Evaluation of cross-protection with an attenuated isolate of Bean yellow mosaic virus by differential detection of virus isolates using RT-PCR     

Eiko Nakazono-Nagaoka  Chikako Sato  Yoshitaka Kosaka  Tomohide Natsuaki 《Journal of General Plant Pathology》2004,70(6):359-362

  相似文献   

Comparison of the effects of seed coating with tungsten and molybdenum compounds on seedling establishment rates of rice,wheat, barley,and soybean under flooded conditions     

Yoshitaka Hara 《Plant Production Science》2017,20(4):406-411

Seed coating with molybdenum compounds improves seedling establishment for rice, wheat, barley, and soybean when such seeds were sown under flooded conditions. Tungsten belongs to the same chemical group as molybdenum in the periodic table, and similar to molybdenum, inhibits the generation of sulfide ions. Here, the effects of tungsten and molybdenum containing seed coatings on seedling establishment under flooded conditions were compared using rice, wheat, barley, and soybean. In rice, the effects of tungsten compounds on seedling establishment varied. Tungsten trioxide had little effect but tungstic acid and ammonium phosphotungstate significantly improved seedling establishment when the amounts were at least .1–.2 mol W kg^?1. Although the effect of tungsten coating varied depending on the compound used, ammonium phosphotungstate, along with other tungsten compounds, improved seedling establishment in a manner comparable with that of molybdenum compounds. For wheat and barley, ammonium phosphotungstate treatment resulted in a significant increase in establishment that was only slightly less than the results observed using molybdenum compounds. Tungstic acid and ammonium phosphotungstate treatments improved soybean establishment in a significant manner that was comparable with those of molybdenum compounds. Collectively, these results suggest that tungsten compounds, as well as molybdenum compounds, improve seedling establishment under flooded conditions.  相似文献   

RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus     

Eiko Nakazono-Nagaoka  Masako Suzuki  Yoshitaka Kosaka  Tomohide Natsuaki 《Journal of General Plant Pathology》2005,71(3):243-246

  相似文献   

Proportions of melanocyte stimulating hormone-immunoreactive cells in the adenohypophysis of Silky fowl and hyperpigmentation-free cockerels     

Shotaro NISHIMURA  Hitomi SHIMODA  Ichiro OSHIMA  Yoshitaka ONO  Kaoru OKANO  Akira ISHIBASHI  Shoji TABATA  Hisao IWAMOTO 《Animal Science Journal》2005,76(6):575-579

Alpha‐melanocyte stimulating hormone (α‐MSH) is one of the main regulators for melanocytes, and the adenohypophysis is one of the major tissues for the synthesis of this hormone. The Silky fowl is a characteristic breed of chicken with hyperpigmentation throughout the body. The involvement of the adenohypophysis in the hyperpigmentation of this breed is not known. In the present study, the proportion of melanocyte stimulating hormone‐immunopositive cells (MSH cells) in the adenohypophysis was immunocytochemically compared between Silky, Red Cornish × New Hampshire (RN) crossbred and Japanese bantam cockerels at 15 weeks of age. After the body and gland were weighed, the adenohypophyseal cells were enzymatically dispersed and immunostained for α‐MSH, and the immunopositive MSH cells were counted. The weights of the body and adenohypophysis were heaviest in the RN crossbred, followed by the Silky and lightest in the bantam cockerels. In contrast, the ratio between adenohypophysis and bodyweight was much larger in the Silky and the bantam than in the RN crossbreed (P < 0.05). The population of MSH cells in the adenohypophysis was larger in the Silky (14.3%) than in the RN crossbreed (8.0%) and the bantam (8.1%) cockerels (P < 0.05). From these results, it was concluded that prepubertal Silky cockerel have numerous MSH cells in the adenohypophysis suggesting a relationship to hyperpigmentation.  相似文献   

Does <Emphasis Type="Italic">Puccinia hemerocallidis</Emphasis> regularly host-alternate between <Emphasis Type="Italic">Hemerocallis</Emphasis> and <Emphasis Type="Italic">Patrinia</Emphasis> plants in Japan?     

Yoshitaka?OnoEmail author 《Journal of General Plant Pathology》2003,69(4):240-243

Daylily rust fungus, Puccinia hemerocallidis, was proven to host-alternate between a wild daylily, Hemerocallis fulva var. longituba, and a patrinia, Patrinia villosa. No proof was obtained for the early belief that the fungus is pathogenic to plantainlilies, Hosta species, in addition to daylilies, Hemerocallis species. The fungus seems to alternate regularly between daylilies and patrinias in Japan because most daylily species are deciduous, and a vegetatively reproducing stage of the pathogen does not seem capable of successfully overwintering free of the living host tissue.  相似文献   

Characterization of a lentogenic Newcastle disease virus isolated from broiler chickens in Japan     

Murakawa Y  Takase K  Sakamoto K  Suesoshi M  Nagatomo H 《Avian diseases》2000,44(3):686-690

Newcastle disease virus (NDV), named MET95, was isolated from a non-vaccinated broiler flock in Japan in 1995. The MET95 strain was determined to be a lentogenic NDV. The strain has the properties of eluting rapidly at 4 C and has low thermostability in hemagglutinating activity with chicken erythrocytes. In these studies, no difference could be found between the MET95 strain and the Hitcher B1 vaccine strain. However, the chickens inoculated with the MET95 strain, as well as chickens that they were in contact with, had a much higher hemagglutination-inhibition antibody response than those inoculated with the B1 strain. Accordingly, the MET95 strain is thought to be a promising candidate as a live ND vaccine strain. In Japan, this is the first report on the isolation of lentogenic NDV from chickens since the paper on the Ishii strain isolated in 1966.  相似文献   

Comparison of the nucleotide and amino acid sequences of parental and attenuated isolates of Zucchini yellow mosaic virus     

Wei-Qin Wang  Tomohide Natsuaki  Yoshitaka Kosaka  Seiichi Okuda 《Journal of General Plant Pathology》2006,72(1):52-56

To identify possible sites of viral attenuation, the complete nucleotide sequences of two isolates of Zucchini yellow mosaic virus (ZYMV) were determined; a severe isolate Z5-1 and an attenuated isolate from Z5-1 (designated ZYMV-2002). The viral genome of both isolates consisted of 9593 nucleotides in size and contained an open reading frame encoding a single polyprotein of 3080 amino acids. Comparison of the nucleotide sequences for Z5-1 and ZYMV-2002 revealed 14 nucleotide mutations, resulting in seven amino acid substitutions with four in the HC-Pro region, two in the CI region, and one in the NIb region. These results provide a genetic basis for future manipulation of the ZYMV reverse genetics system. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB188115 and AB188116  相似文献   

Transcript expression profiles of <Emphasis Type="Italic">Takifugu rubripes</Emphasis> spermatozoa and eggs by expressed sequence tag analysis     

Shen XY  Cui JZ  Gong QL  Liu YJ  Nagahama Y 《Fish physiology and biochemistry》2008,34(3):235-243

  相似文献   

Prediction of the buckling stress of intermediate wooden columns using the secant modulus     

Hiroshi Yoshihara  Masamitsu Ohta  Yoshitaka Kubojima 《Journal of Wood Science》1998,44(1):69-72

We made several buckling tests of wooden columns with intermediate slenderness ratios () and examined the empirical formulas. On the basis of the examination, we formulated an equation for predicting the buckling stress () of an intermediate wooden column. Sitka spruce (Picea sitchensis Carr.) and buna (Japanese beech, Fagus crenata BI.) were used for the studies. A compressive load was applied on the specimen supported with pin ends, and the buckling stress was predicted by the tangent modulus theory and two empirical equations: those of Tetmajer and Newlin-Gahagan. The predicted - relations were compared with the test results, and the applicability of these predictions were examined. Based on the comparisons, we formulated an equation that can predict the - relations of materials with various stress-strain characters in the plastic strain range.  相似文献   

Deformation behavior of wood under stress relaxation in a radial direction as a laminated annual-ring structure     

Kosei?AndoEmail author  Mami?Morimoto  Yoshitaka?Kubojima 《Journal of Wood Science》2004,50(4):381-382

  相似文献