耐储藏杂交水稻新品系储藏优1号农艺性状及耐储藏特性研究(英文)   总被引：6，自引：1，他引：5  

张瑛  余行道  朱学贵  宣红  吴跃进  吴敬德 《农业科学与技术》2008,9(4):78-81

[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic characters of the new line and the control II you 838 and Shanyou 63 were comparatively studied,and storage durability of the new line and indica,japonica or hybrid rice were also comparatively analyzed by the accelerated ageing test.[Result] Agronomic characters of the new line were excellent,and germination rate in the artificial accelerated ageing test was significantly higher than those in other materials.[Conclusion] Chucangyou No.1 has the better storage durability,which can be used in large scale of extention.  相似文献   

利用EcoTILLING简化技术进行水稻基因型鉴定及单核苷酸多态性(SNP)检测     

滕斌  李泽福  罗志祥  吴敬德  朱学桂  宣红  张瑛 《农业生物技术学报》2012,20(3):254-260

EcoTILLING(ecotype targeting induced local lesions in genomes)是一种高通量检测和分析自然群体中单核苷酸多态性(single nucleotide polymorphism,SNP)变异的研究方法。由于该技术需要高级、精密的仪器设备和昂贵的荧光标记引物来检测CEL(celery)Ⅰ的酶切产物,极大地限制了该技术的应用。本研究对传统EcoTILLING技术做了适当改进,简化了CELⅠ酶的抽提步骤,采用自然冷却方法制备异源双链产物,普通银染检测CELⅠ酶切产物,酶活检测结果显示CELⅠ粗提液对错配产物切割效果良好。利用EcoTILLING简化技术对18份水稻(Oryza sativa L.)品系Wx基因第1内含子5’端的G/T基因型进行了成功鉴定。此外,对18份水稻材料中一417bp长的Wx基因片段实现了CELⅠ酶切条带分型,经测序验证,酶切带型差异能够准确反映材料间SNP的变异。本研究结果表明,应用EcoTILLING简化技术可有效进行水稻基因型鉴定及SNP检测,是开展水稻功能基因组研究的有力工具。  相似文献   

耐储藏杂交水稻新品系储藏优1号农艺性状及耐储藏特性研究   总被引：1，自引：0，他引：1  

张瑛  余行道  朱学贵  宣红  吴跃进  吴敬德 《安徽农业科学》2008,36(32)

[目的]为探明利用云恢290的耐储藏基因选育的耐储藏杂交水稻新品系储藏优1号的耐储藏特性。[方法]将该品系与对照Ⅱ优838及汕优63的农艺性状进行调查比较,并采用加速老化试验比较其与籼稻、粳稻及杂交水稻材料的耐储藏特性。[结果]该品系农艺性状优异,人工加速老化后发芽率明显高于其他材料。[结论]储藏优1号有较好的耐储藏特性,适于大面积推广。  相似文献