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1.
Ookawa K Mochizuki K Sugimoto Y Suzuki T Hashizume M Kitagawa T Matumoto T Ito C Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):49-54
Lipid extracted from the ovary of skipjack tuna by the method that we developed is rich in phospholipid-type docosahexaenoic acid. The ovary lipid of skipjack tuna (OLS) was studied for its anti-stress activity in male Wistar rats, focusing on stress-related blood components: recovery from stress was examined after application of water immersion restraint stress. As a result, serum corticosterone (CORT) secretion was inhibited and decreased rapidly after stress application in rats given OLS compared with control rats. As CORT acts as a glucocorticoid, non-esterified fatty acid (NEFA) is expected to increase by stress application. However, the concentration tended to be lower in rats given OLS than in control rats. With respect to OLS concentration, OLS increased serum dehydroepiandrosterone, secretion concentration-dependently. In addition, as with the recovery study, it tended to inhibit the increase in NEFA. These results indicate that OLS may have an anti-stress activity against acute stress. 相似文献
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T. N. Jacob J. P. Pandey K. Raghuveer G. Sreenivasulu A. D. Gupta M. Yoshikuni A. Jagota B. Senthilkumaran 《Fish physiology and biochemistry》2005,31(2-3):271-274
Effect of experimentally induced thyroxine overdose on the testis and seminal vesicles was studied in the air-breathing catfish,
Clarias gariepinus during the preparatory and the pre-spawning phase. The present study revealed a marked reduction in testosterone level in
serum, testis and seminal vesicles (SV). Histological examination showed a considerable reduction in the number of spermatozoa/spermatids
in the seminiferous tubular lumen as well as depletion of fluid in the loculi of SV. SDS-PAGE analysis of SV fluid proteins
demonstrated a significant decrease in the level of a ~27 kDa protein in thyroxine treated fishes. Evidences are presented
here to indicate that thyroid hormone plays a role in regulating testis and SV function in catfish.
T.N. Jacob and J.P. Pandey contributed equally 相似文献
4.
Based on the strong chelating property of bathophenanthroline disulfonic acid (BPDS) with Fe(Ⅱ), root Fe(Ⅲ) chelate reductase activity is usually measured with a spectrophotometer using MES (2-morpholinoethanesulfonic acid) or HEPES(2-(4-(2-Hydroxyethyl)-l-piperazinyl) ethanesulfonic acid) buffer in the dark because of high autoreduction rate of Fe(Ⅲ)in the presence of light. However, the exclusion of light is inconvenient, especially when analyzing a large number of samples. The objective of this study was to develop a new method for determination of root reductase activity under normal laboratory conditions using a suitable buffer composition and Fe(Ⅲ) concentration to eliminate the autoreduction of Fe(Ⅲ). A modified method using a Tris (2-amino-2-hydroxymethyl-l,3-propanediol) buffer at pH 7.5 instead of MES or HEPES buffer and a decreased FeEDTA (Fe ethylene diamine tetraacetic acid) concentration of 50 μmol L^-1 was developed. The autoreduction of Fe(Ⅲ) using the Tris buffer was undetectable for temperatures at 4 and 28 ℃ and was also much lower than that using the other buffers even with sunlight during measurement of Fe(Ⅲ) reduction.Furthermore, the differences in Fe(Ⅲ) reductase activity among 5 plant species and 14 red clover cultivars (Trifolium pratense L.) could be easily detected with the modified method. The method developed in this study to measure root Fe chelate reductase activity was not only effective and reliable but also easily managed under normal laboratory light conditions. 相似文献
5.
Poly ion complexes of anionic lignosulfonic acid (LSA) with cationic poly(2-vinyl pyridine) or poly(4-vinyl pyridine) were
prepared by mixing the polymer solutions in dimethyl sulfoxide or dilute hydrochloric acid. The complexes were formed as soon
as the polymer solutions were mixed. Fourier transform infrared (FTIR) measurements for the complexes revealed the presence
of strong ionic interactions between sulfonate anions of LSA and pyridinium cations of the vinyl pyridine (VPy) polymers.
The strong interaction was supported by detection of a glass transition temperature (T
g) much higher than pristine T
g values of VPy polymers in differential scanning calorimetry. By using the ionic complexation, a coating of LSA onto VPy polymer
films was undertaken to modify their original surface property. Formation of a thin complex film was confirmed by observation
of absorption bands that are characteristic of the pyridinium cation in reflection FTIR spectra measurements, and by visualization
of the attached LSA component in atomic force microscopy. This treatment resulted in the surface of the VPy polymer films
becoming hydrophobic. An adhesion test was also conducted by pasting two veneered woods with the polymer complex, followed
by shear-tearing them off. The adhesive strength of the complex was estimated to be 1.1 MPa.
Part of this report was presented at the 55th (Kyoto, March 2005) and 56th (Akita, August 2006) Annual Meetings of the Japan
Wood Research Society 相似文献
6.
L.Y. Zhou B. Senthilkumaran D.S. Wang C.C. Sudhakumari T. Kobayashi H. Kajiura-Kobayashi M. Matsuda M. Yoshikuni Y. Nagahama 《Fish physiology and biochemistry》2003,28(1-4):381-382
A 548 bp partial cDNA fragment of 17β-hydroxysteroid dehydrogenase (17β-HSD1) was obtained by RT-PCR from the ovary of Nile Tilapia. The expression of 17β-HSD1 was high from 0 to 11 days after spawning, but there was a sharp decline at the day of spawning (day 14) indicating its involvement in ovarian cycle. 相似文献
7.
D.S. Wang B. Senthilkumaran T. Kobayashi H. Kajiura-Kobayashi M. Matsuda M. Yoshikuni Y. Nagahama 《Fish physiology and biochemistry》2003,28(1-4):225-226
Tilapia riboflavin-binding protein (RfBP) cDNA was isolated by library screening. Even though it shows only 31～35% similarity to the reported RfBPs, tilapia RfBP is conserved in amino acid residues that are known to be essential for its protein function. The exclusive expression of RfBP in the brain-pituitary-gonadal axis, as revealed by both RT-PCR and Northern blot, suggested a possible role for RfBP in fish reproduction. 相似文献
8.
- 1. Ariake Bay, which is located in western Japan, has a large tidal range (>6 m) and a vast tidal flat (200 km2). In the early 1990s, the government‐managed Isahaya Reclamation Project began in the western part of Ariake Bay. A 16‐km2 area of tidal flat in the inner part of Isahaya Bay was destroyed through reclamation and separated from the sea by a dyke, which created land and a freshwater reservoir.
- 2. Since the initiation of the project, fishery yields around Isahaya Bay have dramatically decreased. The objective of this study was to clarify the relationship between the work associated with the Isahaya Reclamation Project and the recent environmental deterioration in Ariake Bay, with references to present sediment thickness and organic matter content, and hypoxic water distributions in Isahaya Bay.
- 3. The organic matter load from the reservoir has increased since the initiation of the reclamation project and has been associated with a thick layer of fine sediment at the bottom of Isahaya Bay. The thickness of fine sediment and the total organic carbon content were higher in Isahaya Bay than in the freshwater reservoir.
- 4. Based on measurements in August 2001, hypoxic water spread widely in and around Isahaya Bay; the lowest dissolved oxygen (DO) concentration (0.53 mg L?1) was observed just outside the dyke. An analysis based on a two‐layered box model using data obtained in August 2001 showed that the DO consumption rate in the bottom layer was high (0.61 mg O2 L?1 day?1), and that 22–41% of the total organic carbon load needed to induce the hypoxic water was derived from the reclamation area.
- 5. Our findings strongly suggest that enclosed seas may suffer from eutrophic and hypoxic conditions because of their low seawater‐exchange rate. A comprehensive conservation programme and environmental assessment including physical and material transport processes in the system is needed to manage the environment of the enclosed sea.
9.
Kaoru YAMAZAKI Takehito SUZUKI Mitsuyuki SHIRAI Tatsuya TAKIZAWA Tadashi SHINODA Toshio MASAOKA Fumiaki AKAHORI Hidetoshi MORITA 《Animal Science Journal》2008,79(3):391-400
The effect of the endocrine-disrupting chemical 3,3',4,4',5-pentachlorobiphyenl (PCB 126) on intestinal microbiota after oral administration, and the improvement of intestinal microbiota and feces quantity by the subsequent administration of Lactobacillus acidophilus or Lactobacillus reuteri was investigated. All the rats were given 100 μg/kg bodyweight of PCB 126. The changes in bacterial counts were confirmed using a culture method. The administration of PCB 126 tended to decrease the bacterial counts of lactobacilli (109.6 −1010.2 to 108.8 −109.2 ) and bifidobacteria (105.3 −106.1 to 103.6 −104.2 ), and to increase those of Enterobacteriaceae (108.2 −109.1 to 109.4 −1010.3 ) and staphylococci (106.6 −107.4 to 107.2 −108.4 ) compared to no PCB 126 administration. After administration of PCB 126, L. acidophilus or L. reuteri orally administered to rats caused Enterobacteriaceae and staphylococci counts to decrease, suggesting that the intestinal microbiota was improved by the lactobacilli. The administration of L. acidophilus and L. reuteri improved the balance of intestinal microbiota, and defecation volume returned to its normal level. L. acidophilus and L. reuteri have a remedial effect on intestinal microbiota affected by PCB 126 and can function to lessen accumulated PCB 126 volume. 相似文献
10.
H.G. Fan D.S. Wang T. Kobayashi B. Senthilkumaran C.C. Sudhakumari M. Yoshikuni Y. Nagahama 《Fish physiology and biochemistry》2003,28(1-4):143-144
Gonadotropin (GTH)α, FSHβ and LHβ cDNAs were cloned from the Nile tilapia. Northern blot analysis detected a single band for each subunit. Preliminary studies indicate that FSHβ is expressed as early as 0 days after hatching (dah) in the fish pituitaries. 相似文献