全文获取类型
收费全文 | 359篇 |
免费 | 3篇 |
国内免费 | 2篇 |
专业分类
林业 | 30篇 |
农学 | 5篇 |
53篇 | |
综合类 | 9篇 |
农作物 | 9篇 |
水产渔业 | 38篇 |
畜牧兽医 | 200篇 |
园艺 | 3篇 |
植物保护 | 17篇 |
出版年
2022年 | 4篇 |
2021年 | 25篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 8篇 |
2015年 | 27篇 |
2014年 | 19篇 |
2013年 | 23篇 |
2012年 | 17篇 |
2011年 | 23篇 |
2010年 | 14篇 |
2009年 | 18篇 |
2008年 | 21篇 |
2007年 | 28篇 |
2006年 | 23篇 |
2005年 | 16篇 |
2004年 | 28篇 |
2003年 | 19篇 |
2002年 | 15篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
排序方式: 共有364条查询结果,搜索用时 31 毫秒
1.
Takaki Waritani Yoko Okuno Yoshinori Ashida Ryo Tsuchiya Kosaku Kobayashi Takatsugu Yamada 《Veterinary immunology and immunopathology》2001,80(3-4):333-338
Canine cationic trypsin was purified by salting-out, gel filtration and affinity chromatography. Purity was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight was ca. 28 kDa by SDS-PAGE.
Thirty hybridomas were obtained which produced mAb to canine cationic trypsin by the cell fusion technique. Twenty-two of these recognized cationic trypsin only, while eight hybridomas recognized both cationic and anionic trypsin. Several of the anti-canine cationic trypsin mAb were purified by salting-out and DEAE ion-change chromatography using ascites fluid of immunized BALB/c mice. The mAb proved to have very high specificity to canine cationic trypsin as shown by immunoblotting and it may be possible to use them to develop clinical assays. 相似文献
2.
Kyutaro Kishimoto Yoko Nishizawa Yutaka Tabei Masami Nakajima Tadaaki Hibi Katsumi Akutsu 《Journal of General Plant Pathology》2004,70(6):314-320
A class III chitinase gene (CHI2) is induced in cucumber plants (Cucumis sativa L.) in response to infection by pathogenic microorganisms. Infection of Botrytis cinerea, causal agent of gray mold disease on cucumber, also induces CHI2 expression. To investigate whether CHI2 is involved in resistance to gray mold disease, transgenic cucumber plants were produced to overexpress the CHI2 gene. One line was analyzed in detail in terms of disease resistance. The transgenic cucumber plant (CC2) constitutively expressed CHI2 and reduced the symptoms of B. cinerea for 4 days after inoculation compared with nontransgenic plants. However, this inhibitory effect was not absolute, and CC2 eventually developed serious disease symptoms. Chitinase activity of the crude extract from CC2 leaves was higher than that from nontransgenic plants. A high-molecular-weight fraction containing CHI2 from CC2 leaves had fungistatic activity against B. cinerea. Interestingly, the low-molecular-weight fraction from CC2 leaves with CHI2 removed also had fungistatic activity against B. cinerea. Not only the introduced chitinase activity but also the endogenous defense reactions activated by overexpression of CHI2 may be involved in the enhanced gray mold disease resistance in CC2. 相似文献
3.
A questionnaire study examining Japanese consumers’ requirements for beef quality was carried out in April 2002 in Tsukuba, Ibaraki, Japan. Answers from 532 participants were used for the statistical analysis. Twenty questions about sensory properties, appearance, safety and freshness, production area, price, and other properties were prepared. The answers were applied to principal component analysis. Five principal components were obtained and defined, and the principle component scores were used to classify participants. The panels were classified into four groups profiled as ‘specific quality orientated’, ‘Wagyu (Japanese beef) orientated’, ‘safety and freshness orientated’ and ‘overall high quality orientated’. In addition, gender and age characteristics were different among the groups. Sensory properties did not affect the classification of respondents. 相似文献
4.
Keisuke SASAKI Tomotaro YOSHIDA Kengo KOGA Takashi HARAGUCHI Kazunori OHASHI Yosuke AOYAGI 《Animal Science Journal》2004,75(6):557-562
The effect of insulin on the ascorbate recycling system in the chicken liver was examined. First, insulin was injected subcutaneously into the chicken, and liver glutathione‐dependent dehydroascorbate reductase (GSH‐DHAR) activity was determined. Insulin increased liver GSH‐DHAR activity, but did not affect plasma and liver ascorbate concentration. Dehydroascorbate increased plasma and liver ascorbate levels, and liver GSH‐DHAR activity. However, distinct changes in plasma insulin level were not observed by dehydroascorbate injection. In addition, reduction of external dehydroascorbate in cultured chicken hepatocytes could not be observed in an insulin‐deprived culture, although the cells reduced external dehydroascorbate in a serum‐free culture with insulin. We concluded that insulin affects the ascorbate recycling system as an essential factor in the chicken liver. 相似文献
5.
Yosuke SASAKI Satoshi SEKIGUCHI Ryoko UEMURA Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):333-335
This study compared the reproductive and growth performances of pigs before and after depopulation and
restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after
depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and
restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and
respiratory syndrome and Aujeszky’s disease, remained undetected on the farms. Compared with before
depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born
was higher, the postweaning mortality rate was lower, and the age at slaughter was lower
(P<0.05). In summary, depopulation and restocking improved the reproductive and growth
performances of pigs. 相似文献
6.
Kazuki HARADA Atsushi SASAKI Takae SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):83-89
The study objective was to determine the effect of oral orbifloxacin (ORB) on
antimicrobial susceptibility and composition of fecal coliforms in cats. Nine cats were
randomized to two groups administered a daily oral dose of 2.5 and 5.0 mg ORB/kg for 7
days and a control group (three cats per group). Coliforms were isolated from stool
samples and were tested for susceptibilities to ORB and 5 other drugs. ORB concentration
in feces was measured using high-performance liquid chromatography (HPLC). The coliforms
were undetectable after 2 days of ORB administration, and their number increased in most
cats after termination of the administration. Furthermore, only isolates of
Escherichia coli were detected in all cats before administration, and
those of Citrobacter freundii were detected after termination of the
administration. E. coli isolates exhibited high ORB susceptibility
[Minimum inhibitory concentration (MIC), ≤0.125 µg/ml]
or relatively low susceptibility (MIC, 1−2 µg/ml) with a
single gyrA mutation. C. freundii isolates largely
exhibited intermediate ORB susceptibility (MIC, 4
µg/ml), in addition to resistance to ampicillin and
cefazolin, and harbored qnrB, but not a gyrA mutation.
HPLC revealed that the peaks of mean concentration were 61.3 and 141.0
µg/g in groups receiving 2.5 and 5.0 mg/kg, respectively. Our findings
suggest that oral ORB may alter the total counts and composition of fecal coliform, but is
unlikely to yield highly fluoroquinolone-resistant mutants of E. coli and
C. freundii in cats, possibly because of the high drug concentration in
feces. 相似文献
7.
Paddy and Water Environment - In Japan, since ancient times, numerous small- to medium-size agricultural ponds have been constructed to irrigate paddy rice fields. Economic growth and urbanization... 相似文献
8.
Ahram KIM Naoki SASAKI Inhyung LEE Jong-pil SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):643
The purpose of this study was to assess the cardiorespiratory and behavioral responses to the combination of medetomidine and tramadol (M-T) or butorphanol (M-B) in standing laparoscopic ovariectomy in horses. One ovary was removed under M-T and the contralateral ovary was removed under M-B with at least 4 weeks between operations at random. Horses were sedated using intravenous medetomidine (5 µg/kg) followed by tramadol (1 mg/kg) or butorphanol (10 µg/kg) after 5 min. Sedation was maintained through the repeated injection of medetomidine (1 µg/kg) and tramadol (0.4 mg/kg) or medetomidine (1 µg/kg) and butorphanol (4 µg/kg) every 15 min. Cardiorespiratory function and behavioral responses, including, sedation, ataxia, and analgesia, were assessed during the surgery. There were no significant differences in cardiorespiratory values and sedation and analgesia scores between M-T and M-B. Ataxia scores were significantly lower in M-T than in M-B. This result suggests that M-T could maintain smooth and stable standing surgery with minimal cardiorespiratory changes in horses. 相似文献
9.
Expression dynamics of bovine MX genes in the endometrium and placenta during
early to mid pregnancy
Takahiro SHIROZU Keisuke SASAKI Manabu KAWAHARA Yojiro YANAGAWA Masashi NAGANO Nobuhiko YAMAUCHI Masashi TAKAHASHI 《The Journal of reproduction and development》2016,62(1):29-35
MX belongs to a family of type I interferon (IFN)-stimulated genes, and the MX protein has
antiviral activity. MX has at least two isoforms, known as MX1 and
MX2, in mammals. Moreover, bovine MX1 has been found to have alternative
splice variants—namely, MX1-a and MX1B. In ruminants, IFN-τ—a type I IFN—is
temporarily produced from the conceptus before implantation and induces MX expression in the
endometrium. However, the expression dynamics of MX after implantation are not clear. In the
present study, we investigated the expression of MX1-a, MX1B and
MX2 in the endometrium and placenta before and after implantation along with the expression
of IFN-α, type I receptors (IFNAR1 and IFNAR2) and
interferon regulatory factors (IRF3 and IRF9). Pregnant uterine samples were
divided into five groups according to pregnancy days 14–18, 25–40, 50–70, 80–100, and 130–150. Tissue samples
were collected from the intercaruncular endometrium (IC), caruncular endometrium (C) and fetal placenta (P).
Although all the MX expressions were significantly higher in the IC and C at days 14–18,
presumably caused by embryo-secreted IFN-τ stimulation, their expressions were also detectable in the IC, C
and P after implantation. Furthermore, IFN-α expression was significantly higher in the IC.
RT-PCR indicated IFNAR1, IFNAR2, IRF3 and
IRF9 mRNA in all the tissues during pregnancy. These results suggest that all the
MX genes are affected by the type I IFN pathway during pregnancy and are involved in an
immune response to protect the mother and fetus. 相似文献
10.
The aim of the present study was to examine whether cumulus and fibroblast cell nuclear-transferred oocytes, which have high and low potential to develop into normal calves, respectively, are different in terms of in their patterns of timing of first cleavage and in their relationships between timing of first cleavage and in vitro developmental potential. The timing of first cleavage was similar in both types of nuclear-transferred and in vitro fertilized oocytes. More than 86% of the oocytes cleaved within 24 h after activation or in vitro fertilization; these oocytes contributed to more than 98% of the total number of blastocysts in all three groups. The potential of oocytes that cleaved at different intervals to develop into blastocysts differed among the groups. The developmental potential of the cumulus cell nuclear-transferred oocytes and in vitro fertilized oocytes decreased with the increase in time required for cleavage. Fibroblast cell nuclear-transferred oocytes that cleaved at 20 h, an intermediate cleaving time, had higher potential to develop into blastocysts. The results of the present study suggest that the type of donor nucleus used for nuclear transfer affects the timing of first cleavage. 相似文献