Remediation of metal-contaminated mine tailings by the application of organic and mineral amendments     

Benidire  Leila  Pereira  Sofia  Aboudrar  Wafae  Hafidi  Mohamed  Castro  Paula  Boularbah  Ali 《Journal of Soils and Sediments》2022,22(2):482-495

Journal of Soils and Sediments - Tailings are generally characterized by severe physicochemical conditions that limit the establishment of vegetation. The present study aimed to select suitable...  相似文献   

Effect of nickel-resistant rhizosphere bacteria on the uptake of nickel by the hyperaccumulator Noccaea caerulescens under controlled conditions     

Wafae Aboudrar  Christophe Schwartz  Jean Louis Morel  Ali Boularbah 《Journal of Soils and Sediments》2013,13(3):501-507

Purpose

This work was conducted to determine whether Nickel-resistant rhizosphere bacteria (NiRRB) affected the Ni uptake and hyperaccumulation by Noccaea caerulescens.

Material and methods

Ni-resistant bacterial strains (10 mM Ni), Bacillus pumilus and Micrococcus spp., were originally isolated from the rhizosphere of N. caerulescens (Brassicaceae) collected from serpentine Ni-rich soil in the Vosges mountains (France). They served to inoculate axenic seeds of N. caerulescens, which were then sown aseptically into sterile soil Magenta boxes and cultured under controlled conditions.

Results and discussion

Cultures inoculated with NiRRB showed a disappearance of chlorosis signs and a significant increase of the aerial biomass compared to the axenic control (1.5-fold higher). Despite the drastic decrease of the water-extractable Ni in the soil (80 %) at the end of the growth, there was no significant effect on Ni accumulation by N. caerulescens compared to the axenic control. A highly significant promoting effect on plant growth (fourfold higher) and an increase of Ni accumulation (65-fold higher) by N. caerulescens were shown in cultures with native microorganisms (nonsterile control) compared to the axenic control.

Conclusions

The NiRRB strains used in this study reduced Ni bioavailability in the soil and did not improve the Ni uptake by N. caerulescens. Nevertheless, they induced promoting effects on the growth of this plant. They could serve as an effective metal-immobilizing and growth-promoting bioinoculant for plants in Ni-stressed soils. Optimal growth and Ni uptake by N. caerulescens requires the presence of native microbial community.  相似文献   

Morphometric study of the tricuspid valve in dogs     

Alves JR  Wafae N  Beu CC  Tuzuki LC  Ruiz CR  Wafae GC 《Anatomia, histologia, embryologia》2008,37(6):427-429

An anatomical study of the tricuspid valve was performed in the hearts of 45 dogs using a method of dissection which preserves the integrity of the valve. Morphometrical criteria such as the width of the cusp base and larger depth of cusp and morphological criteria such as the number of tendinous cords, the perimeter of the fibrous ring and the properties of the papillary muscle were adopted for the characterization of a cusp. The tricuspid valve was not consistent with three cusps, but it was observed to present 2, 4 or 5 cusps in 68% of the cases.  相似文献