,weaving and box-walking in Swiss horses     

Bachmann I  Audigé L  Stauffacher M 《Equine veterinary journal》2003,35(2):158-163

REASONS FOR PERFORMING STUDY: Studies on the prevalence of behavioural disorders in horses and on associated risk factors have revealed inconsistent results. There are many studies on the neuropharmacological, surgical or mechanical therapy of stereotypies, but little is known about their causation. OBJECTIVES: To explore risk factors associated with the occurrence of behavioural disorders in horses. METHODS: A sample of horse owners, selected randomly and representative for Switzerland, was contacted in a postal survey. Answers were provided for 622 stables (response rate 35.2%). Individual data of 2,341 horses were examined with path analysis (multivariable linear and logistic regression), and adjustment made for possible confounding effects due to age and breed. RESULTS: Out of 60 possible risk factors, 11 were associated with the outcome at the univariable level (null-hypothesis path model) and 3 factors remained after the backward logistic regression procedure. Mature Warmbloods and Thoroughbreds, assessed by the owners to be reactive, fed 4 times a day and without daily pasture, had increased odds of displaying crib-biting, weaving and box-walking. Furthermore, indirect associations of 5 factors with the outcome were identified. CONCLUSIONS: The final logistic regression model of risk factors leads to the hypotheses that causal prevention of stereotypic behaviours should be based upon housing and management conditions which allow tactile contact with other horses (e.g. mutual grooming), daily free movement (paddock or pasture), as well as the provision of high amounts of roughage but of little or no concentrates. POTENTIAL CLINICAL RELEVANCE: It is one of the aims of population medicine to prevent the development of behavioural disorders. Further research is needed to test the concluding hypotheses in experimental studies or to verify them in the context of similar observational studies.  相似文献   

Vaccination of weaner pigs against classical swine fever with the subunit vaccine "Porcilis Pesti": influence of different immunization plans on excretion and transmission of challenge virus     

Ziegler U  Kaden V 《Berliner und Münchener tier?rztliche Wochenschrift》2002,115(7-8):267-273

Excretion and transmission of CSFV after vaccination with the CSF subunit marker vaccine "Porcilis Pesti" have been studied using the following different vaccination schedules: Group A--two vaccinations with an interval of 28 d, challenge 14 d after second vaccination (p.v2.); group B--two vaccinations with an interval of 14 d, challenge 14 d later; group C--two vaccinations with an interval of 28 d, challenge at time of booster vaccination; group D--two vaccinations with an interval of 14 d, challenge 7 d p.v2.; group E--single vaccination and infection 14 d later. After infection one sentinel pig was added to the vaccinated and infected pigs of each group. A single vaccination did not induce protective immunity against a CSFV challenge. Double vaccination at a four-week interval protected piglets from clinical infection, and neither viraemia and leukopenia nor virus excretion were detected if infected 14 d p.v2. Two vaccinations at a two-week interval followed by a challenge 7 d p.v2. led to a short viraemia on day 5 p.i. but without excretion of CSFV. Though all other vaccination schedules induced a reduction in virus shedding and a decrease in CSFV replication, in all these cases in-contact controls became infected. The results of transmission of CSFV are discussed in relation to a potential use of subunit marker vaccines in CSF control.  相似文献   

Blume,H.-P. und U. Schleuß (Hrsg.): Bewertung anthropogener Stadtböden, 1997, Band 38, Schriftenreihe Institut für Pflanzenernährung und Bodenkunde der Universität Kiel (Hrsg.: Blume,H.-P./Horn,R./Sattelmacher,B.) ISSN 0933–680 x. Paperback, 346 Seiten,DM 30,-     

Jrg Bachmann 《植物养料与土壤学杂志》1998,161(6):699-699

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Bedeutung eines jahreszeitlich unterschiedlichen Einschlagszeitpunktes für das Trocknungsverhalten des Schnittholzes von Fichten (Picea abies (L.) Karst.)     

Volker Klaiber und Ute Seeling 《Forstwissenschaftliches Centralblatt》2002,121(6):267-282

Zusammenfassung:   Die vorliegende Untersuchung hat zum Ziel, die Auswirkungen eines jahreszeitlich unterschiedlichen Einschlagszeitpunktes von Fichten (Winter- und Sommereinschlag) auf die Dimensionsstabilität des daraus erzeugten Schnittholzes zu erfassen und zu quantifizieren. Hierzu wurden jeweils 12 Fichten aus Winter- und Sommereinschlag unmittelbar nach der Aufarbeitung im Bestand zu Bauschnittholz in praxisüblichen Dimensionen verarbeitet. Die Erfassung der trocknungsbedingten Verwerfungen des Schnittholzes erfolgte mittels Vermessung der Kantholzform vor und nach einer technischen Kammertrocknung (Zielfeuchte u = 15 ± 3%). Dabei zeigte sich, dass der saisonal unterschiedliche Zeitpunkt der Fällung der Bäume keinen statistisch abgesicherten Einfluss auf die durchschnittlichen Verwerfungen bzw. die Dimensionsstabilität der daraus erzeugten Kanthölzer hatte.  相似文献   

PROGRESS ON IMPROVING AGRICULTURAL NITROGEN USE EFFICIENCY: UK-CHINA VIRTUAL JOINT CENTERS ON NITROGEN AGRONOM     

Tom MISSELBROOK  Zhaohai BAI  Zejiang CAI  Weidong CAO  Alison CARSWELL  Nicholas COWAN  Zhenling CUI  David CHADWICK  Bridget EMMETT  Keith GOULDING  Rui JIANG  Davey JONES  Xiaotang JU  Hongbin LIU  Yuelai LU  Lin MA  David POWLSON  Robert M. REES  Ute SKIBA  Pete SMITH  Roger SYLVESTER-BRADLEY  John WILLIAMS  Lianhai WU  Minggang XU  Wen XU  Fusuo ZHANG  Junling ZHANG  Jianbin ZHOU  Xuejun LIU 《农业科学与工程前沿(英文版)》2022,9(3):475

● Virtual joint centers on N agronomy were established between UK and China. ● Key themes were improving NUE for fertilizers, utilizing livestock manures, and soil health. ● Improved management practices and technologies were identified and assessed. ● Fertilizer emissions and improved manure management are key targets for mitigation. Two virtual joint centers for nitrogen agronomy were established between the UK and China to facilitate collaborative research aimed at improving nitrogen use efficiency (NUE) in agricultural production systems and reducing losses of reactive N to the environment. Major focus areas were improving fertilizer NUE, use of livestock manures, soil health, and policy development and knowledge exchange. Improvements to fertilizer NUE included attention to application rate in the context of yield potential and economic considerations and the potential of improved practices including enhanced efficiency fertilizers, plastic film mulching and cropping design. Improved utilization of livestock manures requires knowledge of the available nutrient content, appropriate manure processing technologies and integrated nutrient management practices. Soil carbon, acidification and biodiversity were considered as important aspects of soil health. Both centers identified a range of potential actions that could be taken to improve N management, and the research conducted has highlighted the importance of developing a systems-level approach to assessing improvement in the overall efficiency of N management and avoiding unintended secondary effects from individual interventions. Within this context, the management of fertilizer emissions and livestock manure at the farm and regional scales appear to be particularly important targets for mitigation.  相似文献   

Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes,Actinokineospora sp. EG49 and Nocardiopsis sp. RV163     

Yousef Dashti  Tanja Grkovic  Usama Ramadan Abdelmohsen  Ute Hentschel  Ronald J. Quinn 《Marine drugs》2014,12(5):3046-3059

Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by ¹H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 1–10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.  相似文献   

Metabolomic Tools for Secondary Metabolite Discovery from Marine Microbial Symbionts     

Lynsey Macintyre  Tong Zhang  Christina Viegelmann  Ignacio Juarez Martinez  Cheng Cheng  Catherine Dowdells  Usama Ramadan Abdelmohsen  Christine Gernert  Ute Hentschel  RuAngelie Edrada-Ebel 《Marine drugs》2014,12(6):3416-3448

Marine invertebrate-associated symbiotic bacteria produce a plethora of novel secondary metabolites which may be structurally unique with interesting pharmacological properties. Selection of strains usually relies on literature searching, genetic screening and bioactivity results, often without considering the chemical novelty and abundance of secondary metabolites being produced by the microorganism until the time-consuming bioassay-guided isolation stages. To fast track the selection process, metabolomic tools were used to aid strain selection by investigating differences in the chemical profiles of 77 bacterial extracts isolated from cold water marine invertebrates from Orkney, Scotland using liquid chromatography-high resolution mass spectrometry (LC-HRMS) and nuclear magnetic resonance (NMR) spectroscopy. Following mass spectrometric analysis and dereplication using an Excel macro developed in-house, principal component analysis (PCA) was employed to differentiate the bacterial strains based on their chemical profiles. NMR ¹H and correlation spectroscopy (COSY) were also employed to obtain a chemical fingerprint of each bacterial strain and to confirm the presence of functional groups and spin systems. These results were then combined with taxonomic identification and bioassay screening data to identify three bacterial strains, namely Bacillus sp. 4117, Rhodococcus sp. ZS402 and Vibrio splendidus strain LGP32, to prioritize for scale-up based on their chemically interesting secondary metabolomes, established through dereplication and interesting bioactivities, determined from bioassay screening.  相似文献   

Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs   总被引：8，自引：0，他引：8  

H U Bertschinger  M Bachmann  C Mettler  A Pospischil  E M Schraner  M Stamm  T Sydler  P Wild 《Veterinary microbiology》1990,25(2-3):267-281

Two strains of E. coli O139:K12 (B):H1 were compared in vitro and in the intestinal environment. Both strains colonized the small intestines of experimentally inoculated pigs and exhibited in vivo a similar relationship to the microvillus border as enterotoxigenic E. coli (ETEC). Strain 107/86 grown on blood agar expressed numerous long flexible non-haemagglutinating fimbriae which were antigenically distinct from the known fimbriae of porcine ETEC. It adhered in vitro to porcine enterocyte brush border fragments. Strain 124/76 grown on blood agar was devoid of fimbriae and did not adhere to brush border fragments. However, fimbriae morphologically and antigenically indistinguishable from those of strain 107/86 were detected in the intestinal environment by direct immunofluorescence and by immuno electron microscopy.  相似文献